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In Vitro and In Vivo Wound Healing Activity of Astragalus floccosus Boiss. (Fabaceae)

Estrogens are a group of sex hormones which have receptors on the skin and lead to increased cells and wound healing. Normally isoflavonoids are present in Astragalus floccosus Boiss. (Leguminosae). Therefore, the present study was conducted to evaluate the presence of isoflavonoids in A. floccosus&...

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Autores principales: Akbari, Fatemeh, Azadbakht, Mohammad, Bagheri, Ali, Vahedi, Lale
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8983193/
https://www.ncbi.nlm.nih.gov/pubmed/35392504
http://dx.doi.org/10.1155/2022/7865015
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author Akbari, Fatemeh
Azadbakht, Mohammad
Bagheri, Ali
Vahedi, Lale
author_facet Akbari, Fatemeh
Azadbakht, Mohammad
Bagheri, Ali
Vahedi, Lale
author_sort Akbari, Fatemeh
collection PubMed
description Estrogens are a group of sex hormones which have receptors on the skin and lead to increased cells and wound healing. Normally isoflavonoids are present in Astragalus floccosus Boiss. (Leguminosae). Therefore, the present study was conducted to evaluate the presence of isoflavonoids in A. floccosus' rich fraction of flavonoid and evaluate its wound healing effect accordingly. Flavonoids were evaluated by LCMS. Scratch was conducted and the medium culture was treated with the Astragalus' rich fraction of flavonoid (RFF) and was compared with nontreated culture during 48 hours. In addition, in vivo full-thickness wound healing evaluation was performed on rats. The rats were put into four groups and treated on a daily basis for 21 days with a cream containing 1.5% of the RFF (group 1), silver sulfadiazine (group 2), and Vaseline (group 3) separately. The nontreated group (group 4) was created for a better comparison. During the examination, wound size was evaluated and histopathological examination was performed. Herbal analysis detected 11 flavonoids, including 2 isoflavonoids, Calycosin-7-O-beta-D-glucoside and Formononetin, in the RFF. In vitro scratch wound healing showed significant improvement with RFF treatment in comparison to nontreated medium. Furthermore, in vitro drug release of Astragalus ointment showed a stationary line during 24 h and 0.14 mg/ml of flavonoid penetrated the skin. In vivo wound size evaluation showed significant improvement in the group treated with the RFF in comparison to other groups. Histopathological results indicated that congestion, edema, inflammation, necrosis, and angiogenesis decreased during the examination and fibroblast proliferation fibrosis epithelization was increased especially in the RFF group in comparison to the silver sulfadiazine and free groups. In conclusion, A. floccosus showed that wound healing activity in both in vitro and in vivo analyses can be attributed to the presence of isoflavonoids with estrogen-like activity in this plant.
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spelling pubmed-89831932022-04-06 In Vitro and In Vivo Wound Healing Activity of Astragalus floccosus Boiss. (Fabaceae) Akbari, Fatemeh Azadbakht, Mohammad Bagheri, Ali Vahedi, Lale Adv Pharmacol Pharm Sci Research Article Estrogens are a group of sex hormones which have receptors on the skin and lead to increased cells and wound healing. Normally isoflavonoids are present in Astragalus floccosus Boiss. (Leguminosae). Therefore, the present study was conducted to evaluate the presence of isoflavonoids in A. floccosus' rich fraction of flavonoid and evaluate its wound healing effect accordingly. Flavonoids were evaluated by LCMS. Scratch was conducted and the medium culture was treated with the Astragalus' rich fraction of flavonoid (RFF) and was compared with nontreated culture during 48 hours. In addition, in vivo full-thickness wound healing evaluation was performed on rats. The rats were put into four groups and treated on a daily basis for 21 days with a cream containing 1.5% of the RFF (group 1), silver sulfadiazine (group 2), and Vaseline (group 3) separately. The nontreated group (group 4) was created for a better comparison. During the examination, wound size was evaluated and histopathological examination was performed. Herbal analysis detected 11 flavonoids, including 2 isoflavonoids, Calycosin-7-O-beta-D-glucoside and Formononetin, in the RFF. In vitro scratch wound healing showed significant improvement with RFF treatment in comparison to nontreated medium. Furthermore, in vitro drug release of Astragalus ointment showed a stationary line during 24 h and 0.14 mg/ml of flavonoid penetrated the skin. In vivo wound size evaluation showed significant improvement in the group treated with the RFF in comparison to other groups. Histopathological results indicated that congestion, edema, inflammation, necrosis, and angiogenesis decreased during the examination and fibroblast proliferation fibrosis epithelization was increased especially in the RFF group in comparison to the silver sulfadiazine and free groups. In conclusion, A. floccosus showed that wound healing activity in both in vitro and in vivo analyses can be attributed to the presence of isoflavonoids with estrogen-like activity in this plant. Hindawi 2022-03-29 /pmc/articles/PMC8983193/ /pubmed/35392504 http://dx.doi.org/10.1155/2022/7865015 Text en Copyright © 2022 Fatemeh Akbari et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Akbari, Fatemeh
Azadbakht, Mohammad
Bagheri, Ali
Vahedi, Lale
In Vitro and In Vivo Wound Healing Activity of Astragalus floccosus Boiss. (Fabaceae)
title In Vitro and In Vivo Wound Healing Activity of Astragalus floccosus Boiss. (Fabaceae)
title_full In Vitro and In Vivo Wound Healing Activity of Astragalus floccosus Boiss. (Fabaceae)
title_fullStr In Vitro and In Vivo Wound Healing Activity of Astragalus floccosus Boiss. (Fabaceae)
title_full_unstemmed In Vitro and In Vivo Wound Healing Activity of Astragalus floccosus Boiss. (Fabaceae)
title_short In Vitro and In Vivo Wound Healing Activity of Astragalus floccosus Boiss. (Fabaceae)
title_sort in vitro and in vivo wound healing activity of astragalus floccosus boiss. (fabaceae)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8983193/
https://www.ncbi.nlm.nih.gov/pubmed/35392504
http://dx.doi.org/10.1155/2022/7865015
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