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Novel urea derivative-loaded PLGA nanoparticles to inhibit caries-associated Streptococcus mutans

Caries is the most common chronic infectious disease in the human oral cavity and the existing anti-caries agents may lead to drug resistance and microecological imbalance. A novel urea derivative, 1,3-bis[3,5-bis(trifluoromethyl)phenyl]urea, has a potentially prominent antibacterial effect on cario...

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Detalles Bibliográficos
Autores principales: Zhang, Mengyun, Liao, Ying, Tong, Xin, Yan, Fuhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8981093/
https://www.ncbi.nlm.nih.gov/pubmed/35425421
http://dx.doi.org/10.1039/d1ra09314b
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author Zhang, Mengyun
Liao, Ying
Tong, Xin
Yan, Fuhua
author_facet Zhang, Mengyun
Liao, Ying
Tong, Xin
Yan, Fuhua
author_sort Zhang, Mengyun
collection PubMed
description Caries is the most common chronic infectious disease in the human oral cavity and the existing anti-caries agents may lead to drug resistance and microecological imbalance. A novel urea derivative, 1,3-bis[3,5-bis(trifluoromethyl)phenyl]urea, has a potentially prominent antibacterial effect on cariogenic bacterial strain Streptococcus mutans UA159. In this study, we encapsulated the water-insoluble urea derivative in poly(lactic-co-glycolic acid) (PLGA) nanoparticles, performed physicochemical characterizations and explored its potential as a caries-preventive agent. The results showed that the drug-loaded PLGA nanoparticles exhibited satisfying surface morphology, particle size, size distribution and stability. With an optimized theoretical drug loading (10%), the drug-loaded PLGA nanoparticles exhibited negligible cytotoxicity against human oral squamous cell carcinoma cells. We noticed a biphasic drug release in vitro and the rate and cumulative release was higher in an acidic environment (pH 4.5) compared to a neutral environment (pH 7.4). The drug-loaded PLGA nanoparticles significantly inhibited the growth and lactic acid production of planktonic S. mutans as well as S. mutans biofilms. Our results indicate that the novel urea derivative-loaded PLGA nanoparticles serve as a promising anti-caries agent with remarkable pharmaceutical characteristics, low cytotoxicity, and satisfying antimicrobial effect.
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spelling pubmed-89810932022-04-13 Novel urea derivative-loaded PLGA nanoparticles to inhibit caries-associated Streptococcus mutans Zhang, Mengyun Liao, Ying Tong, Xin Yan, Fuhua RSC Adv Chemistry Caries is the most common chronic infectious disease in the human oral cavity and the existing anti-caries agents may lead to drug resistance and microecological imbalance. A novel urea derivative, 1,3-bis[3,5-bis(trifluoromethyl)phenyl]urea, has a potentially prominent antibacterial effect on cariogenic bacterial strain Streptococcus mutans UA159. In this study, we encapsulated the water-insoluble urea derivative in poly(lactic-co-glycolic acid) (PLGA) nanoparticles, performed physicochemical characterizations and explored its potential as a caries-preventive agent. The results showed that the drug-loaded PLGA nanoparticles exhibited satisfying surface morphology, particle size, size distribution and stability. With an optimized theoretical drug loading (10%), the drug-loaded PLGA nanoparticles exhibited negligible cytotoxicity against human oral squamous cell carcinoma cells. We noticed a biphasic drug release in vitro and the rate and cumulative release was higher in an acidic environment (pH 4.5) compared to a neutral environment (pH 7.4). The drug-loaded PLGA nanoparticles significantly inhibited the growth and lactic acid production of planktonic S. mutans as well as S. mutans biofilms. Our results indicate that the novel urea derivative-loaded PLGA nanoparticles serve as a promising anti-caries agent with remarkable pharmaceutical characteristics, low cytotoxicity, and satisfying antimicrobial effect. The Royal Society of Chemistry 2022-02-02 /pmc/articles/PMC8981093/ /pubmed/35425421 http://dx.doi.org/10.1039/d1ra09314b Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Zhang, Mengyun
Liao, Ying
Tong, Xin
Yan, Fuhua
Novel urea derivative-loaded PLGA nanoparticles to inhibit caries-associated Streptococcus mutans
title Novel urea derivative-loaded PLGA nanoparticles to inhibit caries-associated Streptococcus mutans
title_full Novel urea derivative-loaded PLGA nanoparticles to inhibit caries-associated Streptococcus mutans
title_fullStr Novel urea derivative-loaded PLGA nanoparticles to inhibit caries-associated Streptococcus mutans
title_full_unstemmed Novel urea derivative-loaded PLGA nanoparticles to inhibit caries-associated Streptococcus mutans
title_short Novel urea derivative-loaded PLGA nanoparticles to inhibit caries-associated Streptococcus mutans
title_sort novel urea derivative-loaded plga nanoparticles to inhibit caries-associated streptococcus mutans
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8981093/
https://www.ncbi.nlm.nih.gov/pubmed/35425421
http://dx.doi.org/10.1039/d1ra09314b
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