The Effect of Anti-browning Agent Activated Carbon and Polyvinyl Pyrrolidone on the Rooting of Embryo Seedlings of “FengDan” and Its Transcriptome Analysis
Peony is an excellent ornamental, medicinal, and oily plant. Its traditional seed propagation methods have the disadvantages of low propagation coefficient, long seedling cycle, and low seedling emergence rate, which severely restrict the supply of seedlings for the peony industry. Efficient tissue...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8979295/ https://www.ncbi.nlm.nih.gov/pubmed/35386678 http://dx.doi.org/10.3389/fpls.2022.832619 |
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author | Chen, Xia Yu, Chao Nie, Jingtao Yang, Hongmin Ji, Wen Xu, Gangwei Zhu, Huijun Jin, Songheng Zhu, Xiangtao |
author_facet | Chen, Xia Yu, Chao Nie, Jingtao Yang, Hongmin Ji, Wen Xu, Gangwei Zhu, Huijun Jin, Songheng Zhu, Xiangtao |
author_sort | Chen, Xia |
collection | PubMed |
description | Peony is an excellent ornamental, medicinal, and oily plant. Its traditional seed propagation methods have the disadvantages of low propagation coefficient, long seedling cycle, and low seedling emergence rate, which severely restrict the supply of seedlings for the peony industry. Efficient tissue culture technology is an important basis for accelerating its breeding and reproduction, and in vitro seed embryo culturing into seedlings can also effectively avoid the above problems. However, the browning phenomenon caused by man-made damage in the process of seed embryo stripping leads to problems such as low induction rate and difficulty in rooting, and the relationship between anti-browning agents and seed embryo root formation is still unclear. This study intends to improve the induction rate of peony seedlings by using different anti-browning agents and different combinations and to clarify the relationship between anti-browning agents and seedling rooting using transcriptome sequencing methods. The results show that both anti-browning agents, activated carbon (AC) and polyvinyl pyrrolidone (PVP), can increase the germination rate of seed embryos. Testing with 0.9 g/L of AC showed excellent performance of peony rooting rate and seedling growth, but only AC and the combination of AC and PVP can further promote rooting development. Through transcriptome analysis, we found that the AC vs. control check (CK), AC vs. PVP, and PVP vs. AC and PVP groups have significantly more differentially expressed genes than the AC vs. AC and PVP groups. Pathway enrichment analysis shows that “phenylpropanoid biosynthesis”/“cutin, suberin, and wax biosynthesis” is significantly enriched in these groups, while the AC vs. AC and PVP groups are mainly enriched in “cytochrome P450,” indicating that AC may promote the further development of roots into seedlings by stimulating “phenylpropanoid biosynthesis” and biosynthesis of stratum cutin and suberin. This study can lay the foundation for understanding the potential molecular mechanism of the anti-browning agent promoting the rooting of seed embryo seedlings and also provide a theoretical basis for perfecting the construction of the peony tissue culture and rapid propagation system. |
format | Online Article Text |
id | pubmed-8979295 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-89792952022-04-05 The Effect of Anti-browning Agent Activated Carbon and Polyvinyl Pyrrolidone on the Rooting of Embryo Seedlings of “FengDan” and Its Transcriptome Analysis Chen, Xia Yu, Chao Nie, Jingtao Yang, Hongmin Ji, Wen Xu, Gangwei Zhu, Huijun Jin, Songheng Zhu, Xiangtao Front Plant Sci Plant Science Peony is an excellent ornamental, medicinal, and oily plant. Its traditional seed propagation methods have the disadvantages of low propagation coefficient, long seedling cycle, and low seedling emergence rate, which severely restrict the supply of seedlings for the peony industry. Efficient tissue culture technology is an important basis for accelerating its breeding and reproduction, and in vitro seed embryo culturing into seedlings can also effectively avoid the above problems. However, the browning phenomenon caused by man-made damage in the process of seed embryo stripping leads to problems such as low induction rate and difficulty in rooting, and the relationship between anti-browning agents and seed embryo root formation is still unclear. This study intends to improve the induction rate of peony seedlings by using different anti-browning agents and different combinations and to clarify the relationship between anti-browning agents and seedling rooting using transcriptome sequencing methods. The results show that both anti-browning agents, activated carbon (AC) and polyvinyl pyrrolidone (PVP), can increase the germination rate of seed embryos. Testing with 0.9 g/L of AC showed excellent performance of peony rooting rate and seedling growth, but only AC and the combination of AC and PVP can further promote rooting development. Through transcriptome analysis, we found that the AC vs. control check (CK), AC vs. PVP, and PVP vs. AC and PVP groups have significantly more differentially expressed genes than the AC vs. AC and PVP groups. Pathway enrichment analysis shows that “phenylpropanoid biosynthesis”/“cutin, suberin, and wax biosynthesis” is significantly enriched in these groups, while the AC vs. AC and PVP groups are mainly enriched in “cytochrome P450,” indicating that AC may promote the further development of roots into seedlings by stimulating “phenylpropanoid biosynthesis” and biosynthesis of stratum cutin and suberin. This study can lay the foundation for understanding the potential molecular mechanism of the anti-browning agent promoting the rooting of seed embryo seedlings and also provide a theoretical basis for perfecting the construction of the peony tissue culture and rapid propagation system. Frontiers Media S.A. 2022-03-21 /pmc/articles/PMC8979295/ /pubmed/35386678 http://dx.doi.org/10.3389/fpls.2022.832619 Text en Copyright © 2022 Chen, Yu, Nie, Yang, Ji, Xu, Zhu, Jin and Zhu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Chen, Xia Yu, Chao Nie, Jingtao Yang, Hongmin Ji, Wen Xu, Gangwei Zhu, Huijun Jin, Songheng Zhu, Xiangtao The Effect of Anti-browning Agent Activated Carbon and Polyvinyl Pyrrolidone on the Rooting of Embryo Seedlings of “FengDan” and Its Transcriptome Analysis |
title | The Effect of Anti-browning Agent Activated Carbon and Polyvinyl Pyrrolidone on the Rooting of Embryo Seedlings of “FengDan” and Its Transcriptome Analysis |
title_full | The Effect of Anti-browning Agent Activated Carbon and Polyvinyl Pyrrolidone on the Rooting of Embryo Seedlings of “FengDan” and Its Transcriptome Analysis |
title_fullStr | The Effect of Anti-browning Agent Activated Carbon and Polyvinyl Pyrrolidone on the Rooting of Embryo Seedlings of “FengDan” and Its Transcriptome Analysis |
title_full_unstemmed | The Effect of Anti-browning Agent Activated Carbon and Polyvinyl Pyrrolidone on the Rooting of Embryo Seedlings of “FengDan” and Its Transcriptome Analysis |
title_short | The Effect of Anti-browning Agent Activated Carbon and Polyvinyl Pyrrolidone on the Rooting of Embryo Seedlings of “FengDan” and Its Transcriptome Analysis |
title_sort | effect of anti-browning agent activated carbon and polyvinyl pyrrolidone on the rooting of embryo seedlings of “fengdan” and its transcriptome analysis |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8979295/ https://www.ncbi.nlm.nih.gov/pubmed/35386678 http://dx.doi.org/10.3389/fpls.2022.832619 |
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