Evaluation of the Antidiabetic Potential of an Isolated Hydroalcoholic Fraction from the Fruit of Withania coagulans

The hydro-alcoholic extract of Withania coagulans fruits was investigated for preliminary phytochemical screening and characterized by high-performance thin-layer chromatography. Column chromatography of the hydro-alcoholic extract of W. coagulans eluted with four different combinations of ethyl ace...

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Autores principales: Waris, Md., Shahzad, Naiyer, Al-Ghamdi, Saeed Saeed, Mir, Showkat Rasool, Tanuja
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer - Medknow 2021
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8985838/
https://www.ncbi.nlm.nih.gov/pubmed/35399799
http://dx.doi.org/10.4103/jpbs.JPBS_423_20
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author Waris, Md.
Shahzad, Naiyer
Al-Ghamdi, Saeed Saeed
Mir, Showkat Rasool
Tanuja,
author_facet Waris, Md.
Shahzad, Naiyer
Al-Ghamdi, Saeed Saeed
Mir, Showkat Rasool
Tanuja,
author_sort Waris, Md.
collection PubMed
description The hydro-alcoholic extract of Withania coagulans fruits was investigated for preliminary phytochemical screening and characterized by high-performance thin-layer chromatography. Column chromatography of the hydro-alcoholic extract of W. coagulans eluted with four different combinations of ethyl acetate and methanol yielded four fractions (WCF(01), WCF(02), WCF(03,) and WCF(04)). One of these fractions, WCF(02), significantly (P < 0.05) inhibited in vitro α-amylase and α-glucosidase activity with IC(50) values of 104.71 μg/mL and 70.79 μg/mL, respectively. WCF(02) further reduced blood glucose levels in comparison to control in the starch tolerance test. The extract showed a relative dose-dependent effect. It was observed that none of the extracts could delay the peak blood glucose that was achieved after 60 min of carbohydrate challenge, but these blunted the glycemic peak.
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spelling pubmed-89858382022-04-07 Evaluation of the Antidiabetic Potential of an Isolated Hydroalcoholic Fraction from the Fruit of Withania coagulans Waris, Md. Shahzad, Naiyer Al-Ghamdi, Saeed Saeed Mir, Showkat Rasool Tanuja, J Pharm Bioallied Sci Original Article The hydro-alcoholic extract of Withania coagulans fruits was investigated for preliminary phytochemical screening and characterized by high-performance thin-layer chromatography. Column chromatography of the hydro-alcoholic extract of W. coagulans eluted with four different combinations of ethyl acetate and methanol yielded four fractions (WCF(01), WCF(02), WCF(03,) and WCF(04)). One of these fractions, WCF(02), significantly (P < 0.05) inhibited in vitro α-amylase and α-glucosidase activity with IC(50) values of 104.71 μg/mL and 70.79 μg/mL, respectively. WCF(02) further reduced blood glucose levels in comparison to control in the starch tolerance test. The extract showed a relative dose-dependent effect. It was observed that none of the extracts could delay the peak blood glucose that was achieved after 60 min of carbohydrate challenge, but these blunted the glycemic peak. Wolters Kluwer - Medknow 2021 2022-03-04 /pmc/articles/PMC8985838/ /pubmed/35399799 http://dx.doi.org/10.4103/jpbs.JPBS_423_20 Text en Copyright: © 2022 Journal of Pharmacy And Bioallied Sciences https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Waris, Md.
Shahzad, Naiyer
Al-Ghamdi, Saeed Saeed
Mir, Showkat Rasool
Tanuja,
Evaluation of the Antidiabetic Potential of an Isolated Hydroalcoholic Fraction from the Fruit of Withania coagulans
title Evaluation of the Antidiabetic Potential of an Isolated Hydroalcoholic Fraction from the Fruit of Withania coagulans
title_full Evaluation of the Antidiabetic Potential of an Isolated Hydroalcoholic Fraction from the Fruit of Withania coagulans
title_fullStr Evaluation of the Antidiabetic Potential of an Isolated Hydroalcoholic Fraction from the Fruit of Withania coagulans
title_full_unstemmed Evaluation of the Antidiabetic Potential of an Isolated Hydroalcoholic Fraction from the Fruit of Withania coagulans
title_short Evaluation of the Antidiabetic Potential of an Isolated Hydroalcoholic Fraction from the Fruit of Withania coagulans
title_sort evaluation of the antidiabetic potential of an isolated hydroalcoholic fraction from the fruit of withania coagulans
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8985838/
https://www.ncbi.nlm.nih.gov/pubmed/35399799
http://dx.doi.org/10.4103/jpbs.JPBS_423_20
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