Identification of the interacting partners of a lysosomal membrane protein in living cells by BioID technique

The purpose of this protocol is to screen and identify the physiologically relevant interactors of a lysosomal protein in living cells. Here, we describe how to identify solute carrier family 15 member 4 (SLC15A4)-interacting proteins by BioID and mass spectrometry analysis. This protocol utilizes f...

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Detalles Bibliográficos
Autores principales: Nguyen-Tien, Dat, Suzuki, Takehiro, Kobayashi, Toshihiko, Toyama-Sorimachi, Noriko, Dohmae, Naoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8983420/
https://www.ncbi.nlm.nih.gov/pubmed/35403001
http://dx.doi.org/10.1016/j.xpro.2022.101263

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