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Expression and regulation of estrogen receptor 2 and its coregulators in mouse granulosa cells

The cooperative effects of estrogen and oocyte-derived paracrine factors (ODPFs) play critical roles in the normal development of ovarian follicles; however, the mechanism underlying this cooperation has not been well studied. The present study aimed to determine whether ODPFs affect estrogen signal...

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Autores principales: EMORI, Chihiro, KANKE, Takuya, ITO, Haruka, AKIMOTO, Yuki, FUJII, Wataru, NAITO, Kunihiko, SUGIURA, Koji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Society for Reproduction and Development 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8979806/
https://www.ncbi.nlm.nih.gov/pubmed/35046244
http://dx.doi.org/10.1262/jrd.2021-114
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author EMORI, Chihiro
KANKE, Takuya
ITO, Haruka
AKIMOTO, Yuki
FUJII, Wataru
NAITO, Kunihiko
SUGIURA, Koji
author_facet EMORI, Chihiro
KANKE, Takuya
ITO, Haruka
AKIMOTO, Yuki
FUJII, Wataru
NAITO, Kunihiko
SUGIURA, Koji
author_sort EMORI, Chihiro
collection PubMed
description The cooperative effects of estrogen and oocyte-derived paracrine factors (ODPFs) play critical roles in the normal development of ovarian follicles; however, the mechanism underlying this cooperation has not been well studied. The present study aimed to determine whether ODPFs affect estrogen signaling by regulating the expression of estrogen receptor (ESR) and its coregulators in mouse granulosa cells. Some transcripts encoding ESR coregulators were differentially expressed between cumulus and mural granulosa cells (MGCs). The transcript levels of ESR coregulators, including nuclear receptor corepressor 1 and activator 2, in cumulus cells were significantly suppressed by ODPFs; however, they increased when cumulus cell-oocyte complexes were treated with the transforming growth factor beta receptor I inhibitor, SB431542. Moreover, MGCs exhibited significantly higher ESR2 protein and transcript levels than those in cumulus cells. ODPFs promoted Esr2 expression in cumulus cells but had no effect on that in MGCs. Overall, regulation of the expression of ESR2 and its coregulators in cumulus cells by oocytes seems to be one of the mechanisms underlying estrogen-oocyte cooperation in well-developed antral follicles in mice.
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spelling pubmed-89798062022-04-14 Expression and regulation of estrogen receptor 2 and its coregulators in mouse granulosa cells EMORI, Chihiro KANKE, Takuya ITO, Haruka AKIMOTO, Yuki FUJII, Wataru NAITO, Kunihiko SUGIURA, Koji J Reprod Dev Original Article The cooperative effects of estrogen and oocyte-derived paracrine factors (ODPFs) play critical roles in the normal development of ovarian follicles; however, the mechanism underlying this cooperation has not been well studied. The present study aimed to determine whether ODPFs affect estrogen signaling by regulating the expression of estrogen receptor (ESR) and its coregulators in mouse granulosa cells. Some transcripts encoding ESR coregulators were differentially expressed between cumulus and mural granulosa cells (MGCs). The transcript levels of ESR coregulators, including nuclear receptor corepressor 1 and activator 2, in cumulus cells were significantly suppressed by ODPFs; however, they increased when cumulus cell-oocyte complexes were treated with the transforming growth factor beta receptor I inhibitor, SB431542. Moreover, MGCs exhibited significantly higher ESR2 protein and transcript levels than those in cumulus cells. ODPFs promoted Esr2 expression in cumulus cells but had no effect on that in MGCs. Overall, regulation of the expression of ESR2 and its coregulators in cumulus cells by oocytes seems to be one of the mechanisms underlying estrogen-oocyte cooperation in well-developed antral follicles in mice. The Society for Reproduction and Development 2022-01-18 2022-04 /pmc/articles/PMC8979806/ /pubmed/35046244 http://dx.doi.org/10.1262/jrd.2021-114 Text en ©2022 Society for Reproduction and Development https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Original Article
EMORI, Chihiro
KANKE, Takuya
ITO, Haruka
AKIMOTO, Yuki
FUJII, Wataru
NAITO, Kunihiko
SUGIURA, Koji
Expression and regulation of estrogen receptor 2 and its coregulators in mouse granulosa cells
title Expression and regulation of estrogen receptor 2 and its coregulators in mouse granulosa cells
title_full Expression and regulation of estrogen receptor 2 and its coregulators in mouse granulosa cells
title_fullStr Expression and regulation of estrogen receptor 2 and its coregulators in mouse granulosa cells
title_full_unstemmed Expression and regulation of estrogen receptor 2 and its coregulators in mouse granulosa cells
title_short Expression and regulation of estrogen receptor 2 and its coregulators in mouse granulosa cells
title_sort expression and regulation of estrogen receptor 2 and its coregulators in mouse granulosa cells
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8979806/
https://www.ncbi.nlm.nih.gov/pubmed/35046244
http://dx.doi.org/10.1262/jrd.2021-114
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