Characterization of a class II ketol-acid reductoisomerase from Mycobacterium tuberculosis

Mycobacterium tuberculosis ketol-acid reductoisomerases have been widely studied due to their metabolic importance towards development of drug-resistant bacteria treatment. We here report the biochemical characterization of a new KARI (MtKARI-II) from a Mycobacterium tuberculosis variant with a simi...

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Detalles Bibliográficos
Autores principales: Valera, Ane, Wang, Shan, Carr, Reuben, Trembleau, Laurent, Deng, Hai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2022
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8985424/
https://www.ncbi.nlm.nih.gov/pubmed/35425013
http://dx.doi.org/10.1039/d1ra08876a
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author Valera, Ane
Wang, Shan
Carr, Reuben
Trembleau, Laurent
Deng, Hai
author_facet Valera, Ane
Wang, Shan
Carr, Reuben
Trembleau, Laurent
Deng, Hai
author_sort Valera, Ane
collection PubMed
description Mycobacterium tuberculosis ketol-acid reductoisomerases have been widely studied due to their metabolic importance towards development of drug-resistant bacteria treatment. We here report the biochemical characterization of a new KARI (MtKARI-II) from a Mycobacterium tuberculosis variant with a similar kinetic profile to class I KARIs. Phylogenetic analysis suggested that MtKARI-II is clustered into a class II KARI superfamily.
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spelling pubmed-89854242022-04-13 Characterization of a class II ketol-acid reductoisomerase from Mycobacterium tuberculosis Valera, Ane Wang, Shan Carr, Reuben Trembleau, Laurent Deng, Hai RSC Adv Chemistry Mycobacterium tuberculosis ketol-acid reductoisomerases have been widely studied due to their metabolic importance towards development of drug-resistant bacteria treatment. We here report the biochemical characterization of a new KARI (MtKARI-II) from a Mycobacterium tuberculosis variant with a similar kinetic profile to class I KARIs. Phylogenetic analysis suggested that MtKARI-II is clustered into a class II KARI superfamily. The Royal Society of Chemistry 2022-04-06 /pmc/articles/PMC8985424/ /pubmed/35425013 http://dx.doi.org/10.1039/d1ra08876a Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/
spellingShingle Chemistry
Valera, Ane
Wang, Shan
Carr, Reuben
Trembleau, Laurent
Deng, Hai
Characterization of a class II ketol-acid reductoisomerase from Mycobacterium tuberculosis
title Characterization of a class II ketol-acid reductoisomerase from Mycobacterium tuberculosis
title_full Characterization of a class II ketol-acid reductoisomerase from Mycobacterium tuberculosis
title_fullStr Characterization of a class II ketol-acid reductoisomerase from Mycobacterium tuberculosis
title_full_unstemmed Characterization of a class II ketol-acid reductoisomerase from Mycobacterium tuberculosis
title_short Characterization of a class II ketol-acid reductoisomerase from Mycobacterium tuberculosis
title_sort characterization of a class ii ketol-acid reductoisomerase from mycobacterium tuberculosis
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8985424/
https://www.ncbi.nlm.nih.gov/pubmed/35425013
http://dx.doi.org/10.1039/d1ra08876a
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