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Generation of a CHIP isogenic human iPSC-derived cortical neuron model for functional proteomics

The neuroprotective E3-ubiquitin ligase CHIP is linked to healthy aging. Here, we present a protocol using a patient-derived iPSC line with a triplication of the α-synuclein gene to produce gene-edited cells isogenic for CHIP. We describe iPSC differentiation into cortical neurons and their identity...

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Detalles Bibliográficos
Autores principales:	Dias, Catarina, Nita, Erisa, Faktor, Jakub, Hernychova, Lenka, Kunath, Tilo, Ball, Kathryn L.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8980993/ https://www.ncbi.nlm.nih.gov/pubmed/35391935 http://dx.doi.org/10.1016/j.xpro.2022.101247

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