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A Self-Reference Interference Sensor Based on Coherence Multiplexing

Interferometry has been widely used in biosensing due to its ability to acquire molecular affinity and kinetics in real-time. However, interferometric-based sensors are susceptible to environmental disturbances, including temperature and non-specific binding of target molecules, which reduces their...

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Autores principales: Shen, Ying, Huang, Zeyu, Huang, Feng, He, Yonghong, Ye, Ziling, Zhang, Hongjian, Guo, Cuixia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8983844/
https://www.ncbi.nlm.nih.gov/pubmed/35402379
http://dx.doi.org/10.3389/fchem.2022.880081
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author Shen, Ying
Huang, Zeyu
Huang, Feng
He, Yonghong
Ye, Ziling
Zhang, Hongjian
Guo, Cuixia
author_facet Shen, Ying
Huang, Zeyu
Huang, Feng
He, Yonghong
Ye, Ziling
Zhang, Hongjian
Guo, Cuixia
author_sort Shen, Ying
collection PubMed
description Interferometry has been widely used in biosensing due to its ability to acquire molecular affinity and kinetics in real-time. However, interferometric-based sensors are susceptible to environmental disturbances, including temperature and non-specific binding of target molecules, which reduces their detection robustness. To address this shortcoming, this paper proposes a self-referencing interference sensor based on coherence multiplexing to resist environmental disturbances. The proposed sensor can address temperature and non-specific binding, but it is not limited only to these types of disturbances. In the proposed sensor design, each sensor signal is encoded using a specific optical path difference determined by the optical thickness of a sensor chip. In addition, two sensor signals for disturbances tracking and biomolecule detection are detected simultaneously without additional cost to the second spectrometer and then differenced to achieve real-time self-reference. The temperature fluctuations experiments and specific binding experiments of protein A to IgG are performed to verify the performance of the proposed sensor. The results demonstrate that the proposed sensor can eliminate non-specific binding and temperature disturbances in real-time during biomolecule detection, achieving higher detection robustness. The proposed sensor is suitable for applications that require large-scale testing of biomolecular interactions, such as drug screening.
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spelling pubmed-89838442022-04-07 A Self-Reference Interference Sensor Based on Coherence Multiplexing Shen, Ying Huang, Zeyu Huang, Feng He, Yonghong Ye, Ziling Zhang, Hongjian Guo, Cuixia Front Chem Chemistry Interferometry has been widely used in biosensing due to its ability to acquire molecular affinity and kinetics in real-time. However, interferometric-based sensors are susceptible to environmental disturbances, including temperature and non-specific binding of target molecules, which reduces their detection robustness. To address this shortcoming, this paper proposes a self-referencing interference sensor based on coherence multiplexing to resist environmental disturbances. The proposed sensor can address temperature and non-specific binding, but it is not limited only to these types of disturbances. In the proposed sensor design, each sensor signal is encoded using a specific optical path difference determined by the optical thickness of a sensor chip. In addition, two sensor signals for disturbances tracking and biomolecule detection are detected simultaneously without additional cost to the second spectrometer and then differenced to achieve real-time self-reference. The temperature fluctuations experiments and specific binding experiments of protein A to IgG are performed to verify the performance of the proposed sensor. The results demonstrate that the proposed sensor can eliminate non-specific binding and temperature disturbances in real-time during biomolecule detection, achieving higher detection robustness. The proposed sensor is suitable for applications that require large-scale testing of biomolecular interactions, such as drug screening. Frontiers Media S.A. 2022-03-23 /pmc/articles/PMC8983844/ /pubmed/35402379 http://dx.doi.org/10.3389/fchem.2022.880081 Text en Copyright © 2022 Shen, Huang, Huang, He, Ye, Zhang and Guo. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Chemistry
Shen, Ying
Huang, Zeyu
Huang, Feng
He, Yonghong
Ye, Ziling
Zhang, Hongjian
Guo, Cuixia
A Self-Reference Interference Sensor Based on Coherence Multiplexing
title A Self-Reference Interference Sensor Based on Coherence Multiplexing
title_full A Self-Reference Interference Sensor Based on Coherence Multiplexing
title_fullStr A Self-Reference Interference Sensor Based on Coherence Multiplexing
title_full_unstemmed A Self-Reference Interference Sensor Based on Coherence Multiplexing
title_short A Self-Reference Interference Sensor Based on Coherence Multiplexing
title_sort self-reference interference sensor based on coherence multiplexing
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8983844/
https://www.ncbi.nlm.nih.gov/pubmed/35402379
http://dx.doi.org/10.3389/fchem.2022.880081
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