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A multipathogen DNA vaccine elicits protective immune responses against two class A bioterrorism agents, anthrax and botulism

ABSTRACT: The potential use of biological agents has become a major public health concern worldwide. According to the CDC classification, Bacillus anthracis and Clostridium botulinum, the bacterial pathogens that cause anthrax and botulism, respectively, are considered to be the most dangerous poten...

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Autores principales: Kim, Na Young, Son, Won Rak, Lee, Min Hoon, Choi, Hong Seok, Choi, Jun Young, Song, Young Jo, Yu, Chi Ho, Song, Dong Hyun, Hur, Gyeung Haeng, Jeong, Seong Tae, Hong, Sung Youl, Shin, Young Kee, Shin, Sungho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8979915/
https://www.ncbi.nlm.nih.gov/pubmed/35141866
http://dx.doi.org/10.1007/s00253-022-11812-6
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author Kim, Na Young
Son, Won Rak
Lee, Min Hoon
Choi, Hong Seok
Choi, Jun Young
Song, Young Jo
Yu, Chi Ho
Song, Dong Hyun
Hur, Gyeung Haeng
Jeong, Seong Tae
Hong, Sung Youl
Shin, Young Kee
Shin, Sungho
author_facet Kim, Na Young
Son, Won Rak
Lee, Min Hoon
Choi, Hong Seok
Choi, Jun Young
Song, Young Jo
Yu, Chi Ho
Song, Dong Hyun
Hur, Gyeung Haeng
Jeong, Seong Tae
Hong, Sung Youl
Shin, Young Kee
Shin, Sungho
author_sort Kim, Na Young
collection PubMed
description ABSTRACT: The potential use of biological agents has become a major public health concern worldwide. According to the CDC classification, Bacillus anthracis and Clostridium botulinum, the bacterial pathogens that cause anthrax and botulism, respectively, are considered to be the most dangerous potential biological agents. Currently, there is no licensed vaccine that is well suited for mass immunization in the event of an anthrax or botulism epidemic. In the present study, we developed a dual-expression system-based multipathogen DNA vaccine that encodes the PA-D4 gene of B. anthracis and the HCt gene of C. botulinum. When the multipathogen DNA vaccine was administered to mice and guinea pigs, high level antibody responses were elicited against both PA-D4 and HCt. Analysis of the serum IgG subtype implied a combined Th1/Th2 response to both antigens, but one that was Th2 skewed. In addition, immunization with the multipathogen DNA vaccine induced effective neutralizing antibody activity against both PA-D4 and HCt. Finally, the protection efficiency of the multipathogen DNA vaccine was determined by sequential challenge with 10 LD(50) of B. anthracis spores and 10 LD(50) of botulinum toxin, or vice versa, and the multipathogen DNA vaccine provided higher than 50% protection against lethal challenge with both high-risk biothreat agents. Our studies suggest the strategy used for this anthrax-botulinum multipathogen DNA vaccine as a prospective approach for developing emergency vaccines that can be immediately distributed on a massive scale in response to a biothreat emergency or infectious disease outbreak. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00253-022-11812-6.
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spelling pubmed-89799152022-04-05 A multipathogen DNA vaccine elicits protective immune responses against two class A bioterrorism agents, anthrax and botulism Kim, Na Young Son, Won Rak Lee, Min Hoon Choi, Hong Seok Choi, Jun Young Song, Young Jo Yu, Chi Ho Song, Dong Hyun Hur, Gyeung Haeng Jeong, Seong Tae Hong, Sung Youl Shin, Young Kee Shin, Sungho Appl Microbiol Biotechnol Applied Genetics and Molecular Biotechnology ABSTRACT: The potential use of biological agents has become a major public health concern worldwide. According to the CDC classification, Bacillus anthracis and Clostridium botulinum, the bacterial pathogens that cause anthrax and botulism, respectively, are considered to be the most dangerous potential biological agents. Currently, there is no licensed vaccine that is well suited for mass immunization in the event of an anthrax or botulism epidemic. In the present study, we developed a dual-expression system-based multipathogen DNA vaccine that encodes the PA-D4 gene of B. anthracis and the HCt gene of C. botulinum. When the multipathogen DNA vaccine was administered to mice and guinea pigs, high level antibody responses were elicited against both PA-D4 and HCt. Analysis of the serum IgG subtype implied a combined Th1/Th2 response to both antigens, but one that was Th2 skewed. In addition, immunization with the multipathogen DNA vaccine induced effective neutralizing antibody activity against both PA-D4 and HCt. Finally, the protection efficiency of the multipathogen DNA vaccine was determined by sequential challenge with 10 LD(50) of B. anthracis spores and 10 LD(50) of botulinum toxin, or vice versa, and the multipathogen DNA vaccine provided higher than 50% protection against lethal challenge with both high-risk biothreat agents. Our studies suggest the strategy used for this anthrax-botulinum multipathogen DNA vaccine as a prospective approach for developing emergency vaccines that can be immediately distributed on a massive scale in response to a biothreat emergency or infectious disease outbreak. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00253-022-11812-6. Springer Berlin Heidelberg 2022-02-10 2022 /pmc/articles/PMC8979915/ /pubmed/35141866 http://dx.doi.org/10.1007/s00253-022-11812-6 Text en © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2022 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Applied Genetics and Molecular Biotechnology
Kim, Na Young
Son, Won Rak
Lee, Min Hoon
Choi, Hong Seok
Choi, Jun Young
Song, Young Jo
Yu, Chi Ho
Song, Dong Hyun
Hur, Gyeung Haeng
Jeong, Seong Tae
Hong, Sung Youl
Shin, Young Kee
Shin, Sungho
A multipathogen DNA vaccine elicits protective immune responses against two class A bioterrorism agents, anthrax and botulism
title A multipathogen DNA vaccine elicits protective immune responses against two class A bioterrorism agents, anthrax and botulism
title_full A multipathogen DNA vaccine elicits protective immune responses against two class A bioterrorism agents, anthrax and botulism
title_fullStr A multipathogen DNA vaccine elicits protective immune responses against two class A bioterrorism agents, anthrax and botulism
title_full_unstemmed A multipathogen DNA vaccine elicits protective immune responses against two class A bioterrorism agents, anthrax and botulism
title_short A multipathogen DNA vaccine elicits protective immune responses against two class A bioterrorism agents, anthrax and botulism
title_sort multipathogen dna vaccine elicits protective immune responses against two class a bioterrorism agents, anthrax and botulism
topic Applied Genetics and Molecular Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8979915/
https://www.ncbi.nlm.nih.gov/pubmed/35141866
http://dx.doi.org/10.1007/s00253-022-11812-6
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