Cargando…

Mitochondrial Population in Mouse Eosinophils: Ultrastructural Dynamics in Cell Differentiation and Inflammatory Diseases

Mitochondria are multifunctional organelles of which ultrastructure is tightly linked to cell physiology. Accumulating evidence shows that mitochondrial remodeling has an impact on immune responses, but our current understanding of the mitochondrial architecture, interactions, and morphological chan...

Descripción completa

Detalles Bibliográficos
Autores principales: Bonjour, Kennedy, Palazzi, Cinthia, Silva, Thiago P., Malta, Kássia K., Neves, Vitor H., Oliveira-Barros, Eliane G., Neves, Igor, Kersten, Victor A., Fortuna, Bruno T., Samarasinghe, Amali E., Weller, Peter F., Bandeira-Melo, Christianne, Melo, Rossana C. N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8979069/
https://www.ncbi.nlm.nih.gov/pubmed/35386204
http://dx.doi.org/10.3389/fcell.2022.836755
_version_ 1784681094576078848
author Bonjour, Kennedy
Palazzi, Cinthia
Silva, Thiago P.
Malta, Kássia K.
Neves, Vitor H.
Oliveira-Barros, Eliane G.
Neves, Igor
Kersten, Victor A.
Fortuna, Bruno T.
Samarasinghe, Amali E.
Weller, Peter F.
Bandeira-Melo, Christianne
Melo, Rossana C. N.
author_facet Bonjour, Kennedy
Palazzi, Cinthia
Silva, Thiago P.
Malta, Kássia K.
Neves, Vitor H.
Oliveira-Barros, Eliane G.
Neves, Igor
Kersten, Victor A.
Fortuna, Bruno T.
Samarasinghe, Amali E.
Weller, Peter F.
Bandeira-Melo, Christianne
Melo, Rossana C. N.
author_sort Bonjour, Kennedy
collection PubMed
description Mitochondria are multifunctional organelles of which ultrastructure is tightly linked to cell physiology. Accumulating evidence shows that mitochondrial remodeling has an impact on immune responses, but our current understanding of the mitochondrial architecture, interactions, and morphological changes in immune cells, mainly in eosinophils, is still poorly known. Here, we applied transmission electron microscopy (TEM), single-cell imaging analysis, and electron tomography, a technique that provides three-dimensional (3D) views at high resolution, to investigate mitochondrial dynamics in mouse eosinophils developing in cultures as well as in the context of inflammatory diseases characterized by recruitment and activation of these cells (mouse models of asthma, H1N1 influenza A virus (IAV) infection, and schistosomiasis mansoni). First, quantitative analyses showed that the mitochondrial area decrease 70% during eosinophil development (from undifferentiated precursor cells to mature eosinophils). Mitophagy, a consistent process revealed by TEM in immature but not in mature eosinophils, is likely operating in mitochondrial clearance during eosinophilopoiesis. Events of mitochondria interaction (inter-organelle membrane contacts) were also detected and quantitated within developing eosinophils and included mitochondria-endoplasmic reticulum, mitochondria-mitochondria, and mitochondria-secretory granules, all of them significantly higher in numbers in immature compared to mature cells. Moreover, single-mitochondrion analyses revealed that as the eosinophil matures, mitochondria cristae significantly increase in number and reshape to lamellar morphology. Eosinophils did not change (asthma) or reduced (IAV and Schistosoma infections) their mitochondrial mass in response to inflammatory diseases. However, asthma and schistosomiasis, but not IAV infection, induced amplification of both cristae numbers and volume in individual mitochondria. Mitochondrial cristae remodeling occurred in all inflammatory conditions with the proportions of mitochondria containing only lamellar or tubular, or mixed cristae (an ultrastructural aspect seen just in tissue eosinophils) depending on the tissue/disease microenvironment. The ability of mitochondria to interact with granules, mainly mobilized ones, was remarkably captured by TEM in eosinophils participating in all inflammatory diseases. Altogether, we demonstrate that the processes of eosinophilopoiesis and inflammation-induced activation interfere with the mitochondrial dynamics within mouse eosinophils leading to cristae remodeling and inter-organelle contacts. The understanding of how mitochondrial dynamics contribute to eosinophil immune functions is an open interesting field to be explored.
format Online
Article
Text
id pubmed-8979069
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-89790692022-04-05 Mitochondrial Population in Mouse Eosinophils: Ultrastructural Dynamics in Cell Differentiation and Inflammatory Diseases Bonjour, Kennedy Palazzi, Cinthia Silva, Thiago P. Malta, Kássia K. Neves, Vitor H. Oliveira-Barros, Eliane G. Neves, Igor Kersten, Victor A. Fortuna, Bruno T. Samarasinghe, Amali E. Weller, Peter F. Bandeira-Melo, Christianne Melo, Rossana C. N. Front Cell Dev Biol Cell and Developmental Biology Mitochondria are multifunctional organelles of which ultrastructure is tightly linked to cell physiology. Accumulating evidence shows that mitochondrial remodeling has an impact on immune responses, but our current understanding of the mitochondrial architecture, interactions, and morphological changes in immune cells, mainly in eosinophils, is still poorly known. Here, we applied transmission electron microscopy (TEM), single-cell imaging analysis, and electron tomography, a technique that provides three-dimensional (3D) views at high resolution, to investigate mitochondrial dynamics in mouse eosinophils developing in cultures as well as in the context of inflammatory diseases characterized by recruitment and activation of these cells (mouse models of asthma, H1N1 influenza A virus (IAV) infection, and schistosomiasis mansoni). First, quantitative analyses showed that the mitochondrial area decrease 70% during eosinophil development (from undifferentiated precursor cells to mature eosinophils). Mitophagy, a consistent process revealed by TEM in immature but not in mature eosinophils, is likely operating in mitochondrial clearance during eosinophilopoiesis. Events of mitochondria interaction (inter-organelle membrane contacts) were also detected and quantitated within developing eosinophils and included mitochondria-endoplasmic reticulum, mitochondria-mitochondria, and mitochondria-secretory granules, all of them significantly higher in numbers in immature compared to mature cells. Moreover, single-mitochondrion analyses revealed that as the eosinophil matures, mitochondria cristae significantly increase in number and reshape to lamellar morphology. Eosinophils did not change (asthma) or reduced (IAV and Schistosoma infections) their mitochondrial mass in response to inflammatory diseases. However, asthma and schistosomiasis, but not IAV infection, induced amplification of both cristae numbers and volume in individual mitochondria. Mitochondrial cristae remodeling occurred in all inflammatory conditions with the proportions of mitochondria containing only lamellar or tubular, or mixed cristae (an ultrastructural aspect seen just in tissue eosinophils) depending on the tissue/disease microenvironment. The ability of mitochondria to interact with granules, mainly mobilized ones, was remarkably captured by TEM in eosinophils participating in all inflammatory diseases. Altogether, we demonstrate that the processes of eosinophilopoiesis and inflammation-induced activation interfere with the mitochondrial dynamics within mouse eosinophils leading to cristae remodeling and inter-organelle contacts. The understanding of how mitochondrial dynamics contribute to eosinophil immune functions is an open interesting field to be explored. Frontiers Media S.A. 2022-03-21 /pmc/articles/PMC8979069/ /pubmed/35386204 http://dx.doi.org/10.3389/fcell.2022.836755 Text en Copyright © 2022 Bonjour, Palazzi, Silva, Malta, Neves, Oliveira-Barros, Neves, Kersten, Fortuna, Samarasinghe, Weller, Bandeira-Melo and Melo. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Bonjour, Kennedy
Palazzi, Cinthia
Silva, Thiago P.
Malta, Kássia K.
Neves, Vitor H.
Oliveira-Barros, Eliane G.
Neves, Igor
Kersten, Victor A.
Fortuna, Bruno T.
Samarasinghe, Amali E.
Weller, Peter F.
Bandeira-Melo, Christianne
Melo, Rossana C. N.
Mitochondrial Population in Mouse Eosinophils: Ultrastructural Dynamics in Cell Differentiation and Inflammatory Diseases
title Mitochondrial Population in Mouse Eosinophils: Ultrastructural Dynamics in Cell Differentiation and Inflammatory Diseases
title_full Mitochondrial Population in Mouse Eosinophils: Ultrastructural Dynamics in Cell Differentiation and Inflammatory Diseases
title_fullStr Mitochondrial Population in Mouse Eosinophils: Ultrastructural Dynamics in Cell Differentiation and Inflammatory Diseases
title_full_unstemmed Mitochondrial Population in Mouse Eosinophils: Ultrastructural Dynamics in Cell Differentiation and Inflammatory Diseases
title_short Mitochondrial Population in Mouse Eosinophils: Ultrastructural Dynamics in Cell Differentiation and Inflammatory Diseases
title_sort mitochondrial population in mouse eosinophils: ultrastructural dynamics in cell differentiation and inflammatory diseases
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8979069/
https://www.ncbi.nlm.nih.gov/pubmed/35386204
http://dx.doi.org/10.3389/fcell.2022.836755
work_keys_str_mv AT bonjourkennedy mitochondrialpopulationinmouseeosinophilsultrastructuraldynamicsincelldifferentiationandinflammatorydiseases
AT palazzicinthia mitochondrialpopulationinmouseeosinophilsultrastructuraldynamicsincelldifferentiationandinflammatorydiseases
AT silvathiagop mitochondrialpopulationinmouseeosinophilsultrastructuraldynamicsincelldifferentiationandinflammatorydiseases
AT maltakassiak mitochondrialpopulationinmouseeosinophilsultrastructuraldynamicsincelldifferentiationandinflammatorydiseases
AT nevesvitorh mitochondrialpopulationinmouseeosinophilsultrastructuraldynamicsincelldifferentiationandinflammatorydiseases
AT oliveirabarroselianeg mitochondrialpopulationinmouseeosinophilsultrastructuraldynamicsincelldifferentiationandinflammatorydiseases
AT nevesigor mitochondrialpopulationinmouseeosinophilsultrastructuraldynamicsincelldifferentiationandinflammatorydiseases
AT kerstenvictora mitochondrialpopulationinmouseeosinophilsultrastructuraldynamicsincelldifferentiationandinflammatorydiseases
AT fortunabrunot mitochondrialpopulationinmouseeosinophilsultrastructuraldynamicsincelldifferentiationandinflammatorydiseases
AT samarasingheamalie mitochondrialpopulationinmouseeosinophilsultrastructuraldynamicsincelldifferentiationandinflammatorydiseases
AT wellerpeterf mitochondrialpopulationinmouseeosinophilsultrastructuraldynamicsincelldifferentiationandinflammatorydiseases
AT bandeiramelochristianne mitochondrialpopulationinmouseeosinophilsultrastructuraldynamicsincelldifferentiationandinflammatorydiseases
AT melorossanacn mitochondrialpopulationinmouseeosinophilsultrastructuraldynamicsincelldifferentiationandinflammatorydiseases