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Elevated METTL9 is associated with peritoneal dissemination in human scirrhous gastric cancers
Methylation, the most common chemical modification of cellular components such as DNA, RNA, and proteins, impacts biological processes including transcription, RNA processing, and protein dynamics. Although abnormal expression of methyltransferase can lead to various diseases including cancers, litt...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8983939/ https://www.ncbi.nlm.nih.gov/pubmed/35402738 http://dx.doi.org/10.1016/j.bbrep.2022.101255 |
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author | Hara, Toshifumi Tominaga, Yuuki Ueda, Koji Mihara, Keichiro Yanagihara, Kazuyoshi Takei, Yoshifumi |
author_facet | Hara, Toshifumi Tominaga, Yuuki Ueda, Koji Mihara, Keichiro Yanagihara, Kazuyoshi Takei, Yoshifumi |
author_sort | Hara, Toshifumi |
collection | PubMed |
description | Methylation, the most common chemical modification of cellular components such as DNA, RNA, and proteins, impacts biological processes including transcription, RNA processing, and protein dynamics. Although abnormal expression of methyltransferase can lead to various diseases including cancers, little is known about the relationship between methyltransferase and cancers. Here we aimed to understand the role of methyltransferase in cancer metastasis. We found that elevated methyltransferase-like 9 (METTL9) is closely associated with the acquisition of metastatic activity in human scirrhous gastric cancers. The stable knockdown of METTL9 via an shRNA vector technique in our original metastatic cells from scirrhous gastric cancer patients significantly inhibited migration and invasion. In metastatic cells, METTL9 protein is predominantly localized in mitochondria, and the METTL9 knockdown significantly reduced mitochondrial Complex I activity. METTL9 can be a candidate of molecular targets to inhibit peritoneal dissemination of scirrhous gastric cancers. This report is the first to describe the relationship between METTL9 and cancer metastasis. |
format | Online Article Text |
id | pubmed-8983939 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-89839392022-04-07 Elevated METTL9 is associated with peritoneal dissemination in human scirrhous gastric cancers Hara, Toshifumi Tominaga, Yuuki Ueda, Koji Mihara, Keichiro Yanagihara, Kazuyoshi Takei, Yoshifumi Biochem Biophys Rep Research Article Methylation, the most common chemical modification of cellular components such as DNA, RNA, and proteins, impacts biological processes including transcription, RNA processing, and protein dynamics. Although abnormal expression of methyltransferase can lead to various diseases including cancers, little is known about the relationship between methyltransferase and cancers. Here we aimed to understand the role of methyltransferase in cancer metastasis. We found that elevated methyltransferase-like 9 (METTL9) is closely associated with the acquisition of metastatic activity in human scirrhous gastric cancers. The stable knockdown of METTL9 via an shRNA vector technique in our original metastatic cells from scirrhous gastric cancer patients significantly inhibited migration and invasion. In metastatic cells, METTL9 protein is predominantly localized in mitochondria, and the METTL9 knockdown significantly reduced mitochondrial Complex I activity. METTL9 can be a candidate of molecular targets to inhibit peritoneal dissemination of scirrhous gastric cancers. This report is the first to describe the relationship between METTL9 and cancer metastasis. Elsevier 2022-04-02 /pmc/articles/PMC8983939/ /pubmed/35402738 http://dx.doi.org/10.1016/j.bbrep.2022.101255 Text en © 2022 The Authors. Published by Elsevier B.V. https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Research Article Hara, Toshifumi Tominaga, Yuuki Ueda, Koji Mihara, Keichiro Yanagihara, Kazuyoshi Takei, Yoshifumi Elevated METTL9 is associated with peritoneal dissemination in human scirrhous gastric cancers |
title | Elevated METTL9 is associated with peritoneal dissemination in human scirrhous gastric cancers |
title_full | Elevated METTL9 is associated with peritoneal dissemination in human scirrhous gastric cancers |
title_fullStr | Elevated METTL9 is associated with peritoneal dissemination in human scirrhous gastric cancers |
title_full_unstemmed | Elevated METTL9 is associated with peritoneal dissemination in human scirrhous gastric cancers |
title_short | Elevated METTL9 is associated with peritoneal dissemination in human scirrhous gastric cancers |
title_sort | elevated mettl9 is associated with peritoneal dissemination in human scirrhous gastric cancers |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8983939/ https://www.ncbi.nlm.nih.gov/pubmed/35402738 http://dx.doi.org/10.1016/j.bbrep.2022.101255 |
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