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Genome size evolution in the beetle genus Diabrotica

Diabrocite corn rootworms are one of the most economically significant pests of maize in the United States and Europe and an emerging model for insect–plant interactions. Genome sizes of several species in the genus Diabrotica were estimated using flow cytometry along with that of Acalymma vittatum...

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Autores principales: Lata, Dimpal, Coates, Brad S, Walden, Kimberly K O, Robertson, Hugh M, Miller, Nicholas J
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8982398/
https://www.ncbi.nlm.nih.gov/pubmed/35234880
http://dx.doi.org/10.1093/g3journal/jkac052
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author Lata, Dimpal
Coates, Brad S
Walden, Kimberly K O
Robertson, Hugh M
Miller, Nicholas J
author_facet Lata, Dimpal
Coates, Brad S
Walden, Kimberly K O
Robertson, Hugh M
Miller, Nicholas J
author_sort Lata, Dimpal
collection PubMed
description Diabrocite corn rootworms are one of the most economically significant pests of maize in the United States and Europe and an emerging model for insect–plant interactions. Genome sizes of several species in the genus Diabrotica were estimated using flow cytometry along with that of Acalymma vittatum as an outgroup. Genome sizes ranged between 1.56 and 1.64 gigabase pairs and between 2.26 and 2.59 Gb, respectively, for the Diabrotica subgroups fucata and virgifera; the Acalymma vittatum genome size was around 1.65 Gb. This result indicated that a substantial increase in genome size occurred in the ancestor of the virgifera group. Further analysis of the fucata group and the virgifera group genome sequencing reads indicated that the genome size difference between the Diabrotica subgroups could be attributed to a higher content of transposable elements, mostly miniature inverted-transposable elements and gypsy-like long terminal repeat retroelements.
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spelling pubmed-89823982022-04-05 Genome size evolution in the beetle genus Diabrotica Lata, Dimpal Coates, Brad S Walden, Kimberly K O Robertson, Hugh M Miller, Nicholas J G3 (Bethesda) Investigation Diabrocite corn rootworms are one of the most economically significant pests of maize in the United States and Europe and an emerging model for insect–plant interactions. Genome sizes of several species in the genus Diabrotica were estimated using flow cytometry along with that of Acalymma vittatum as an outgroup. Genome sizes ranged between 1.56 and 1.64 gigabase pairs and between 2.26 and 2.59 Gb, respectively, for the Diabrotica subgroups fucata and virgifera; the Acalymma vittatum genome size was around 1.65 Gb. This result indicated that a substantial increase in genome size occurred in the ancestor of the virgifera group. Further analysis of the fucata group and the virgifera group genome sequencing reads indicated that the genome size difference between the Diabrotica subgroups could be attributed to a higher content of transposable elements, mostly miniature inverted-transposable elements and gypsy-like long terminal repeat retroelements. Oxford University Press 2022-03-02 /pmc/articles/PMC8982398/ /pubmed/35234880 http://dx.doi.org/10.1093/g3journal/jkac052 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of Genetics Society of America. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Investigation
Lata, Dimpal
Coates, Brad S
Walden, Kimberly K O
Robertson, Hugh M
Miller, Nicholas J
Genome size evolution in the beetle genus Diabrotica
title Genome size evolution in the beetle genus Diabrotica
title_full Genome size evolution in the beetle genus Diabrotica
title_fullStr Genome size evolution in the beetle genus Diabrotica
title_full_unstemmed Genome size evolution in the beetle genus Diabrotica
title_short Genome size evolution in the beetle genus Diabrotica
title_sort genome size evolution in the beetle genus diabrotica
topic Investigation
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8982398/
https://www.ncbi.nlm.nih.gov/pubmed/35234880
http://dx.doi.org/10.1093/g3journal/jkac052
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