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Establishment of Noninvasive Methods for the Detection of Helicobacter pylori in Mongolian Gerbils and Application of Main Laboratory Gerbil Populations in China

Identifying Helicobacter pylori (H. pylori, Hp) infection in animals before and after artificial infection influences the subsequent experiment. We established effective and noninvasive detection methods, including the gastric fluid nested polymerase chain reaction (PCR) method and the (13)C-urea br...

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Autores principales: Zhang, Xiulin, Wang, Cunlong, He, Yang, Xing, Jin, He, Yan, Huo, Xueyun, Fu, Rui, Lu, Xuancheng, Liu, Xin, Lv, Jianyi, Du, Xiaoyan, Chen, Zhenwen, Li, Changlong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8983185/
https://www.ncbi.nlm.nih.gov/pubmed/35392259
http://dx.doi.org/10.1155/2022/6036457
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author Zhang, Xiulin
Wang, Cunlong
He, Yang
Xing, Jin
He, Yan
Huo, Xueyun
Fu, Rui
Lu, Xuancheng
Liu, Xin
Lv, Jianyi
Du, Xiaoyan
Chen, Zhenwen
Li, Changlong
author_facet Zhang, Xiulin
Wang, Cunlong
He, Yang
Xing, Jin
He, Yan
Huo, Xueyun
Fu, Rui
Lu, Xuancheng
Liu, Xin
Lv, Jianyi
Du, Xiaoyan
Chen, Zhenwen
Li, Changlong
author_sort Zhang, Xiulin
collection PubMed
description Identifying Helicobacter pylori (H. pylori, Hp) infection in animals before and after artificial infection influences the subsequent experiment. We established effective and noninvasive detection methods, including the gastric fluid nested polymerase chain reaction (PCR) method and the (13)C-urea breath test, which can detect Hp before modeling Hp infection in Mongolian gerbils. We designed a gas collection equipment for gerbils. Hp nested PCR was also performed on gastric fluid, gastric mucosa, duodenal contents, and faeces of gerbils challenged with Hp. Conventional Hp detection methods, including rapid urease assay and immunohistochemistry, were compared. Moreover, we assessed the natural infection of Hp in 135 gerbils that had never been exposed to Hp artificially from the major laboratory gerbil groups in China. In 10 Hp infected gerbils, the positive detection results were 100%, 100%, 90%, and 10% in gastric fluid, gastric mucosa, duodenal contents, and faeces with nested PCR, respectively. A rapid urease test performed on gastric mucosa showed that all animals were infected with Hp. Immunohistochemical detection and bacteria culture of gastric mucosa samples that were positive by the nested PCR method also confirmed the presence of Hp. 9% (3/35) and 6% (2/31) natural infection rates were found in conventional gerbil groups from the Capital Medical University and Zhejiang Laboratory Animal Center. In conclusion, we established two noninvasive Hp detection methods that can be performed before modelingHp infection, including the gastric fluid nested PCR method and the (13)C-urea breath test.
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spelling pubmed-89831852022-04-06 Establishment of Noninvasive Methods for the Detection of Helicobacter pylori in Mongolian Gerbils and Application of Main Laboratory Gerbil Populations in China Zhang, Xiulin Wang, Cunlong He, Yang Xing, Jin He, Yan Huo, Xueyun Fu, Rui Lu, Xuancheng Liu, Xin Lv, Jianyi Du, Xiaoyan Chen, Zhenwen Li, Changlong Biomed Res Int Research Article Identifying Helicobacter pylori (H. pylori, Hp) infection in animals before and after artificial infection influences the subsequent experiment. We established effective and noninvasive detection methods, including the gastric fluid nested polymerase chain reaction (PCR) method and the (13)C-urea breath test, which can detect Hp before modeling Hp infection in Mongolian gerbils. We designed a gas collection equipment for gerbils. Hp nested PCR was also performed on gastric fluid, gastric mucosa, duodenal contents, and faeces of gerbils challenged with Hp. Conventional Hp detection methods, including rapid urease assay and immunohistochemistry, were compared. Moreover, we assessed the natural infection of Hp in 135 gerbils that had never been exposed to Hp artificially from the major laboratory gerbil groups in China. In 10 Hp infected gerbils, the positive detection results were 100%, 100%, 90%, and 10% in gastric fluid, gastric mucosa, duodenal contents, and faeces with nested PCR, respectively. A rapid urease test performed on gastric mucosa showed that all animals were infected with Hp. Immunohistochemical detection and bacteria culture of gastric mucosa samples that were positive by the nested PCR method also confirmed the presence of Hp. 9% (3/35) and 6% (2/31) natural infection rates were found in conventional gerbil groups from the Capital Medical University and Zhejiang Laboratory Animal Center. In conclusion, we established two noninvasive Hp detection methods that can be performed before modelingHp infection, including the gastric fluid nested PCR method and the (13)C-urea breath test. Hindawi 2022-03-29 /pmc/articles/PMC8983185/ /pubmed/35392259 http://dx.doi.org/10.1155/2022/6036457 Text en Copyright © 2022 Xiulin Zhang et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zhang, Xiulin
Wang, Cunlong
He, Yang
Xing, Jin
He, Yan
Huo, Xueyun
Fu, Rui
Lu, Xuancheng
Liu, Xin
Lv, Jianyi
Du, Xiaoyan
Chen, Zhenwen
Li, Changlong
Establishment of Noninvasive Methods for the Detection of Helicobacter pylori in Mongolian Gerbils and Application of Main Laboratory Gerbil Populations in China
title Establishment of Noninvasive Methods for the Detection of Helicobacter pylori in Mongolian Gerbils and Application of Main Laboratory Gerbil Populations in China
title_full Establishment of Noninvasive Methods for the Detection of Helicobacter pylori in Mongolian Gerbils and Application of Main Laboratory Gerbil Populations in China
title_fullStr Establishment of Noninvasive Methods for the Detection of Helicobacter pylori in Mongolian Gerbils and Application of Main Laboratory Gerbil Populations in China
title_full_unstemmed Establishment of Noninvasive Methods for the Detection of Helicobacter pylori in Mongolian Gerbils and Application of Main Laboratory Gerbil Populations in China
title_short Establishment of Noninvasive Methods for the Detection of Helicobacter pylori in Mongolian Gerbils and Application of Main Laboratory Gerbil Populations in China
title_sort establishment of noninvasive methods for the detection of helicobacter pylori in mongolian gerbils and application of main laboratory gerbil populations in china
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8983185/
https://www.ncbi.nlm.nih.gov/pubmed/35392259
http://dx.doi.org/10.1155/2022/6036457
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