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Under‐Oil Autonomously Regulated Oxygen Microenvironments: A Goldilocks Principle‐Based Approach for Microscale Cell Culture
Oxygen levels in vivo are autonomously regulated by a supply–demand balance, which can be altered in disease states. However, the oxygen levels of in vitro cell culture systems, particularly microscale cell culture, are typically dominated by either supply or demand. Further, the oxygen microenviron...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8981459/ https://www.ncbi.nlm.nih.gov/pubmed/35118834 http://dx.doi.org/10.1002/advs.202104510 |
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author | Li, Chao Humayun, Mouhita Walker, Glenn M. Park, Keon Young Connors, Bryce Feng, Jun Pellitteri Hahn, Molly C. Scarlett, Cameron O. Li, Jiayi Feng, Yanbo Clark, Ryan L. Hefti, Hunter Schrope, Jonathan Venturelli, Ophelia S. Beebe, David J. |
author_facet | Li, Chao Humayun, Mouhita Walker, Glenn M. Park, Keon Young Connors, Bryce Feng, Jun Pellitteri Hahn, Molly C. Scarlett, Cameron O. Li, Jiayi Feng, Yanbo Clark, Ryan L. Hefti, Hunter Schrope, Jonathan Venturelli, Ophelia S. Beebe, David J. |
author_sort | Li, Chao |
collection | PubMed |
description | Oxygen levels in vivo are autonomously regulated by a supply–demand balance, which can be altered in disease states. However, the oxygen levels of in vitro cell culture systems, particularly microscale cell culture, are typically dominated by either supply or demand. Further, the oxygen microenvironment in these systems is rarely monitored or reported. Here, a method to establish and dynamically monitor autonomously regulated oxygen microenvironments (AROM) using an oil overlay in an open microscale cell culture system is presented. Using this method, the oxygen microenvironment is dynamically regulated via the supply–demand balance of the system. Numerical simulation and experimental validation of oxygen transport within multi‐liquid‐phase, microscale culture systems involving a variety of cell types, including mammalian, fungal, and bacterial cells are presented. Finally, AROM is applied to establish a coculture between cells with disparate oxygen demands—primary intestinal epithelial cells (oxygen consuming) and Bacteroides uniformis (an anaerobic species prevalent in the human gut). |
format | Online Article Text |
id | pubmed-8981459 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-89814592022-04-11 Under‐Oil Autonomously Regulated Oxygen Microenvironments: A Goldilocks Principle‐Based Approach for Microscale Cell Culture Li, Chao Humayun, Mouhita Walker, Glenn M. Park, Keon Young Connors, Bryce Feng, Jun Pellitteri Hahn, Molly C. Scarlett, Cameron O. Li, Jiayi Feng, Yanbo Clark, Ryan L. Hefti, Hunter Schrope, Jonathan Venturelli, Ophelia S. Beebe, David J. Adv Sci (Weinh) Research Articles Oxygen levels in vivo are autonomously regulated by a supply–demand balance, which can be altered in disease states. However, the oxygen levels of in vitro cell culture systems, particularly microscale cell culture, are typically dominated by either supply or demand. Further, the oxygen microenvironment in these systems is rarely monitored or reported. Here, a method to establish and dynamically monitor autonomously regulated oxygen microenvironments (AROM) using an oil overlay in an open microscale cell culture system is presented. Using this method, the oxygen microenvironment is dynamically regulated via the supply–demand balance of the system. Numerical simulation and experimental validation of oxygen transport within multi‐liquid‐phase, microscale culture systems involving a variety of cell types, including mammalian, fungal, and bacterial cells are presented. Finally, AROM is applied to establish a coculture between cells with disparate oxygen demands—primary intestinal epithelial cells (oxygen consuming) and Bacteroides uniformis (an anaerobic species prevalent in the human gut). John Wiley and Sons Inc. 2022-02-04 /pmc/articles/PMC8981459/ /pubmed/35118834 http://dx.doi.org/10.1002/advs.202104510 Text en © 2022 The Authors. Advanced Science published by Wiley‐VCH GmbH https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Li, Chao Humayun, Mouhita Walker, Glenn M. Park, Keon Young Connors, Bryce Feng, Jun Pellitteri Hahn, Molly C. Scarlett, Cameron O. Li, Jiayi Feng, Yanbo Clark, Ryan L. Hefti, Hunter Schrope, Jonathan Venturelli, Ophelia S. Beebe, David J. Under‐Oil Autonomously Regulated Oxygen Microenvironments: A Goldilocks Principle‐Based Approach for Microscale Cell Culture |
title | Under‐Oil Autonomously Regulated Oxygen Microenvironments: A Goldilocks Principle‐Based Approach for Microscale Cell Culture |
title_full | Under‐Oil Autonomously Regulated Oxygen Microenvironments: A Goldilocks Principle‐Based Approach for Microscale Cell Culture |
title_fullStr | Under‐Oil Autonomously Regulated Oxygen Microenvironments: A Goldilocks Principle‐Based Approach for Microscale Cell Culture |
title_full_unstemmed | Under‐Oil Autonomously Regulated Oxygen Microenvironments: A Goldilocks Principle‐Based Approach for Microscale Cell Culture |
title_short | Under‐Oil Autonomously Regulated Oxygen Microenvironments: A Goldilocks Principle‐Based Approach for Microscale Cell Culture |
title_sort | under‐oil autonomously regulated oxygen microenvironments: a goldilocks principle‐based approach for microscale cell culture |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8981459/ https://www.ncbi.nlm.nih.gov/pubmed/35118834 http://dx.doi.org/10.1002/advs.202104510 |
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