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Functionalized Microgel Rods Interlinked into Soft Macroporous Structures for 3D Cell Culture

In this work, a two component microgel assembly using soft anisometric microgels that interlink to create a 3D macroporous construct for cell growth is reported. Reactive microgel rods with variable aspect ratio are produced via microfluidics in a continuous plug‐flow on‐chip gelation method by phot...

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Autores principales: Rommel, Dirk, Mork, Matthias, Vedaraman, Sitara, Bastard, Céline, Guerzoni, Luis P. B., Kittel, Yonca, Vinokur, Rostislav, Born, Nikolai, Haraszti, Tamás, De Laporte, Laura
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8981485/
https://www.ncbi.nlm.nih.gov/pubmed/35032119
http://dx.doi.org/10.1002/advs.202103554
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author Rommel, Dirk
Mork, Matthias
Vedaraman, Sitara
Bastard, Céline
Guerzoni, Luis P. B.
Kittel, Yonca
Vinokur, Rostislav
Born, Nikolai
Haraszti, Tamás
De Laporte, Laura
author_facet Rommel, Dirk
Mork, Matthias
Vedaraman, Sitara
Bastard, Céline
Guerzoni, Luis P. B.
Kittel, Yonca
Vinokur, Rostislav
Born, Nikolai
Haraszti, Tamás
De Laporte, Laura
author_sort Rommel, Dirk
collection PubMed
description In this work, a two component microgel assembly using soft anisometric microgels that interlink to create a 3D macroporous construct for cell growth is reported. Reactive microgel rods with variable aspect ratio are produced via microfluidics in a continuous plug‐flow on‐chip gelation method by photoinitiated free‐radical polymerization of star‐polyethylene glycol‐acrylate with glycidyl methacrylate or 2‐aminoethyl methacrylate comonomers. The resulting complementary epoxy‐ and amine‐functionalized microgels assemble and interlink with each other via a ring opening reaction, resulting in macroporous constructs with pores up to several hundreds of micrometers. The level of crosslinking depends on the functionalization degree of the microgels, which also affects the stiffness and cell adhesiveness of the microgels when modified with the cell‐adhesive GRGDS‐PC peptide. Therefore, 3D spreading and growth of cells inside the macroporous structure is influenced not only by the presence of macropores but also by the mechanical and biochemical properties of the individual microgels.
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spelling pubmed-89814852022-04-11 Functionalized Microgel Rods Interlinked into Soft Macroporous Structures for 3D Cell Culture Rommel, Dirk Mork, Matthias Vedaraman, Sitara Bastard, Céline Guerzoni, Luis P. B. Kittel, Yonca Vinokur, Rostislav Born, Nikolai Haraszti, Tamás De Laporte, Laura Adv Sci (Weinh) Research Articles In this work, a two component microgel assembly using soft anisometric microgels that interlink to create a 3D macroporous construct for cell growth is reported. Reactive microgel rods with variable aspect ratio are produced via microfluidics in a continuous plug‐flow on‐chip gelation method by photoinitiated free‐radical polymerization of star‐polyethylene glycol‐acrylate with glycidyl methacrylate or 2‐aminoethyl methacrylate comonomers. The resulting complementary epoxy‐ and amine‐functionalized microgels assemble and interlink with each other via a ring opening reaction, resulting in macroporous constructs with pores up to several hundreds of micrometers. The level of crosslinking depends on the functionalization degree of the microgels, which also affects the stiffness and cell adhesiveness of the microgels when modified with the cell‐adhesive GRGDS‐PC peptide. Therefore, 3D spreading and growth of cells inside the macroporous structure is influenced not only by the presence of macropores but also by the mechanical and biochemical properties of the individual microgels. John Wiley and Sons Inc. 2022-01-14 /pmc/articles/PMC8981485/ /pubmed/35032119 http://dx.doi.org/10.1002/advs.202103554 Text en © 2022 The Authors. Advanced Science published by Wiley‐VCH GmbH https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Rommel, Dirk
Mork, Matthias
Vedaraman, Sitara
Bastard, Céline
Guerzoni, Luis P. B.
Kittel, Yonca
Vinokur, Rostislav
Born, Nikolai
Haraszti, Tamás
De Laporte, Laura
Functionalized Microgel Rods Interlinked into Soft Macroporous Structures for 3D Cell Culture
title Functionalized Microgel Rods Interlinked into Soft Macroporous Structures for 3D Cell Culture
title_full Functionalized Microgel Rods Interlinked into Soft Macroporous Structures for 3D Cell Culture
title_fullStr Functionalized Microgel Rods Interlinked into Soft Macroporous Structures for 3D Cell Culture
title_full_unstemmed Functionalized Microgel Rods Interlinked into Soft Macroporous Structures for 3D Cell Culture
title_short Functionalized Microgel Rods Interlinked into Soft Macroporous Structures for 3D Cell Culture
title_sort functionalized microgel rods interlinked into soft macroporous structures for 3d cell culture
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8981485/
https://www.ncbi.nlm.nih.gov/pubmed/35032119
http://dx.doi.org/10.1002/advs.202103554
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