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Exosomal miR-543 Inhibits the Proliferation of Ovarian Cancer by Targeting IGF2

OBJECTIVE: Ovarian cancer (OvCa) is the most lethal gynaecological malignancy worldwide. We aimed to illustrate the potential function and molecular mechanism of exosomal microRNA-543 (miR-543) in the oncogenesis and development of OvCa. METHODS: Differentially expressed microRNAs in exosomes derive...

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Autores principales: Zhang, Shupei, Pan, Diling, Zhang, Shaoyu, Wu, Qiumei, Zhen, Lan, Liu, Shihuang, Chen, Jingjing, Lin, Rong, Hong, Qiuhua, Zheng, Xiangqin, Yi, Huan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8983272/
https://www.ncbi.nlm.nih.gov/pubmed/35391781
http://dx.doi.org/10.1155/2022/2003739
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author Zhang, Shupei
Pan, Diling
Zhang, Shaoyu
Wu, Qiumei
Zhen, Lan
Liu, Shihuang
Chen, Jingjing
Lin, Rong
Hong, Qiuhua
Zheng, Xiangqin
Yi, Huan
author_facet Zhang, Shupei
Pan, Diling
Zhang, Shaoyu
Wu, Qiumei
Zhen, Lan
Liu, Shihuang
Chen, Jingjing
Lin, Rong
Hong, Qiuhua
Zheng, Xiangqin
Yi, Huan
author_sort Zhang, Shupei
collection PubMed
description OBJECTIVE: Ovarian cancer (OvCa) is the most lethal gynaecological malignancy worldwide. We aimed to illustrate the potential function and molecular mechanism of exosomal microRNA-543 (miR-543) in the oncogenesis and development of OvCa. METHODS: Differentially expressed microRNAs in exosomes derived from OvCa cell lines were identified by bioinformatic analysis and verified by RT-PCR. Cell proliferation ability was estimated by clonogenic and 5-ethynyl-2′-deoxyuridine assays in vitro and in vivo. Potential involved pathways and targets of exosomal miRNAs were analysed using DIANA and verified by pyrosequencing, glucose quantification, dual-luciferase reporter experiments, and functional rescue assays. RESULTS: Bioinformatic analysis identified miR-543 and its potential target genes involved in the cancer-associated proteoglycan pathway. The expression of miR-543 was significantly decreased in exosomes derived from OvCa cell lines, patient serum, and OvCa tissues, while the mRNA levels of insulin-like growth factor 2 (IGF2) were increased. Furthermore, the overexpression of miR-543 resulted in the suppression of OvCa cell proliferation in vitro and in vivo. Moreover, miR-543 was significantly negatively correlated with IGF2 in OvCa tissues in comparison with paracarcinoma tissues. Notably, upregulation of miR-543 led to increased cell supernatant glucose levels and suppressed cell growth, which was rescued by overexpression of IGF2. CONCLUSIONS: Exosomal miR-543 participates in the proteoglycan pathway to suppress cell proliferation by targeting IGF2 in OvCa.
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spelling pubmed-89832722022-04-06 Exosomal miR-543 Inhibits the Proliferation of Ovarian Cancer by Targeting IGF2 Zhang, Shupei Pan, Diling Zhang, Shaoyu Wu, Qiumei Zhen, Lan Liu, Shihuang Chen, Jingjing Lin, Rong Hong, Qiuhua Zheng, Xiangqin Yi, Huan J Immunol Res Research Article OBJECTIVE: Ovarian cancer (OvCa) is the most lethal gynaecological malignancy worldwide. We aimed to illustrate the potential function and molecular mechanism of exosomal microRNA-543 (miR-543) in the oncogenesis and development of OvCa. METHODS: Differentially expressed microRNAs in exosomes derived from OvCa cell lines were identified by bioinformatic analysis and verified by RT-PCR. Cell proliferation ability was estimated by clonogenic and 5-ethynyl-2′-deoxyuridine assays in vitro and in vivo. Potential involved pathways and targets of exosomal miRNAs were analysed using DIANA and verified by pyrosequencing, glucose quantification, dual-luciferase reporter experiments, and functional rescue assays. RESULTS: Bioinformatic analysis identified miR-543 and its potential target genes involved in the cancer-associated proteoglycan pathway. The expression of miR-543 was significantly decreased in exosomes derived from OvCa cell lines, patient serum, and OvCa tissues, while the mRNA levels of insulin-like growth factor 2 (IGF2) were increased. Furthermore, the overexpression of miR-543 resulted in the suppression of OvCa cell proliferation in vitro and in vivo. Moreover, miR-543 was significantly negatively correlated with IGF2 in OvCa tissues in comparison with paracarcinoma tissues. Notably, upregulation of miR-543 led to increased cell supernatant glucose levels and suppressed cell growth, which was rescued by overexpression of IGF2. CONCLUSIONS: Exosomal miR-543 participates in the proteoglycan pathway to suppress cell proliferation by targeting IGF2 in OvCa. Hindawi 2022-03-29 /pmc/articles/PMC8983272/ /pubmed/35391781 http://dx.doi.org/10.1155/2022/2003739 Text en Copyright © 2022 Shupei Zhang et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zhang, Shupei
Pan, Diling
Zhang, Shaoyu
Wu, Qiumei
Zhen, Lan
Liu, Shihuang
Chen, Jingjing
Lin, Rong
Hong, Qiuhua
Zheng, Xiangqin
Yi, Huan
Exosomal miR-543 Inhibits the Proliferation of Ovarian Cancer by Targeting IGF2
title Exosomal miR-543 Inhibits the Proliferation of Ovarian Cancer by Targeting IGF2
title_full Exosomal miR-543 Inhibits the Proliferation of Ovarian Cancer by Targeting IGF2
title_fullStr Exosomal miR-543 Inhibits the Proliferation of Ovarian Cancer by Targeting IGF2
title_full_unstemmed Exosomal miR-543 Inhibits the Proliferation of Ovarian Cancer by Targeting IGF2
title_short Exosomal miR-543 Inhibits the Proliferation of Ovarian Cancer by Targeting IGF2
title_sort exosomal mir-543 inhibits the proliferation of ovarian cancer by targeting igf2
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8983272/
https://www.ncbi.nlm.nih.gov/pubmed/35391781
http://dx.doi.org/10.1155/2022/2003739
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