Identification and phylogenetic analysis of Babesia parasites in domestic dogs in Nigeria

The present study examined the presence of Babesia parasites in 104 domestic dogs in Nigeria. Sequentially, Babesia parasites infecting domestic dogs underwent genetic and phylogenetic analyses. The results of nested PCR based on the Piroplasmida 18S rRNA gene illustrated that 13.5% (14/104) of the...

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Autores principales: HIRATA, Haruyuki, OMOBOWALE, Temidayo, ADEBAYO, Olufunke, ASANUMA, Nodoka, HARAGUCHI, Asako, MURAKAMI, Yoshiki, KUSAKISAKO, Kodai, IKEDA, Keiko, ASAKAWA, Mitsuhiko, SUZUKI, Kazuyuki, ISHIHARA, Chiaki, IKADAI, Hiromi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2022
Materias:
Parasitology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8983282/
https://www.ncbi.nlm.nih.gov/pubmed/35110460
http://dx.doi.org/10.1292/jvms.21-0636
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author HIRATA, Haruyuki
OMOBOWALE, Temidayo
ADEBAYO, Olufunke
ASANUMA, Nodoka
HARAGUCHI, Asako
MURAKAMI, Yoshiki
KUSAKISAKO, Kodai
IKEDA, Keiko
ASAKAWA, Mitsuhiko
SUZUKI, Kazuyuki
ISHIHARA, Chiaki
IKADAI, Hiromi
author_facet HIRATA, Haruyuki
OMOBOWALE, Temidayo
ADEBAYO, Olufunke
ASANUMA, Nodoka
HARAGUCHI, Asako
MURAKAMI, Yoshiki
KUSAKISAKO, Kodai
IKEDA, Keiko
ASAKAWA, Mitsuhiko
SUZUKI, Kazuyuki
ISHIHARA, Chiaki
IKADAI, Hiromi
author_sort HIRATA, Haruyuki
collection PubMed
description The present study examined the presence of Babesia parasites in 104 domestic dogs in Nigeria. Sequentially, Babesia parasites infecting domestic dogs underwent genetic and phylogenetic analyses. The results of nested PCR based on the Piroplasmida 18S rRNA gene illustrated that 13.5% (14/104) of the samples were positive. The obtained positive samples determined the nucleotide sequences of the 18S rRNA genes. In the genetic and phylogenetic analyses, four of five nucleotide sequences were similar to Babesia canis rossi, and one sample exhibited a close similarity to a Babesia sp. isolated from a raccoon in Hokkaido, Japan. The present study revealed the widespread presence of B. canis rossi among domestic dogs in Nigeria.
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spelling pubmed-89832822022-04-14 Identification and phylogenetic analysis of Babesia parasites in domestic dogs in Nigeria HIRATA, Haruyuki OMOBOWALE, Temidayo ADEBAYO, Olufunke ASANUMA, Nodoka HARAGUCHI, Asako MURAKAMI, Yoshiki KUSAKISAKO, Kodai IKEDA, Keiko ASAKAWA, Mitsuhiko SUZUKI, Kazuyuki ISHIHARA, Chiaki IKADAI, Hiromi J Vet Med Sci Parasitology The present study examined the presence of Babesia parasites in 104 domestic dogs in Nigeria. Sequentially, Babesia parasites infecting domestic dogs underwent genetic and phylogenetic analyses. The results of nested PCR based on the Piroplasmida 18S rRNA gene illustrated that 13.5% (14/104) of the samples were positive. The obtained positive samples determined the nucleotide sequences of the 18S rRNA genes. In the genetic and phylogenetic analyses, four of five nucleotide sequences were similar to Babesia canis rossi, and one sample exhibited a close similarity to a Babesia sp. isolated from a raccoon in Hokkaido, Japan. The present study revealed the widespread presence of B. canis rossi among domestic dogs in Nigeria. The Japanese Society of Veterinary Science 2022-02-02 2022-03 /pmc/articles/PMC8983282/ /pubmed/35110460 http://dx.doi.org/10.1292/jvms.21-0636 Text en ©2022 The Japanese Society of Veterinary Science https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Parasitology
HIRATA, Haruyuki
OMOBOWALE, Temidayo
ADEBAYO, Olufunke
ASANUMA, Nodoka
HARAGUCHI, Asako
MURAKAMI, Yoshiki
KUSAKISAKO, Kodai
IKEDA, Keiko
ASAKAWA, Mitsuhiko
SUZUKI, Kazuyuki
ISHIHARA, Chiaki
IKADAI, Hiromi
Identification and phylogenetic analysis of Babesia parasites in domestic dogs in Nigeria
title Identification and phylogenetic analysis of Babesia parasites in domestic dogs in Nigeria
title_full Identification and phylogenetic analysis of Babesia parasites in domestic dogs in Nigeria
title_fullStr Identification and phylogenetic analysis of Babesia parasites in domestic dogs in Nigeria
title_full_unstemmed Identification and phylogenetic analysis of Babesia parasites in domestic dogs in Nigeria
title_short Identification and phylogenetic analysis of Babesia parasites in domestic dogs in Nigeria
title_sort identification and phylogenetic analysis of babesia parasites in domestic dogs in nigeria
topic Parasitology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8983282/
https://www.ncbi.nlm.nih.gov/pubmed/35110460
http://dx.doi.org/10.1292/jvms.21-0636
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