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Characterization of chicken IFI35 and its antiviral activity against Newcastle disease virus
Interferon-induced protein-35 kDa (IFI35) was an antiviral protein induced by interferon (IFN)-γ, which plays an important role in the IFN-mediated antiviral signaling pathway. Here, we cloned and identified IFI35 in the chicken for the first time. Chicken IFI35 (chIFI35) contains an open reading fr...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Japanese Society of Veterinary Science
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8983280/ https://www.ncbi.nlm.nih.gov/pubmed/35135934 http://dx.doi.org/10.1292/jvms.21-0410 |
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author | JIA, Yan Qing WANG, Xiang Wei CHEN, Xi QIU, Xin Xin WANG, Xing Long YANG, Zeng Qi |
author_facet | JIA, Yan Qing WANG, Xiang Wei CHEN, Xi QIU, Xin Xin WANG, Xing Long YANG, Zeng Qi |
author_sort | JIA, Yan Qing |
collection | PubMed |
description | Interferon-induced protein-35 kDa (IFI35) was an antiviral protein induced by interferon (IFN)-γ, which plays an important role in the IFN-mediated antiviral signaling pathway. Here, we cloned and identified IFI35 in the chicken for the first time. Chicken IFI35 (chIFI35) contains an open reading frame (ORF) of 1,152 bp encoding a protein of 384 amino acids containing two conserved Nmi/IFI35 domain (NID) motifs. Tissue distribution analysis of chIFI35 in healthy and Newcastle disease (ND) virus-infected chickens indicated a positive correlation between chIFI35 mRNA transcription and ND viral loads in various tissues. The role of chIFI35 in regulation NDV replication were further assessed by up- or down-regulated chIFI35 expression in DF-1 cells transfected with plasmid harboring chIFI35, pCMV-3HA-chIFI35 or shRNA targeting chIFI35, pshRNA-chIFI35 plasmids. NDV replications in DF-1 cells were significantly reduced or slightly increased by over- or under-expression of the chIFI35 protein, respectively, indicating the role of chIFI35 in anti-NDV infection. Moreover, chIFI35 also involved in regulation of viral gene transcription and IFNs expression. The collected data were meaningful for research of chicken antiviral immunity and shed light on the pleiotropic antiviral effect of chIFI35 during NDV infection. |
format | Online Article Text |
id | pubmed-8983280 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | The Japanese Society of Veterinary Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-89832802022-04-14 Characterization of chicken IFI35 and its antiviral activity against Newcastle disease virus JIA, Yan Qing WANG, Xiang Wei CHEN, Xi QIU, Xin Xin WANG, Xing Long YANG, Zeng Qi J Vet Med Sci Virology Interferon-induced protein-35 kDa (IFI35) was an antiviral protein induced by interferon (IFN)-γ, which plays an important role in the IFN-mediated antiviral signaling pathway. Here, we cloned and identified IFI35 in the chicken for the first time. Chicken IFI35 (chIFI35) contains an open reading frame (ORF) of 1,152 bp encoding a protein of 384 amino acids containing two conserved Nmi/IFI35 domain (NID) motifs. Tissue distribution analysis of chIFI35 in healthy and Newcastle disease (ND) virus-infected chickens indicated a positive correlation between chIFI35 mRNA transcription and ND viral loads in various tissues. The role of chIFI35 in regulation NDV replication were further assessed by up- or down-regulated chIFI35 expression in DF-1 cells transfected with plasmid harboring chIFI35, pCMV-3HA-chIFI35 or shRNA targeting chIFI35, pshRNA-chIFI35 plasmids. NDV replications in DF-1 cells were significantly reduced or slightly increased by over- or under-expression of the chIFI35 protein, respectively, indicating the role of chIFI35 in anti-NDV infection. Moreover, chIFI35 also involved in regulation of viral gene transcription and IFNs expression. The collected data were meaningful for research of chicken antiviral immunity and shed light on the pleiotropic antiviral effect of chIFI35 during NDV infection. The Japanese Society of Veterinary Science 2022-02-09 2022-03 /pmc/articles/PMC8983280/ /pubmed/35135934 http://dx.doi.org/10.1292/jvms.21-0410 Text en ©2022 The Japanese Society of Veterinary Science https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/) |
spellingShingle | Virology JIA, Yan Qing WANG, Xiang Wei CHEN, Xi QIU, Xin Xin WANG, Xing Long YANG, Zeng Qi Characterization of chicken IFI35 and its antiviral activity against Newcastle disease virus |
title | Characterization of chicken IFI35 and its antiviral activity against Newcastle disease virus |
title_full | Characterization of chicken IFI35 and its antiviral activity against Newcastle disease virus |
title_fullStr | Characterization of chicken IFI35 and its antiviral activity against Newcastle disease virus |
title_full_unstemmed | Characterization of chicken IFI35 and its antiviral activity against Newcastle disease virus |
title_short | Characterization of chicken IFI35 and its antiviral activity against Newcastle disease virus |
title_sort | characterization of chicken ifi35 and its antiviral activity against newcastle disease virus |
topic | Virology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8983280/ https://www.ncbi.nlm.nih.gov/pubmed/35135934 http://dx.doi.org/10.1292/jvms.21-0410 |
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