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The Long Non-Coding RNA HOXC-AS3 Promotes Glioma Progression by Sponging miR-216 to Regulate F11R Expression

HOXC cluster antisense RNA 3 (HOXC-AS3) is a long noncoding RNA (lncRNA) that plays a crucial role in various tumors; nevertheless, its role in glioma and its mechanism have not been completely elucidated. In this research, we discovered that HOXC-AS3 was over-expression in glioma cells and tissues...

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Autores principales: Li, Yongshuai, Peng, Lu, Cao, Xianwen, Yang, Kun, Wang, Zhen, Xiao, Yong, Xiao, Hong, Qian, Chunfa, Liu, Hongyi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8984117/
https://www.ncbi.nlm.nih.gov/pubmed/35402226
http://dx.doi.org/10.3389/fonc.2022.845009
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author Li, Yongshuai
Peng, Lu
Cao, Xianwen
Yang, Kun
Wang, Zhen
Xiao, Yong
Xiao, Hong
Qian, Chunfa
Liu, Hongyi
author_facet Li, Yongshuai
Peng, Lu
Cao, Xianwen
Yang, Kun
Wang, Zhen
Xiao, Yong
Xiao, Hong
Qian, Chunfa
Liu, Hongyi
author_sort Li, Yongshuai
collection PubMed
description HOXC cluster antisense RNA 3 (HOXC-AS3) is a long noncoding RNA (lncRNA) that plays a crucial role in various tumors; nevertheless, its role in glioma and its mechanism have not been completely elucidated. In this research, we discovered that HOXC-AS3 was over-expression in glioma cells and tissues and was associated with prognosis. Next, we determined that HOXC-AS3 targeted miR-216 as a sponge and that the F11 receptor (F11R) was the target of miR-216 by online databases analysis, qRT–PCR, and luciferase reporter assay. In addition, the rescue experiments confirmed that HOXC-AS3 regulated the expression of F11R by competitively binding miR-216 and functioning as a competing endogenous RNA (ceRNA). The intracranial glioblastoma mouse model suggested that HOXC-AS3 could promote glioma malignant progression in vivo. In summary, our study shows that the HOXC-AS3/miR-216/F11R axis plays an important role in the malignant progression of glioma, and may provide new ideas for the treatment of glioma.
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spelling pubmed-89841172022-04-07 The Long Non-Coding RNA HOXC-AS3 Promotes Glioma Progression by Sponging miR-216 to Regulate F11R Expression Li, Yongshuai Peng, Lu Cao, Xianwen Yang, Kun Wang, Zhen Xiao, Yong Xiao, Hong Qian, Chunfa Liu, Hongyi Front Oncol Oncology HOXC cluster antisense RNA 3 (HOXC-AS3) is a long noncoding RNA (lncRNA) that plays a crucial role in various tumors; nevertheless, its role in glioma and its mechanism have not been completely elucidated. In this research, we discovered that HOXC-AS3 was over-expression in glioma cells and tissues and was associated with prognosis. Next, we determined that HOXC-AS3 targeted miR-216 as a sponge and that the F11 receptor (F11R) was the target of miR-216 by online databases analysis, qRT–PCR, and luciferase reporter assay. In addition, the rescue experiments confirmed that HOXC-AS3 regulated the expression of F11R by competitively binding miR-216 and functioning as a competing endogenous RNA (ceRNA). The intracranial glioblastoma mouse model suggested that HOXC-AS3 could promote glioma malignant progression in vivo. In summary, our study shows that the HOXC-AS3/miR-216/F11R axis plays an important role in the malignant progression of glioma, and may provide new ideas for the treatment of glioma. Frontiers Media S.A. 2022-03-23 /pmc/articles/PMC8984117/ /pubmed/35402226 http://dx.doi.org/10.3389/fonc.2022.845009 Text en Copyright © 2022 Li, Peng, Cao, Yang, Wang, Xiao, Xiao, Qian and Liu https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Li, Yongshuai
Peng, Lu
Cao, Xianwen
Yang, Kun
Wang, Zhen
Xiao, Yong
Xiao, Hong
Qian, Chunfa
Liu, Hongyi
The Long Non-Coding RNA HOXC-AS3 Promotes Glioma Progression by Sponging miR-216 to Regulate F11R Expression
title The Long Non-Coding RNA HOXC-AS3 Promotes Glioma Progression by Sponging miR-216 to Regulate F11R Expression
title_full The Long Non-Coding RNA HOXC-AS3 Promotes Glioma Progression by Sponging miR-216 to Regulate F11R Expression
title_fullStr The Long Non-Coding RNA HOXC-AS3 Promotes Glioma Progression by Sponging miR-216 to Regulate F11R Expression
title_full_unstemmed The Long Non-Coding RNA HOXC-AS3 Promotes Glioma Progression by Sponging miR-216 to Regulate F11R Expression
title_short The Long Non-Coding RNA HOXC-AS3 Promotes Glioma Progression by Sponging miR-216 to Regulate F11R Expression
title_sort long non-coding rna hoxc-as3 promotes glioma progression by sponging mir-216 to regulate f11r expression
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8984117/
https://www.ncbi.nlm.nih.gov/pubmed/35402226
http://dx.doi.org/10.3389/fonc.2022.845009
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