First glanders cases detected in Nepal underscore the need for surveillance and border controls

BACKGROUND: Glanders is a transmissible zoonotic disease caused by Burkholderia mallei that infects equids and humans. No glanders cases in equids were reported so far in Nepal. CASE PRESENTATION: Following suspected glanders in animals with clinical signs in different regions in Nepal, serum sample...

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Autores principales: P, Koirala, M, Maharjan, S, Manandhar, KR, Pandey, T, Deshayes, G, Wang, MA, Valvano, K, Laroucau
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Case Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8985341/
https://www.ncbi.nlm.nih.gov/pubmed/35387664
http://dx.doi.org/10.1186/s12917-022-03233-4
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author P, Koirala
M, Maharjan
S, Manandhar
KR, Pandey
T, Deshayes
G, Wang
MA, Valvano
K, Laroucau
author_facet P, Koirala
M, Maharjan
S, Manandhar
KR, Pandey
T, Deshayes
G, Wang
MA, Valvano
K, Laroucau
author_sort P, Koirala
collection PubMed
description BACKGROUND: Glanders is a transmissible zoonotic disease caused by Burkholderia mallei that infects equids and humans. No glanders cases in equids were reported so far in Nepal. CASE PRESENTATION: Following suspected glanders in animals with clinical signs in different regions in Nepal, serum samples were tested by CFT, ELISA and Luminex® tests. Two horses and a mule tested positive for glanders by all tests, while two other equids only tested positive by ELISA and Luminex®. Analysis of swabs and pus samples by a PCR system targeting B. mallei confirmed the presence of the bacterium in the samples collected from the 3 equids that yielded positive results in all serological tests. Genotyping of the three PCR positive samples with a SNP-based method identified a genotype closely related to the B. mallei strains circulating in India. CONCLUSION: Confirmation of glanders cases underscores the need of implementing a surveillance program in Nepal and a strict control of the animal movement across the borders.
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spelling pubmed-89853412022-04-07 First glanders cases detected in Nepal underscore the need for surveillance and border controls P, Koirala M, Maharjan S, Manandhar KR, Pandey T, Deshayes G, Wang MA, Valvano K, Laroucau BMC Vet Res Case Report BACKGROUND: Glanders is a transmissible zoonotic disease caused by Burkholderia mallei that infects equids and humans. No glanders cases in equids were reported so far in Nepal. CASE PRESENTATION: Following suspected glanders in animals with clinical signs in different regions in Nepal, serum samples were tested by CFT, ELISA and Luminex® tests. Two horses and a mule tested positive for glanders by all tests, while two other equids only tested positive by ELISA and Luminex®. Analysis of swabs and pus samples by a PCR system targeting B. mallei confirmed the presence of the bacterium in the samples collected from the 3 equids that yielded positive results in all serological tests. Genotyping of the three PCR positive samples with a SNP-based method identified a genotype closely related to the B. mallei strains circulating in India. CONCLUSION: Confirmation of glanders cases underscores the need of implementing a surveillance program in Nepal and a strict control of the animal movement across the borders. BioMed Central 2022-04-06 /pmc/articles/PMC8985341/ /pubmed/35387664 http://dx.doi.org/10.1186/s12917-022-03233-4 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Case Report
P, Koirala
M, Maharjan
S, Manandhar
KR, Pandey
T, Deshayes
G, Wang
MA, Valvano
K, Laroucau
First glanders cases detected in Nepal underscore the need for surveillance and border controls
title First glanders cases detected in Nepal underscore the need for surveillance and border controls
title_full First glanders cases detected in Nepal underscore the need for surveillance and border controls
title_fullStr First glanders cases detected in Nepal underscore the need for surveillance and border controls
title_full_unstemmed First glanders cases detected in Nepal underscore the need for surveillance and border controls
title_short First glanders cases detected in Nepal underscore the need for surveillance and border controls
title_sort first glanders cases detected in nepal underscore the need for surveillance and border controls
topic Case Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8985341/
https://www.ncbi.nlm.nih.gov/pubmed/35387664
http://dx.doi.org/10.1186/s12917-022-03233-4
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