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Programmed Topographic Substrates for Studying Roughness Gradient-Dependent Cell Migration Using Two-Photon Polymerization

The mediation of the extracellular matrix is one of the major environmental cues to direct cell migration, such as stiffness-dependent durotaxis and adhesiveness-dependent haptotaxis. In this study, we explore another possible contact guidance: roughness dependent topotaxis. Different from previousl...

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Autores principales: Shivani, Subhashree, Hsu, Yu-Hsiang, Lee, Cheng-Je, Cheong, Chi-Sheng, Chung, Tien-Tung, Wang, An-Bang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8980465/
https://www.ncbi.nlm.nih.gov/pubmed/35392174
http://dx.doi.org/10.3389/fcell.2022.825791
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author Shivani, Subhashree
Hsu, Yu-Hsiang
Lee, Cheng-Je
Cheong, Chi-Sheng
Chung, Tien-Tung
Wang, An-Bang
author_facet Shivani, Subhashree
Hsu, Yu-Hsiang
Lee, Cheng-Je
Cheong, Chi-Sheng
Chung, Tien-Tung
Wang, An-Bang
author_sort Shivani, Subhashree
collection PubMed
description The mediation of the extracellular matrix is one of the major environmental cues to direct cell migration, such as stiffness-dependent durotaxis and adhesiveness-dependent haptotaxis. In this study, we explore another possible contact guidance: roughness dependent topotaxis. Different from previously reported studies on topotaxis that use standard photolithography to create micron or submicron structures that have identical height and different spatial densities, we develop a new method to programmatically fabricate substrates with different patterns of surface roughness using two-photon polymerization. Surface roughness ranging from 0.29 to 1.11 μm can be created by controlling the voxel distance between adjacently cured ellipsoid voxels. Patterned Ormocomp(®) masters are transferred to polypropylene films using the nanoimprinting method for cell migration study. Our experimental results suggest that MG63 cells can sense the spatial distribution of their underlying extracellar roughness and modulate their migration velocity and direction. Three characteristic behaviors were identified. First, cells have a higher migration velocity on substrates with higher roughness. Second, cells preferred to migrate from regions of higher roughness to lower roughness, and their migration velocity also decreased with descending roughness. Third, the migration velocity remained unchanged on the lower roughness range on a graded substrate with a steeper roughness. The last cell migration characteristic suggests the steepness of the roughness gradient can be another environmental cue in addition to surface roughness. Finally, the combination of two-photon polymerization and nanoimprint methods could become a new fabrication methodology to create better 3D intricate structures for exploring topotactic cell migrations.
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spelling pubmed-89804652022-04-06 Programmed Topographic Substrates for Studying Roughness Gradient-Dependent Cell Migration Using Two-Photon Polymerization Shivani, Subhashree Hsu, Yu-Hsiang Lee, Cheng-Je Cheong, Chi-Sheng Chung, Tien-Tung Wang, An-Bang Front Cell Dev Biol Cell and Developmental Biology The mediation of the extracellular matrix is one of the major environmental cues to direct cell migration, such as stiffness-dependent durotaxis and adhesiveness-dependent haptotaxis. In this study, we explore another possible contact guidance: roughness dependent topotaxis. Different from previously reported studies on topotaxis that use standard photolithography to create micron or submicron structures that have identical height and different spatial densities, we develop a new method to programmatically fabricate substrates with different patterns of surface roughness using two-photon polymerization. Surface roughness ranging from 0.29 to 1.11 μm can be created by controlling the voxel distance between adjacently cured ellipsoid voxels. Patterned Ormocomp(®) masters are transferred to polypropylene films using the nanoimprinting method for cell migration study. Our experimental results suggest that MG63 cells can sense the spatial distribution of their underlying extracellar roughness and modulate their migration velocity and direction. Three characteristic behaviors were identified. First, cells have a higher migration velocity on substrates with higher roughness. Second, cells preferred to migrate from regions of higher roughness to lower roughness, and their migration velocity also decreased with descending roughness. Third, the migration velocity remained unchanged on the lower roughness range on a graded substrate with a steeper roughness. The last cell migration characteristic suggests the steepness of the roughness gradient can be another environmental cue in addition to surface roughness. Finally, the combination of two-photon polymerization and nanoimprint methods could become a new fabrication methodology to create better 3D intricate structures for exploring topotactic cell migrations. Frontiers Media S.A. 2022-03-22 /pmc/articles/PMC8980465/ /pubmed/35392174 http://dx.doi.org/10.3389/fcell.2022.825791 Text en Copyright © 2022 Shivani, Hsu, Lee, Cheong, Chung and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Shivani, Subhashree
Hsu, Yu-Hsiang
Lee, Cheng-Je
Cheong, Chi-Sheng
Chung, Tien-Tung
Wang, An-Bang
Programmed Topographic Substrates for Studying Roughness Gradient-Dependent Cell Migration Using Two-Photon Polymerization
title Programmed Topographic Substrates for Studying Roughness Gradient-Dependent Cell Migration Using Two-Photon Polymerization
title_full Programmed Topographic Substrates for Studying Roughness Gradient-Dependent Cell Migration Using Two-Photon Polymerization
title_fullStr Programmed Topographic Substrates for Studying Roughness Gradient-Dependent Cell Migration Using Two-Photon Polymerization
title_full_unstemmed Programmed Topographic Substrates for Studying Roughness Gradient-Dependent Cell Migration Using Two-Photon Polymerization
title_short Programmed Topographic Substrates for Studying Roughness Gradient-Dependent Cell Migration Using Two-Photon Polymerization
title_sort programmed topographic substrates for studying roughness gradient-dependent cell migration using two-photon polymerization
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8980465/
https://www.ncbi.nlm.nih.gov/pubmed/35392174
http://dx.doi.org/10.3389/fcell.2022.825791
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