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Chemiluminescent screening of specific hybridoma cells via a proximity-rolling circle activated enzymatic switch
The mass-production capability of hybridoma technology is bottlenecked by the routine screening procedure which is time-consuming and laborious as the requirement of clonal expansion. Here, we describe a 1-day chemiluminescent screening protocol for specific hybridoma cells on conventional 96-well p...
Autores principales: | Ao, Hang, Chen, Weiwei, Wu, Jie, Xiao, Wencheng, Ju, Huangxian |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8979942/ https://www.ncbi.nlm.nih.gov/pubmed/35379898 http://dx.doi.org/10.1038/s42003-022-03283-2 |
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