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Well-Plate μFASP for Proteomic Analysis of Single Pancreatic Islets

[Image: see text] Filter-aided sample preparation (FASP) is widely used in bottom-up proteomics for tryptic digestion. However, the sample recovery yield of this method is limited by the amount of the starting material. While ∼100 ng of digested protein is sufficient for thorough protein identificat...

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Detalles Bibliográficos
Autores principales: Sandbaumhüter, Friederike A., Nezhyva, Mariya, Eriksson, Olle, Engberg, Adam, Kreuger, Johan, Andrén, Per E., Jansson, Erik T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8981318/
https://www.ncbi.nlm.nih.gov/pubmed/35293755
http://dx.doi.org/10.1021/acs.jproteome.2c00047