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Characterization of chicken IFI35 and its antiviral activity against Newcastle disease virus

Interferon-induced protein-35 kDa (IFI35) was an antiviral protein induced by interferon (IFN)-γ, which plays an important role in the IFN-mediated antiviral signaling pathway. Here, we cloned and identified IFI35 in the chicken for the first time. Chicken IFI35 (chIFI35) contains an open reading fr...

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Autores principales: JIA, Yan Qing, WANG, Xiang Wei, CHEN, Xi, QIU, Xin Xin, WANG, Xing Long, YANG, Zeng Qi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8983280/
https://www.ncbi.nlm.nih.gov/pubmed/35135934
http://dx.doi.org/10.1292/jvms.21-0410
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author JIA, Yan Qing
WANG, Xiang Wei
CHEN, Xi
QIU, Xin Xin
WANG, Xing Long
YANG, Zeng Qi
author_facet JIA, Yan Qing
WANG, Xiang Wei
CHEN, Xi
QIU, Xin Xin
WANG, Xing Long
YANG, Zeng Qi
author_sort JIA, Yan Qing
collection PubMed
description Interferon-induced protein-35 kDa (IFI35) was an antiviral protein induced by interferon (IFN)-γ, which plays an important role in the IFN-mediated antiviral signaling pathway. Here, we cloned and identified IFI35 in the chicken for the first time. Chicken IFI35 (chIFI35) contains an open reading frame (ORF) of 1,152 bp encoding a protein of 384 amino acids containing two conserved Nmi/IFI35 domain (NID) motifs. Tissue distribution analysis of chIFI35 in healthy and Newcastle disease (ND) virus-infected chickens indicated a positive correlation between chIFI35 mRNA transcription and ND viral loads in various tissues. The role of chIFI35 in regulation NDV replication were further assessed by up- or down-regulated chIFI35 expression in DF-1 cells transfected with plasmid harboring chIFI35, pCMV-3HA-chIFI35 or shRNA targeting chIFI35, pshRNA-chIFI35 plasmids. NDV replications in DF-1 cells were significantly reduced or slightly increased by over- or under-expression of the chIFI35 protein, respectively, indicating the role of chIFI35 in anti-NDV infection. Moreover, chIFI35 also involved in regulation of viral gene transcription and IFNs expression. The collected data were meaningful for research of chicken antiviral immunity and shed light on the pleiotropic antiviral effect of chIFI35 during NDV infection.
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spelling pubmed-89832802022-04-14 Characterization of chicken IFI35 and its antiviral activity against Newcastle disease virus JIA, Yan Qing WANG, Xiang Wei CHEN, Xi QIU, Xin Xin WANG, Xing Long YANG, Zeng Qi J Vet Med Sci Virology Interferon-induced protein-35 kDa (IFI35) was an antiviral protein induced by interferon (IFN)-γ, which plays an important role in the IFN-mediated antiviral signaling pathway. Here, we cloned and identified IFI35 in the chicken for the first time. Chicken IFI35 (chIFI35) contains an open reading frame (ORF) of 1,152 bp encoding a protein of 384 amino acids containing two conserved Nmi/IFI35 domain (NID) motifs. Tissue distribution analysis of chIFI35 in healthy and Newcastle disease (ND) virus-infected chickens indicated a positive correlation between chIFI35 mRNA transcription and ND viral loads in various tissues. The role of chIFI35 in regulation NDV replication were further assessed by up- or down-regulated chIFI35 expression in DF-1 cells transfected with plasmid harboring chIFI35, pCMV-3HA-chIFI35 or shRNA targeting chIFI35, pshRNA-chIFI35 plasmids. NDV replications in DF-1 cells were significantly reduced or slightly increased by over- or under-expression of the chIFI35 protein, respectively, indicating the role of chIFI35 in anti-NDV infection. Moreover, chIFI35 also involved in regulation of viral gene transcription and IFNs expression. The collected data were meaningful for research of chicken antiviral immunity and shed light on the pleiotropic antiviral effect of chIFI35 during NDV infection. The Japanese Society of Veterinary Science 2022-02-09 2022-03 /pmc/articles/PMC8983280/ /pubmed/35135934 http://dx.doi.org/10.1292/jvms.21-0410 Text en ©2022 The Japanese Society of Veterinary Science https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Virology
JIA, Yan Qing
WANG, Xiang Wei
CHEN, Xi
QIU, Xin Xin
WANG, Xing Long
YANG, Zeng Qi
Characterization of chicken IFI35 and its antiviral activity against Newcastle disease virus
title Characterization of chicken IFI35 and its antiviral activity against Newcastle disease virus
title_full Characterization of chicken IFI35 and its antiviral activity against Newcastle disease virus
title_fullStr Characterization of chicken IFI35 and its antiviral activity against Newcastle disease virus
title_full_unstemmed Characterization of chicken IFI35 and its antiviral activity against Newcastle disease virus
title_short Characterization of chicken IFI35 and its antiviral activity against Newcastle disease virus
title_sort characterization of chicken ifi35 and its antiviral activity against newcastle disease virus
topic Virology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8983280/
https://www.ncbi.nlm.nih.gov/pubmed/35135934
http://dx.doi.org/10.1292/jvms.21-0410
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