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A Novel Method of CD31-Combined ABO Carbohydrate Antigen Microarray Predicts Acute Antibody-Mediated Rejection in ABO-Incompatible Kidney Transplantation

Isohemagglutinin assays employing red blood cells (RBCs) are the most common assays used to measure antibody titer in ABO-incompatible kidney transplantation (ABOi KTx). However, ABO antigens expressed on RBCs are not identical to those of kidney and antibody titers do not always correlate with clin...

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Autores principales: Tasaki, Masayuki, Tateno, Hiroaki, Sato, Takashi, Tomioka, Azusa, Kaji, Hiroyuki, Narimatsu, Hisashi, Saito, Kazuhide, Nakagawa, Yuki, Aoki, Toshinari, Kamimura, Masami, Ushiki, Takashi, Okada, Manabu, Miwa, Yuko, Hotta, Kiyohiko, Yoshida, Yutaka, Takahashi, Kota, Tomita, Yoshihiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8985549/
https://www.ncbi.nlm.nih.gov/pubmed/35401036
http://dx.doi.org/10.3389/ti.2022.10248
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author Tasaki, Masayuki
Tateno, Hiroaki
Sato, Takashi
Tomioka, Azusa
Kaji, Hiroyuki
Narimatsu, Hisashi
Saito, Kazuhide
Nakagawa, Yuki
Aoki, Toshinari
Kamimura, Masami
Ushiki, Takashi
Okada, Manabu
Miwa, Yuko
Hotta, Kiyohiko
Yoshida, Yutaka
Takahashi, Kota
Tomita, Yoshihiko
author_facet Tasaki, Masayuki
Tateno, Hiroaki
Sato, Takashi
Tomioka, Azusa
Kaji, Hiroyuki
Narimatsu, Hisashi
Saito, Kazuhide
Nakagawa, Yuki
Aoki, Toshinari
Kamimura, Masami
Ushiki, Takashi
Okada, Manabu
Miwa, Yuko
Hotta, Kiyohiko
Yoshida, Yutaka
Takahashi, Kota
Tomita, Yoshihiko
author_sort Tasaki, Masayuki
collection PubMed
description Isohemagglutinin assays employing red blood cells (RBCs) are the most common assays used to measure antibody titer in ABO-incompatible kidney transplantation (ABOi KTx). However, ABO antigens expressed on RBCs are not identical to those of kidney and antibody titers do not always correlate with clinical outcome. We previously reported that CD31 was the main protein linked to ABO antigens on kidney endothelial cells (KECs), which was different from those on RBCs. We developed a new method to measure antibody titer using a microarray of recombinant CD31 (rCD31) linked to ABO antigens (CD31-ABO microarray). Mass spectrometry analysis suggested that rCD31 and native CD31 purified from human kidney had similar ABO glycan. To confirm clinical use of CD31-ABO microarray, a total of 252 plasma samples including volunteers, hemodialysis patients, and transplant recipients were examined. In transplant recipients, any initial IgG or IgM antibody intensity >30,000 against the donor blood type in the CD31-ABO microarray showed higher sensitivity, specificity, positive predictive value, and negative predictive value of AABMR, compared to isohemagglutinin assays. Use of a CD31-ABO microarray to determine antibody titer specifically against ABO antigens expressed on KECs will contribute to precisely predicting AABMR or preventing over immunosuppression following ABOi KTx.
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spelling pubmed-89855492022-04-07 A Novel Method of CD31-Combined ABO Carbohydrate Antigen Microarray Predicts Acute Antibody-Mediated Rejection in ABO-Incompatible Kidney Transplantation Tasaki, Masayuki Tateno, Hiroaki Sato, Takashi Tomioka, Azusa Kaji, Hiroyuki Narimatsu, Hisashi Saito, Kazuhide Nakagawa, Yuki Aoki, Toshinari Kamimura, Masami Ushiki, Takashi Okada, Manabu Miwa, Yuko Hotta, Kiyohiko Yoshida, Yutaka Takahashi, Kota Tomita, Yoshihiko Transpl Int Health Archive Isohemagglutinin assays employing red blood cells (RBCs) are the most common assays used to measure antibody titer in ABO-incompatible kidney transplantation (ABOi KTx). However, ABO antigens expressed on RBCs are not identical to those of kidney and antibody titers do not always correlate with clinical outcome. We previously reported that CD31 was the main protein linked to ABO antigens on kidney endothelial cells (KECs), which was different from those on RBCs. We developed a new method to measure antibody titer using a microarray of recombinant CD31 (rCD31) linked to ABO antigens (CD31-ABO microarray). Mass spectrometry analysis suggested that rCD31 and native CD31 purified from human kidney had similar ABO glycan. To confirm clinical use of CD31-ABO microarray, a total of 252 plasma samples including volunteers, hemodialysis patients, and transplant recipients were examined. In transplant recipients, any initial IgG or IgM antibody intensity >30,000 against the donor blood type in the CD31-ABO microarray showed higher sensitivity, specificity, positive predictive value, and negative predictive value of AABMR, compared to isohemagglutinin assays. Use of a CD31-ABO microarray to determine antibody titer specifically against ABO antigens expressed on KECs will contribute to precisely predicting AABMR or preventing over immunosuppression following ABOi KTx. Frontiers Media S.A. 2022-03-23 /pmc/articles/PMC8985549/ /pubmed/35401036 http://dx.doi.org/10.3389/ti.2022.10248 Text en Copyright © 2022 Tasaki, Tateno, Sato, Tomioka, Kaji, Narimatsu, Saito, Nakagawa, Aoki, Kamimura, Ushiki, Okada, Miwa, Hotta, Yoshida, Takahashi and Tomita. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Health Archive
Tasaki, Masayuki
Tateno, Hiroaki
Sato, Takashi
Tomioka, Azusa
Kaji, Hiroyuki
Narimatsu, Hisashi
Saito, Kazuhide
Nakagawa, Yuki
Aoki, Toshinari
Kamimura, Masami
Ushiki, Takashi
Okada, Manabu
Miwa, Yuko
Hotta, Kiyohiko
Yoshida, Yutaka
Takahashi, Kota
Tomita, Yoshihiko
A Novel Method of CD31-Combined ABO Carbohydrate Antigen Microarray Predicts Acute Antibody-Mediated Rejection in ABO-Incompatible Kidney Transplantation
title A Novel Method of CD31-Combined ABO Carbohydrate Antigen Microarray Predicts Acute Antibody-Mediated Rejection in ABO-Incompatible Kidney Transplantation
title_full A Novel Method of CD31-Combined ABO Carbohydrate Antigen Microarray Predicts Acute Antibody-Mediated Rejection in ABO-Incompatible Kidney Transplantation
title_fullStr A Novel Method of CD31-Combined ABO Carbohydrate Antigen Microarray Predicts Acute Antibody-Mediated Rejection in ABO-Incompatible Kidney Transplantation
title_full_unstemmed A Novel Method of CD31-Combined ABO Carbohydrate Antigen Microarray Predicts Acute Antibody-Mediated Rejection in ABO-Incompatible Kidney Transplantation
title_short A Novel Method of CD31-Combined ABO Carbohydrate Antigen Microarray Predicts Acute Antibody-Mediated Rejection in ABO-Incompatible Kidney Transplantation
title_sort novel method of cd31-combined abo carbohydrate antigen microarray predicts acute antibody-mediated rejection in abo-incompatible kidney transplantation
topic Health Archive
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8985549/
https://www.ncbi.nlm.nih.gov/pubmed/35401036
http://dx.doi.org/10.3389/ti.2022.10248
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