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Multidrug-resistant and enterotoxigenic methicillin-resistant Staphylococcus aureus isolated from raw milk of cows at small-scale production units
OBJECTIVE: Staphylococcus aureus (S. aureus) has evolved as one of the most significant bacteria causing food poisoning outbreaks worldwide. This study was carried out to investigate the prevalence, antibiotic sensitivity, virulence, and enterotoxin production of S. aureus in raw milk of cow from sm...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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A periodical of the Network for the Veterinarians of Bangladesh (BDvetNET)
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8985885/ https://www.ncbi.nlm.nih.gov/pubmed/35445112 http://dx.doi.org/10.5455/javar.2022.i575 |
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author | Eid, Hamza Mohamed El-Mahallawy, Heba Sayed Mohammed, Sahar Roshdi Mohammed, Nehal Ezzat Yousef Eidaroos, Nada Hussein |
author_facet | Eid, Hamza Mohamed El-Mahallawy, Heba Sayed Mohammed, Sahar Roshdi Mohammed, Nehal Ezzat Yousef Eidaroos, Nada Hussein |
author_sort | Eid, Hamza Mohamed |
collection | PubMed |
description | OBJECTIVE: Staphylococcus aureus (S. aureus) has evolved as one of the most significant bacteria causing food poisoning outbreaks worldwide. This study was carried out to investigate the prevalence, antibiotic sensitivity, virulence, and enterotoxin production of S. aureus in raw milk of cow from small-scale production units and house-raised animals in Damietta governorate, Egypt. MATERIAL AND METHODS: The samples were examined bacteriologically, and antimicrobial sensitivity testing was carried out. Moreover, isolates were characterized by the molecular detection of antimicrobial resistance, virulence, and enterotoxin genes. RESULTS: Out of 300 milk samples examined, S. aureus was isolated from 50 samples (16.7%). Antibiotic sensitivity testing revealed that isolates were resistant to β-lactams (32%), tetracycline (16%), and norfloxacin (16%); however, they showed considerable sensitivity to ceftaroline and amikacin (72%). Multidrug-resistance (MDR) has been observed in eight isolates (16%), with a MDR index (0.5) in all of them. Of the total S. aureus isolates obtained, methicillin-resistant S. aureus (MRSA) has been confirmed molecularly in 16/50 (32%) and was found to carry mecA and coa genes, while virulence genes; hlg (11/16, 68.75%) and tsst (6/16, 37.5%) were amplified at a lower percentage, and they showed a significant moderate negative correlation (r = −0.59, p-value > 0.05). Antibiotic resistance genes have been detected in resistant isolates relevant to their phenotypic resistance: blaZ (100%), tetK (50%), and norA (50%). Fifty percent of MRSA isolates carried the seb enterotoxin gene. CONCLUSION: High detection rate of MRSA and MDR isolates from milk necessitates the prompt implementation of efficient antimicrobial stewardship guidelines, especially at neglected small-scale production units. |
format | Online Article Text |
id | pubmed-8985885 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | A periodical of the Network for the Veterinarians of Bangladesh (BDvetNET) |
record_format | MEDLINE/PubMed |
spelling | pubmed-89858852022-04-19 Multidrug-resistant and enterotoxigenic methicillin-resistant Staphylococcus aureus isolated from raw milk of cows at small-scale production units Eid, Hamza Mohamed El-Mahallawy, Heba Sayed Mohammed, Sahar Roshdi Mohammed, Nehal Ezzat Yousef Eidaroos, Nada Hussein J Adv Vet Anim Res Original Article OBJECTIVE: Staphylococcus aureus (S. aureus) has evolved as one of the most significant bacteria causing food poisoning outbreaks worldwide. This study was carried out to investigate the prevalence, antibiotic sensitivity, virulence, and enterotoxin production of S. aureus in raw milk of cow from small-scale production units and house-raised animals in Damietta governorate, Egypt. MATERIAL AND METHODS: The samples were examined bacteriologically, and antimicrobial sensitivity testing was carried out. Moreover, isolates were characterized by the molecular detection of antimicrobial resistance, virulence, and enterotoxin genes. RESULTS: Out of 300 milk samples examined, S. aureus was isolated from 50 samples (16.7%). Antibiotic sensitivity testing revealed that isolates were resistant to β-lactams (32%), tetracycline (16%), and norfloxacin (16%); however, they showed considerable sensitivity to ceftaroline and amikacin (72%). Multidrug-resistance (MDR) has been observed in eight isolates (16%), with a MDR index (0.5) in all of them. Of the total S. aureus isolates obtained, methicillin-resistant S. aureus (MRSA) has been confirmed molecularly in 16/50 (32%) and was found to carry mecA and coa genes, while virulence genes; hlg (11/16, 68.75%) and tsst (6/16, 37.5%) were amplified at a lower percentage, and they showed a significant moderate negative correlation (r = −0.59, p-value > 0.05). Antibiotic resistance genes have been detected in resistant isolates relevant to their phenotypic resistance: blaZ (100%), tetK (50%), and norA (50%). Fifty percent of MRSA isolates carried the seb enterotoxin gene. CONCLUSION: High detection rate of MRSA and MDR isolates from milk necessitates the prompt implementation of efficient antimicrobial stewardship guidelines, especially at neglected small-scale production units. A periodical of the Network for the Veterinarians of Bangladesh (BDvetNET) 2022-03-10 /pmc/articles/PMC8985885/ /pubmed/35445112 http://dx.doi.org/10.5455/javar.2022.i575 Text en Copyright: © Journal of Advanced Veterinary and Animal Research https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 License (http://creativecommons.org/licenses/by/4.0 (https://creativecommons.org/licenses/by/4.0/) ) |
spellingShingle | Original Article Eid, Hamza Mohamed El-Mahallawy, Heba Sayed Mohammed, Sahar Roshdi Mohammed, Nehal Ezzat Yousef Eidaroos, Nada Hussein Multidrug-resistant and enterotoxigenic methicillin-resistant Staphylococcus aureus isolated from raw milk of cows at small-scale production units |
title | Multidrug-resistant and enterotoxigenic methicillin-resistant Staphylococcus aureus isolated from raw milk of cows at small-scale production units |
title_full | Multidrug-resistant and enterotoxigenic methicillin-resistant Staphylococcus aureus isolated from raw milk of cows at small-scale production units |
title_fullStr | Multidrug-resistant and enterotoxigenic methicillin-resistant Staphylococcus aureus isolated from raw milk of cows at small-scale production units |
title_full_unstemmed | Multidrug-resistant and enterotoxigenic methicillin-resistant Staphylococcus aureus isolated from raw milk of cows at small-scale production units |
title_short | Multidrug-resistant and enterotoxigenic methicillin-resistant Staphylococcus aureus isolated from raw milk of cows at small-scale production units |
title_sort | multidrug-resistant and enterotoxigenic methicillin-resistant staphylococcus aureus isolated from raw milk of cows at small-scale production units |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8985885/ https://www.ncbi.nlm.nih.gov/pubmed/35445112 http://dx.doi.org/10.5455/javar.2022.i575 |
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