Cargando…

Triple gene editing in porcine embryos using electroporation alone or in combination with microinjection

BACKGROUND AND AIM: We previously developed the gene-editing by electroporation (EP) of Cas9 protein method, in which the CRISPR/Cas9 system was introduced into porcine in vitro fertilized (IVF) zygotes through EP to disrupt a target gene. This method should be further developed, and a combination o...

Descripción completa

Detalles Bibliográficos
Autores principales:	Namula, Zhao, Le, Quynh Anh, Wittayarat, Manita, Lin, Qingyi, Takebayashi, Koki, Hirata, Maki, Do, Lanh Thi Kim, Tanihara, Fuminori, Otoi, Takeshige
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Veterinary World 2022
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8980404/ https://www.ncbi.nlm.nih.gov/pubmed/35400948 http://dx.doi.org/10.14202/vetworld.2022.496-501

Ejemplares similares

Multiple gene editing in porcine embryos using a combination of microinjection, electroporation, and transfection methods
por: Le, Quynh Anh, et al.
Publicado: (2022)

Comparison of the effects of introducing the CRISPR/Cas9 system by microinjection and electroporation into porcine embryos at different stages
por: Le, Quynh Anh, et al.
Publicado: (2021)

Effects of the timing of electroporation during in vitro maturation on triple gene editing in porcine embryos using CRISPR/Cas9 system
por: Namula, Zhao, et al.
Publicado: (2022)

Generation of mutant pigs by lipofection-mediated genome editing in embryos
por: Hirata, Maki, et al.
Publicado: (2021)

Lipofection-Mediated Introduction of CRISPR/Cas9 System into Porcine Oocytes and Embryos
por: Hirata, Maki, et al.
Publicado: (2021)

Timing and duration of lipofection-mediated CRISPR/Cas9 delivery into porcine zygotes affect gene-editing events
por: Lin, Qingyi, et al.
Publicado: (2021)

Pigs with an INS point mutation derived from zygotes electroporated with CRISPR/Cas9 and ssODN
por: Tanihara, Fuminori, et al.
Publicado: (2023)

The Relationship between Embryonic Development and the Efficiency of Target Mutations in Porcine Endogenous Retroviruses (PERVs) Pol Genes in Porcine Embryos
por: Hirata, Maki, et al.
Publicado: (2019)

Zona pellucida treatment before CRISPR/Cas9‐mediated genome editing of porcine zygotes
por: Namula, Zhao, et al.
Publicado: (2021)

Improvement of the in vitro fertilization and embryo development using frozen–thawed spermatozoa of microminipigs
por: Namula, Zhao, et al.
Publicado: (2021)

Effects of concentration of CRISPR/Cas9 components on genetic mosaicism in cytoplasmic microinjected porcine embryos
por: TANIHARA, Fuminori, et al.
Publicado: (2019)

Current status of the application of gene editing in pigs
por: TANIHARA, Fuminori, et al.
Publicado: (2021)

One-Step Generation of Multiple Gene-Edited Pigs by Electroporation of the CRISPR/Cas9 System into Zygotes to Reduce Xenoantigen Biosynthesis
por: Tanihara, Fuminori, et al.
Publicado: (2021)

The effects of electroporation on viability and quality of in vivo-derived bovine blastocysts
por: TANIHARA, Fuminori, et al.
Publicado: (2019)

Generation of a TP53-modified porcine cancer model by CRISPR/Cas9-mediated gene modification in porcine zygotes via electroporation
por: Tanihara, Fuminori, et al.
Publicado: (2018)

Efficient generation of GGTA1-deficient pigs by electroporation of the CRISPR/Cas9 system into in vitro-fertilized zygotes
por: Tanihara, Fuminori, et al.
Publicado: (2020)

Effect of ferulic acid supplementation on the developmental competence of porcine embryos during in vitro maturation
por: TANIHARA, Fuminori, et al.
Publicado: (2018)

The optimal period of Ca-EDTA treatment for parthenogenetic activation of porcine oocytes during maturation culture
por: MORITA, Yasuhiro, et al.
Publicado: (2016)

Embryo Microinjection and Electroporation in the Chordate Ciona intestinalis
por: Kari, Willi, et al.
Publicado: (2016)

Follicular development of canine ovaries stimulated by a combination treatment of eCG and hCG
por: Hirata, Maki, et al.
Publicado: (2018)

Mosaic TP53 Mutation on Tumour Development in Pigs: A Case Study
por: Thongkittidilok, Chommanart, et al.
Publicado: (2023)

Transgenic expression in zebrafish embryos with an intact chorion by electroporation and microinjection
por: Tazin, Nusrat, et al.
Publicado: (2023)

The Effects of an In Vitro Oocyte Maturation System and Chlorogenic Acid Supplementation during Embryo Culture on the Development of Porcine Cloned Embryos Derived from Native Vietnamese Ban Pigs
por: Nguyen, Thanh Van, et al.
Publicado: (2023)

Vaginal stimulation enhances ovulation of queen ovaries treated using a combination of eCG and hCG
por: Yoshimura, Naoaki, et al.
Publicado: (2021)

Evaluation of Zona Pellucida Function for Sperm Penetration During In Vitro Fertilization in Pigs
por: TANIHARA, Fuminori, et al.
Publicado: (2013)

Optimization of sand fly embryo microinjection for gene editing by CRISPR/Cas9
por: Martin-Martin, Ines, et al.
Publicado: (2018)

Simple Genome Editing of Rodent Intact Embryos by Electroporation
por: Kaneko, Takehito, et al.
Publicado: (2015)

Single-Step Genome Editing of Small Ruminant Embryos by Electroporation
por: Mahdi, Ahmed K., et al.
Publicado: (2022)

Microinjection for precision genome editing in Caenorhabditis elegans
por: Ghanta, Krishna S., et al.
Publicado: (2021)

A Review of Automated Microinjection of Zebrafish Embryos
por: Zhao, Yuliang, et al.
Publicado: (2018)

Production of genome-edited mice by visualization of nucleases introduced into the embryos using electroporation
por: WAKE, Yui, et al.
Publicado: (2020)

Comparative Effects of Different Dosages of hCG on Follicular Development in Postpartum Dairy Cows With Cystic Ovarian Follicles
por: Ono, Tetsushi, et al.
Publicado: (2018)

Embryo microinjection of the lecithotrophic sea urchin Heliocidaris erythrogramma
por: Edgar, Allison, et al.
Publicado: (2019)

Electroporation and Microinjection Successfully Deliver Single-Stranded and Duplex DNA into Live Cells as Detected by FRET Measurements
por: Bamford, Rosemary A., et al.
Publicado: (2014)

A Fully Automated Robotic System for Microinjection of Zebrafish Embryos
por: Wang, Wenhui, et al.
Publicado: (2007)

Embryo Microinjection and Transplantation Technique for Nasonia vitripennis Genome Manipulation
por: Li, Ming, et al.
Publicado: (2017)

Somatic cell reprogramming-free generation of genetically modified pigs
por: Tanihara, Fuminori, et al.
Publicado: (2016)

Amount of Cas9 protein introduced into mouse embryos via electroporation affects the genome-editing rate
por: WAKE, Yui, et al.
Publicado: (2022)

Microinjection-based System for In Vivo Implantation of Embryonic Cardiomyocytes in the Avian Embryo
por: Henley, Trevor, et al.
Publicado: (2019)

The Exact Timing of Microinjection of Parthenogenetic Silkworm Embryos Is Crucial for Their Successful Transgenesis
por: Zabelina, Valeriya, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_8tbdqfakfhdh22ggmkkuiildtq