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Evaluation of long-term stability of SARS-CoV-2 nucleic acid extracted from human nasopharyngeal samples

The maintenance of SARS-Cov-2 RNA samples poses a new challenge for laboratories and researchers. In addition, it is a requirement in order to identify what strain of the new coronavirus is predominant in a region, for instance. Therefore, it is a must to keep the quality and viability of stored RNA...

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Autores principales: de Oliveira, Priscilla Stela Santana, de Oliveira Silva, Bárbara, e Silva, Rômulo Pessoa, Galdino, Lília Vieira, de Carvalho, Vanessa Mylenna Florêncio, de Almeida, Anderson Rodrigues, da Rosa, Michelle Melgarejo, de Melo Rêgo, Moacyr Jesus Barreto, da Rocha Pitta, Maira Galdino, Pereira, Michelly Cristiny
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8985402/
https://www.ncbi.nlm.nih.gov/pubmed/35398116
http://dx.doi.org/10.1016/j.jviromet.2022.114529
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author de Oliveira, Priscilla Stela Santana
de Oliveira Silva, Bárbara
e Silva, Rômulo Pessoa
Galdino, Lília Vieira
de Carvalho, Vanessa Mylenna Florêncio
de Almeida, Anderson Rodrigues
da Rosa, Michelle Melgarejo
de Melo Rêgo, Moacyr Jesus Barreto
da Rocha Pitta, Maira Galdino
Pereira, Michelly Cristiny
author_facet de Oliveira, Priscilla Stela Santana
de Oliveira Silva, Bárbara
e Silva, Rômulo Pessoa
Galdino, Lília Vieira
de Carvalho, Vanessa Mylenna Florêncio
de Almeida, Anderson Rodrigues
da Rosa, Michelle Melgarejo
de Melo Rêgo, Moacyr Jesus Barreto
da Rocha Pitta, Maira Galdino
Pereira, Michelly Cristiny
author_sort de Oliveira, Priscilla Stela Santana
collection PubMed
description The maintenance of SARS-Cov-2 RNA samples poses a new challenge for laboratories and researchers. In addition, it is a requirement in order to identify what strain of the new coronavirus is predominant in a region, for instance. Therefore, it is a must to keep the quality and viability of stored RNA to respond to this and other valid questions. In other to test the quality of our samples and storing protocols, we randomly checked RNA samples four different times over one year using a second RT-PCR assay after the first test. The virus genes, N1 and N2, showed no significant increase in the media of the CT value between the first assay and subsequent times with p > 0.05. However, the human RP gene showed differences in the first three times analyzed, but within the acceptable sample cut-off, according to the test manufacturer. After one year, the RNA extracted from human nasopharyngeal specimens are viable to detect the virus SARS-CoV-2 genes with minor changes.
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spelling pubmed-89854022022-04-06 Evaluation of long-term stability of SARS-CoV-2 nucleic acid extracted from human nasopharyngeal samples de Oliveira, Priscilla Stela Santana de Oliveira Silva, Bárbara e Silva, Rômulo Pessoa Galdino, Lília Vieira de Carvalho, Vanessa Mylenna Florêncio de Almeida, Anderson Rodrigues da Rosa, Michelle Melgarejo de Melo Rêgo, Moacyr Jesus Barreto da Rocha Pitta, Maira Galdino Pereira, Michelly Cristiny J Virol Methods Short Communication The maintenance of SARS-Cov-2 RNA samples poses a new challenge for laboratories and researchers. In addition, it is a requirement in order to identify what strain of the new coronavirus is predominant in a region, for instance. Therefore, it is a must to keep the quality and viability of stored RNA to respond to this and other valid questions. In other to test the quality of our samples and storing protocols, we randomly checked RNA samples four different times over one year using a second RT-PCR assay after the first test. The virus genes, N1 and N2, showed no significant increase in the media of the CT value between the first assay and subsequent times with p > 0.05. However, the human RP gene showed differences in the first three times analyzed, but within the acceptable sample cut-off, according to the test manufacturer. After one year, the RNA extracted from human nasopharyngeal specimens are viable to detect the virus SARS-CoV-2 genes with minor changes. Elsevier B.V. 2022-07 2022-04-06 /pmc/articles/PMC8985402/ /pubmed/35398116 http://dx.doi.org/10.1016/j.jviromet.2022.114529 Text en © 2022 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Short Communication
de Oliveira, Priscilla Stela Santana
de Oliveira Silva, Bárbara
e Silva, Rômulo Pessoa
Galdino, Lília Vieira
de Carvalho, Vanessa Mylenna Florêncio
de Almeida, Anderson Rodrigues
da Rosa, Michelle Melgarejo
de Melo Rêgo, Moacyr Jesus Barreto
da Rocha Pitta, Maira Galdino
Pereira, Michelly Cristiny
Evaluation of long-term stability of SARS-CoV-2 nucleic acid extracted from human nasopharyngeal samples
title Evaluation of long-term stability of SARS-CoV-2 nucleic acid extracted from human nasopharyngeal samples
title_full Evaluation of long-term stability of SARS-CoV-2 nucleic acid extracted from human nasopharyngeal samples
title_fullStr Evaluation of long-term stability of SARS-CoV-2 nucleic acid extracted from human nasopharyngeal samples
title_full_unstemmed Evaluation of long-term stability of SARS-CoV-2 nucleic acid extracted from human nasopharyngeal samples
title_short Evaluation of long-term stability of SARS-CoV-2 nucleic acid extracted from human nasopharyngeal samples
title_sort evaluation of long-term stability of sars-cov-2 nucleic acid extracted from human nasopharyngeal samples
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8985402/
https://www.ncbi.nlm.nih.gov/pubmed/35398116
http://dx.doi.org/10.1016/j.jviromet.2022.114529
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