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A flexible kinetic assay efficiently sorts prospective biocatalysts for PET plastic subunit hydrolysis

Esterase enzymes catalyze diverse hydrolysis reactions with important biological, commercial, and biotechnological applications. For the improvement of these biocatalysts, there is a need for widely accessible, inexpensive, and adaptable activity screening assays that identify enzymes with particula...

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Detalles Bibliográficos
Autores principales: Lusty Beech, Jessica, Clare, Rita, Kincannon, William M., Erickson, Erika, McGeehan, John E., Beckham, Gregg T., DuBois, Jennifer L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8982334/
https://www.ncbi.nlm.nih.gov/pubmed/35424733
http://dx.doi.org/10.1039/d2ra00612j