Cargando…

Under‐Oil Autonomously Regulated Oxygen Microenvironments: A Goldilocks Principle‐Based Approach for Microscale Cell Culture

Oxygen levels in vivo are autonomously regulated by a supply–demand balance, which can be altered in disease states. However, the oxygen levels of in vitro cell culture systems, particularly microscale cell culture, are typically dominated by either supply or demand. Further, the oxygen microenviron...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Chao, Humayun, Mouhita, Walker, Glenn M., Park, Keon Young, Connors, Bryce, Feng, Jun, Pellitteri Hahn, Molly C., Scarlett, Cameron O., Li, Jiayi, Feng, Yanbo, Clark, Ryan L., Hefti, Hunter, Schrope, Jonathan, Venturelli, Ophelia S., Beebe, David J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8981459/
https://www.ncbi.nlm.nih.gov/pubmed/35118834
http://dx.doi.org/10.1002/advs.202104510
_version_ 1784681609359785984
author Li, Chao
Humayun, Mouhita
Walker, Glenn M.
Park, Keon Young
Connors, Bryce
Feng, Jun
Pellitteri Hahn, Molly C.
Scarlett, Cameron O.
Li, Jiayi
Feng, Yanbo
Clark, Ryan L.
Hefti, Hunter
Schrope, Jonathan
Venturelli, Ophelia S.
Beebe, David J.
author_facet Li, Chao
Humayun, Mouhita
Walker, Glenn M.
Park, Keon Young
Connors, Bryce
Feng, Jun
Pellitteri Hahn, Molly C.
Scarlett, Cameron O.
Li, Jiayi
Feng, Yanbo
Clark, Ryan L.
Hefti, Hunter
Schrope, Jonathan
Venturelli, Ophelia S.
Beebe, David J.
author_sort Li, Chao
collection PubMed
description Oxygen levels in vivo are autonomously regulated by a supply–demand balance, which can be altered in disease states. However, the oxygen levels of in vitro cell culture systems, particularly microscale cell culture, are typically dominated by either supply or demand. Further, the oxygen microenvironment in these systems is rarely monitored or reported. Here, a method to establish and dynamically monitor autonomously regulated oxygen microenvironments (AROM) using an oil overlay in an open microscale cell culture system is presented. Using this method, the oxygen microenvironment is dynamically regulated via the supply–demand balance of the system. Numerical simulation and experimental validation of oxygen transport within multi‐liquid‐phase, microscale culture systems involving a variety of cell types, including mammalian, fungal, and bacterial cells are presented. Finally, AROM is applied to establish a coculture between cells with disparate oxygen demands—primary intestinal epithelial cells (oxygen consuming) and Bacteroides uniformis (an anaerobic species prevalent in the human gut).
format Online
Article
Text
id pubmed-8981459
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-89814592022-04-11 Under‐Oil Autonomously Regulated Oxygen Microenvironments: A Goldilocks Principle‐Based Approach for Microscale Cell Culture Li, Chao Humayun, Mouhita Walker, Glenn M. Park, Keon Young Connors, Bryce Feng, Jun Pellitteri Hahn, Molly C. Scarlett, Cameron O. Li, Jiayi Feng, Yanbo Clark, Ryan L. Hefti, Hunter Schrope, Jonathan Venturelli, Ophelia S. Beebe, David J. Adv Sci (Weinh) Research Articles Oxygen levels in vivo are autonomously regulated by a supply–demand balance, which can be altered in disease states. However, the oxygen levels of in vitro cell culture systems, particularly microscale cell culture, are typically dominated by either supply or demand. Further, the oxygen microenvironment in these systems is rarely monitored or reported. Here, a method to establish and dynamically monitor autonomously regulated oxygen microenvironments (AROM) using an oil overlay in an open microscale cell culture system is presented. Using this method, the oxygen microenvironment is dynamically regulated via the supply–demand balance of the system. Numerical simulation and experimental validation of oxygen transport within multi‐liquid‐phase, microscale culture systems involving a variety of cell types, including mammalian, fungal, and bacterial cells are presented. Finally, AROM is applied to establish a coculture between cells with disparate oxygen demands—primary intestinal epithelial cells (oxygen consuming) and Bacteroides uniformis (an anaerobic species prevalent in the human gut). John Wiley and Sons Inc. 2022-02-04 /pmc/articles/PMC8981459/ /pubmed/35118834 http://dx.doi.org/10.1002/advs.202104510 Text en © 2022 The Authors. Advanced Science published by Wiley‐VCH GmbH https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Li, Chao
Humayun, Mouhita
Walker, Glenn M.
Park, Keon Young
Connors, Bryce
Feng, Jun
Pellitteri Hahn, Molly C.
Scarlett, Cameron O.
Li, Jiayi
Feng, Yanbo
Clark, Ryan L.
Hefti, Hunter
Schrope, Jonathan
Venturelli, Ophelia S.
Beebe, David J.
Under‐Oil Autonomously Regulated Oxygen Microenvironments: A Goldilocks Principle‐Based Approach for Microscale Cell Culture
title Under‐Oil Autonomously Regulated Oxygen Microenvironments: A Goldilocks Principle‐Based Approach for Microscale Cell Culture
title_full Under‐Oil Autonomously Regulated Oxygen Microenvironments: A Goldilocks Principle‐Based Approach for Microscale Cell Culture
title_fullStr Under‐Oil Autonomously Regulated Oxygen Microenvironments: A Goldilocks Principle‐Based Approach for Microscale Cell Culture
title_full_unstemmed Under‐Oil Autonomously Regulated Oxygen Microenvironments: A Goldilocks Principle‐Based Approach for Microscale Cell Culture
title_short Under‐Oil Autonomously Regulated Oxygen Microenvironments: A Goldilocks Principle‐Based Approach for Microscale Cell Culture
title_sort under‐oil autonomously regulated oxygen microenvironments: a goldilocks principle‐based approach for microscale cell culture
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8981459/
https://www.ncbi.nlm.nih.gov/pubmed/35118834
http://dx.doi.org/10.1002/advs.202104510
work_keys_str_mv AT lichao underoilautonomouslyregulatedoxygenmicroenvironmentsagoldilocksprinciplebasedapproachformicroscalecellculture
AT humayunmouhita underoilautonomouslyregulatedoxygenmicroenvironmentsagoldilocksprinciplebasedapproachformicroscalecellculture
AT walkerglennm underoilautonomouslyregulatedoxygenmicroenvironmentsagoldilocksprinciplebasedapproachformicroscalecellculture
AT parkkeonyoung underoilautonomouslyregulatedoxygenmicroenvironmentsagoldilocksprinciplebasedapproachformicroscalecellculture
AT connorsbryce underoilautonomouslyregulatedoxygenmicroenvironmentsagoldilocksprinciplebasedapproachformicroscalecellculture
AT fengjun underoilautonomouslyregulatedoxygenmicroenvironmentsagoldilocksprinciplebasedapproachformicroscalecellculture
AT pellitterihahnmollyc underoilautonomouslyregulatedoxygenmicroenvironmentsagoldilocksprinciplebasedapproachformicroscalecellculture
AT scarlettcamerono underoilautonomouslyregulatedoxygenmicroenvironmentsagoldilocksprinciplebasedapproachformicroscalecellculture
AT lijiayi underoilautonomouslyregulatedoxygenmicroenvironmentsagoldilocksprinciplebasedapproachformicroscalecellculture
AT fengyanbo underoilautonomouslyregulatedoxygenmicroenvironmentsagoldilocksprinciplebasedapproachformicroscalecellculture
AT clarkryanl underoilautonomouslyregulatedoxygenmicroenvironmentsagoldilocksprinciplebasedapproachformicroscalecellculture
AT heftihunter underoilautonomouslyregulatedoxygenmicroenvironmentsagoldilocksprinciplebasedapproachformicroscalecellculture
AT schropejonathan underoilautonomouslyregulatedoxygenmicroenvironmentsagoldilocksprinciplebasedapproachformicroscalecellculture
AT venturelliophelias underoilautonomouslyregulatedoxygenmicroenvironmentsagoldilocksprinciplebasedapproachformicroscalecellculture
AT beebedavidj underoilautonomouslyregulatedoxygenmicroenvironmentsagoldilocksprinciplebasedapproachformicroscalecellculture