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Visible-light-responsive reduced graphene oxide/g-C(3)N(4)/TiO(2) composite nanocoating for photoelectric stimulation of neuronal and osteoblastic differentiation

Restoration of nerve supply in newly formed bone is critical for bone defect repair. However, nerve regeneration is often overlooked when designing bone repair biomaterials. In this study, employing graphitic carbon nitride (g-C(3)N(4)) as a visible-light-driven photocatalyst and reduced graphene ox...

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Autores principales: Yan, Ziru, Li, Kai, Shao, Dandan, Shen, Qingyi, Ding, Yi, Huang, Shansong, Xie, Youtao, Zheng, Xuebin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8985170/
https://www.ncbi.nlm.nih.gov/pubmed/35424887
http://dx.doi.org/10.1039/d2ra00282e
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author Yan, Ziru
Li, Kai
Shao, Dandan
Shen, Qingyi
Ding, Yi
Huang, Shansong
Xie, Youtao
Zheng, Xuebin
author_facet Yan, Ziru
Li, Kai
Shao, Dandan
Shen, Qingyi
Ding, Yi
Huang, Shansong
Xie, Youtao
Zheng, Xuebin
author_sort Yan, Ziru
collection PubMed
description Restoration of nerve supply in newly formed bone is critical for bone defect repair. However, nerve regeneration is often overlooked when designing bone repair biomaterials. In this study, employing graphitic carbon nitride (g-C(3)N(4)) as a visible-light-driven photocatalyst and reduced graphene oxide (rGO) as a conductive interface, an rGO/g-C(3)N(4)/TiO(2) (rGO/CN/TO) ternary nanocoating with photoelectric conversion ability was fabricated on a Ti-based orthopedic implant for photoelectric stimulation of both bone and nerve repair. Compared with g-C(3)N(4)/TiO(2) (CN/TO) and TiO(2) nanocoatings, the ternary nanocoating exhibited stronger visible-light absorption as well as higher transient photocurrent density and open circuit potential under blue LED exposure. The improved photo-electrochemical properties of the ternary nanocoating were attributed to the enhanced separation of photogenerated carriers at the heterointerface. For the tested nanocoatings, introducing blue LED light irradiation enhanced MC3T3-E1 osteoblastic differentiation and neurite outgrowth of PC12 cells. Among them, the rGO/CN/TO nanocoating exerted the greatest enhancement. In a coculture system, PC12 cells on the ternary nanocoating released a higher amount of neurotransmitter calcitonin gene-related peptide (CGRP) under light irradiation, which in turn significantly enhanced osteoblastic differentiation. The results may provide a prospective approach for targeting nerve regeneration to stimulate osteogenesis when designing bone repair biomaterials.
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spelling pubmed-89851702022-04-13 Visible-light-responsive reduced graphene oxide/g-C(3)N(4)/TiO(2) composite nanocoating for photoelectric stimulation of neuronal and osteoblastic differentiation Yan, Ziru Li, Kai Shao, Dandan Shen, Qingyi Ding, Yi Huang, Shansong Xie, Youtao Zheng, Xuebin RSC Adv Chemistry Restoration of nerve supply in newly formed bone is critical for bone defect repair. However, nerve regeneration is often overlooked when designing bone repair biomaterials. In this study, employing graphitic carbon nitride (g-C(3)N(4)) as a visible-light-driven photocatalyst and reduced graphene oxide (rGO) as a conductive interface, an rGO/g-C(3)N(4)/TiO(2) (rGO/CN/TO) ternary nanocoating with photoelectric conversion ability was fabricated on a Ti-based orthopedic implant for photoelectric stimulation of both bone and nerve repair. Compared with g-C(3)N(4)/TiO(2) (CN/TO) and TiO(2) nanocoatings, the ternary nanocoating exhibited stronger visible-light absorption as well as higher transient photocurrent density and open circuit potential under blue LED exposure. The improved photo-electrochemical properties of the ternary nanocoating were attributed to the enhanced separation of photogenerated carriers at the heterointerface. For the tested nanocoatings, introducing blue LED light irradiation enhanced MC3T3-E1 osteoblastic differentiation and neurite outgrowth of PC12 cells. Among them, the rGO/CN/TO nanocoating exerted the greatest enhancement. In a coculture system, PC12 cells on the ternary nanocoating released a higher amount of neurotransmitter calcitonin gene-related peptide (CGRP) under light irradiation, which in turn significantly enhanced osteoblastic differentiation. The results may provide a prospective approach for targeting nerve regeneration to stimulate osteogenesis when designing bone repair biomaterials. The Royal Society of Chemistry 2022-03-22 /pmc/articles/PMC8985170/ /pubmed/35424887 http://dx.doi.org/10.1039/d2ra00282e Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Yan, Ziru
Li, Kai
Shao, Dandan
Shen, Qingyi
Ding, Yi
Huang, Shansong
Xie, Youtao
Zheng, Xuebin
Visible-light-responsive reduced graphene oxide/g-C(3)N(4)/TiO(2) composite nanocoating for photoelectric stimulation of neuronal and osteoblastic differentiation
title Visible-light-responsive reduced graphene oxide/g-C(3)N(4)/TiO(2) composite nanocoating for photoelectric stimulation of neuronal and osteoblastic differentiation
title_full Visible-light-responsive reduced graphene oxide/g-C(3)N(4)/TiO(2) composite nanocoating for photoelectric stimulation of neuronal and osteoblastic differentiation
title_fullStr Visible-light-responsive reduced graphene oxide/g-C(3)N(4)/TiO(2) composite nanocoating for photoelectric stimulation of neuronal and osteoblastic differentiation
title_full_unstemmed Visible-light-responsive reduced graphene oxide/g-C(3)N(4)/TiO(2) composite nanocoating for photoelectric stimulation of neuronal and osteoblastic differentiation
title_short Visible-light-responsive reduced graphene oxide/g-C(3)N(4)/TiO(2) composite nanocoating for photoelectric stimulation of neuronal and osteoblastic differentiation
title_sort visible-light-responsive reduced graphene oxide/g-c(3)n(4)/tio(2) composite nanocoating for photoelectric stimulation of neuronal and osteoblastic differentiation
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8985170/
https://www.ncbi.nlm.nih.gov/pubmed/35424887
http://dx.doi.org/10.1039/d2ra00282e
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