Triple gene editing in porcine embryos using electroporation alone or in combination with microinjection

BACKGROUND AND AIM: We previously developed the gene-editing by electroporation (EP) of Cas9 protein method, in which the CRISPR/Cas9 system was introduced into porcine in vitro fertilized (IVF) zygotes through EP to disrupt a target gene. This method should be further developed, and a combination o...

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Detalles Bibliográficos
Autores principales: Namula, Zhao, Le, Quynh Anh, Wittayarat, Manita, Lin, Qingyi, Takebayashi, Koki, Hirata, Maki, Do, Lanh Thi Kim, Tanihara, Fuminori, Otoi, Takeshige
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Veterinary World 2022
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8980404/
https://www.ncbi.nlm.nih.gov/pubmed/35400948
http://dx.doi.org/10.14202/vetworld.2022.496-501

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8980404/
https://www.ncbi.nlm.nih.gov/pubmed/35400948
http://dx.doi.org/10.14202/vetworld.2022.496-501

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