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Suppression of SDP1 Improves Soybean Seed Composition by Increasing Oil and Reducing Undigestible Oligosaccharides

In developing soybean seeds, carbon is partitioned between oil, protein and carbohydrates. Here, we demonstrate that suppression of lipase-mediated turnover of triacylglycerols (TAG) during late seed development increases fatty acid content and decreases the presence of undigestible oligosaccharides...

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Autores principales: Aznar-Moreno, Jose A., Mukherjee, Thiya, Morley, Stewart A., Duressa, Dechassa, Kambhampati, Shrikaar, Chu, Kevin L., Koley, Somnath, Allen, Doug K., Durrett, Timothy P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8983916/
https://www.ncbi.nlm.nih.gov/pubmed/35401590
http://dx.doi.org/10.3389/fpls.2022.863254
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author Aznar-Moreno, Jose A.
Mukherjee, Thiya
Morley, Stewart A.
Duressa, Dechassa
Kambhampati, Shrikaar
Chu, Kevin L.
Koley, Somnath
Allen, Doug K.
Durrett, Timothy P.
author_facet Aznar-Moreno, Jose A.
Mukherjee, Thiya
Morley, Stewart A.
Duressa, Dechassa
Kambhampati, Shrikaar
Chu, Kevin L.
Koley, Somnath
Allen, Doug K.
Durrett, Timothy P.
author_sort Aznar-Moreno, Jose A.
collection PubMed
description In developing soybean seeds, carbon is partitioned between oil, protein and carbohydrates. Here, we demonstrate that suppression of lipase-mediated turnover of triacylglycerols (TAG) during late seed development increases fatty acid content and decreases the presence of undigestible oligosaccharides. During late stages of embryo development, the fatty acid content of soybean seed decreases while the levels of the oligosaccharides raffinose and stachyose increase. Three soybean genes orthologous to the Arabidopsis lipase gene SUGAR-DEPENDENT1 (SDP1) are upregulated at this time. Suppression of these genes resulted in higher oil levels, with lipid levels in the best lines exceeding 24% of seed weight. In addition, lipase-suppressed lines produced larger seeds compared to wild-type plants, resulting in increases of over 20% in total lipid per seed. Levels of raffinose and stachyose were lower in the transgenic lines, with average reductions of 15% in total raffinose family oligosaccharides observed. Despite the increase in oil, protein content was not negatively impacted and trended higher in the transgenic lines. These results are consistent with a role for SDP1 in turning over TAG to supply carbon for other needs, including the synthesis of oligosaccharides, and offer new strategies to further improve the composition of soybean seeds.
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spelling pubmed-89839162022-04-07 Suppression of SDP1 Improves Soybean Seed Composition by Increasing Oil and Reducing Undigestible Oligosaccharides Aznar-Moreno, Jose A. Mukherjee, Thiya Morley, Stewart A. Duressa, Dechassa Kambhampati, Shrikaar Chu, Kevin L. Koley, Somnath Allen, Doug K. Durrett, Timothy P. Front Plant Sci Plant Science In developing soybean seeds, carbon is partitioned between oil, protein and carbohydrates. Here, we demonstrate that suppression of lipase-mediated turnover of triacylglycerols (TAG) during late seed development increases fatty acid content and decreases the presence of undigestible oligosaccharides. During late stages of embryo development, the fatty acid content of soybean seed decreases while the levels of the oligosaccharides raffinose and stachyose increase. Three soybean genes orthologous to the Arabidopsis lipase gene SUGAR-DEPENDENT1 (SDP1) are upregulated at this time. Suppression of these genes resulted in higher oil levels, with lipid levels in the best lines exceeding 24% of seed weight. In addition, lipase-suppressed lines produced larger seeds compared to wild-type plants, resulting in increases of over 20% in total lipid per seed. Levels of raffinose and stachyose were lower in the transgenic lines, with average reductions of 15% in total raffinose family oligosaccharides observed. Despite the increase in oil, protein content was not negatively impacted and trended higher in the transgenic lines. These results are consistent with a role for SDP1 in turning over TAG to supply carbon for other needs, including the synthesis of oligosaccharides, and offer new strategies to further improve the composition of soybean seeds. Frontiers Media S.A. 2022-03-23 /pmc/articles/PMC8983916/ /pubmed/35401590 http://dx.doi.org/10.3389/fpls.2022.863254 Text en Copyright © 2022 Aznar-Moreno, Mukherjee, Morley, Duressa, Kambhampati, Chu, Koley, Allen and Durrett. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Aznar-Moreno, Jose A.
Mukherjee, Thiya
Morley, Stewart A.
Duressa, Dechassa
Kambhampati, Shrikaar
Chu, Kevin L.
Koley, Somnath
Allen, Doug K.
Durrett, Timothy P.
Suppression of SDP1 Improves Soybean Seed Composition by Increasing Oil and Reducing Undigestible Oligosaccharides
title Suppression of SDP1 Improves Soybean Seed Composition by Increasing Oil and Reducing Undigestible Oligosaccharides
title_full Suppression of SDP1 Improves Soybean Seed Composition by Increasing Oil and Reducing Undigestible Oligosaccharides
title_fullStr Suppression of SDP1 Improves Soybean Seed Composition by Increasing Oil and Reducing Undigestible Oligosaccharides
title_full_unstemmed Suppression of SDP1 Improves Soybean Seed Composition by Increasing Oil and Reducing Undigestible Oligosaccharides
title_short Suppression of SDP1 Improves Soybean Seed Composition by Increasing Oil and Reducing Undigestible Oligosaccharides
title_sort suppression of sdp1 improves soybean seed composition by increasing oil and reducing undigestible oligosaccharides
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8983916/
https://www.ncbi.nlm.nih.gov/pubmed/35401590
http://dx.doi.org/10.3389/fpls.2022.863254
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