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Multidrug-resistant and enterotoxigenic methicillin-resistant Staphylococcus aureus isolated from raw milk of cows at small-scale production units

OBJECTIVE: Staphylococcus aureus (S. aureus) has evolved as one of the most significant bacteria causing food poisoning outbreaks worldwide. This study was carried out to investigate the prevalence, antibiotic sensitivity, virulence, and enterotoxin production of S. aureus in raw milk of cow from sm...

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Autores principales: Eid, Hamza Mohamed, El-Mahallawy, Heba Sayed, Mohammed, Sahar Roshdi, Mohammed, Nehal Ezzat Yousef, Eidaroos, Nada Hussein
Formato: Online Artículo Texto
Lenguaje:English
Publicado: A periodical of the Network for the Veterinarians of Bangladesh (BDvetNET) 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8985885/
https://www.ncbi.nlm.nih.gov/pubmed/35445112
http://dx.doi.org/10.5455/javar.2022.i575
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author Eid, Hamza Mohamed
El-Mahallawy, Heba Sayed
Mohammed, Sahar Roshdi
Mohammed, Nehal Ezzat Yousef
Eidaroos, Nada Hussein
author_facet Eid, Hamza Mohamed
El-Mahallawy, Heba Sayed
Mohammed, Sahar Roshdi
Mohammed, Nehal Ezzat Yousef
Eidaroos, Nada Hussein
author_sort Eid, Hamza Mohamed
collection PubMed
description OBJECTIVE: Staphylococcus aureus (S. aureus) has evolved as one of the most significant bacteria causing food poisoning outbreaks worldwide. This study was carried out to investigate the prevalence, antibiotic sensitivity, virulence, and enterotoxin production of S. aureus in raw milk of cow from small-scale production units and house-raised animals in Damietta governorate, Egypt. MATERIAL AND METHODS: The samples were examined bacteriologically, and antimicrobial sensitivity testing was carried out. Moreover, isolates were characterized by the molecular detection of antimicrobial resistance, virulence, and enterotoxin genes. RESULTS: Out of 300 milk samples examined, S. aureus was isolated from 50 samples (16.7%). Antibiotic sensitivity testing revealed that isolates were resistant to β-lactams (32%), tetracycline (16%), and norfloxacin (16%); however, they showed considerable sensitivity to ceftaroline and amikacin (72%). Multidrug-resistance (MDR) has been observed in eight isolates (16%), with a MDR index (0.5) in all of them. Of the total S. aureus isolates obtained, methicillin-resistant S. aureus (MRSA) has been confirmed molecularly in 16/50 (32%) and was found to carry mecA and coa genes, while virulence genes; hlg (11/16, 68.75%) and tsst (6/16, 37.5%) were amplified at a lower percentage, and they showed a significant moderate negative correlation (r = −0.59, p-value > 0.05). Antibiotic resistance genes have been detected in resistant isolates relevant to their phenotypic resistance: blaZ (100%), tetK (50%), and norA (50%). Fifty percent of MRSA isolates carried the seb enterotoxin gene. CONCLUSION: High detection rate of MRSA and MDR isolates from milk necessitates the prompt implementation of efficient antimicrobial stewardship guidelines, especially at neglected small-scale production units.
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spelling pubmed-89858852022-04-19 Multidrug-resistant and enterotoxigenic methicillin-resistant Staphylococcus aureus isolated from raw milk of cows at small-scale production units Eid, Hamza Mohamed El-Mahallawy, Heba Sayed Mohammed, Sahar Roshdi Mohammed, Nehal Ezzat Yousef Eidaroos, Nada Hussein J Adv Vet Anim Res Original Article OBJECTIVE: Staphylococcus aureus (S. aureus) has evolved as one of the most significant bacteria causing food poisoning outbreaks worldwide. This study was carried out to investigate the prevalence, antibiotic sensitivity, virulence, and enterotoxin production of S. aureus in raw milk of cow from small-scale production units and house-raised animals in Damietta governorate, Egypt. MATERIAL AND METHODS: The samples were examined bacteriologically, and antimicrobial sensitivity testing was carried out. Moreover, isolates were characterized by the molecular detection of antimicrobial resistance, virulence, and enterotoxin genes. RESULTS: Out of 300 milk samples examined, S. aureus was isolated from 50 samples (16.7%). Antibiotic sensitivity testing revealed that isolates were resistant to β-lactams (32%), tetracycline (16%), and norfloxacin (16%); however, they showed considerable sensitivity to ceftaroline and amikacin (72%). Multidrug-resistance (MDR) has been observed in eight isolates (16%), with a MDR index (0.5) in all of them. Of the total S. aureus isolates obtained, methicillin-resistant S. aureus (MRSA) has been confirmed molecularly in 16/50 (32%) and was found to carry mecA and coa genes, while virulence genes; hlg (11/16, 68.75%) and tsst (6/16, 37.5%) were amplified at a lower percentage, and they showed a significant moderate negative correlation (r = −0.59, p-value > 0.05). Antibiotic resistance genes have been detected in resistant isolates relevant to their phenotypic resistance: blaZ (100%), tetK (50%), and norA (50%). Fifty percent of MRSA isolates carried the seb enterotoxin gene. CONCLUSION: High detection rate of MRSA and MDR isolates from milk necessitates the prompt implementation of efficient antimicrobial stewardship guidelines, especially at neglected small-scale production units. A periodical of the Network for the Veterinarians of Bangladesh (BDvetNET) 2022-03-10 /pmc/articles/PMC8985885/ /pubmed/35445112 http://dx.doi.org/10.5455/javar.2022.i575 Text en Copyright: © Journal of Advanced Veterinary and Animal Research https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 License (http://creativecommons.org/licenses/by/4.0 (https://creativecommons.org/licenses/by/4.0/) )
spellingShingle Original Article
Eid, Hamza Mohamed
El-Mahallawy, Heba Sayed
Mohammed, Sahar Roshdi
Mohammed, Nehal Ezzat Yousef
Eidaroos, Nada Hussein
Multidrug-resistant and enterotoxigenic methicillin-resistant Staphylococcus aureus isolated from raw milk of cows at small-scale production units
title Multidrug-resistant and enterotoxigenic methicillin-resistant Staphylococcus aureus isolated from raw milk of cows at small-scale production units
title_full Multidrug-resistant and enterotoxigenic methicillin-resistant Staphylococcus aureus isolated from raw milk of cows at small-scale production units
title_fullStr Multidrug-resistant and enterotoxigenic methicillin-resistant Staphylococcus aureus isolated from raw milk of cows at small-scale production units
title_full_unstemmed Multidrug-resistant and enterotoxigenic methicillin-resistant Staphylococcus aureus isolated from raw milk of cows at small-scale production units
title_short Multidrug-resistant and enterotoxigenic methicillin-resistant Staphylococcus aureus isolated from raw milk of cows at small-scale production units
title_sort multidrug-resistant and enterotoxigenic methicillin-resistant staphylococcus aureus isolated from raw milk of cows at small-scale production units
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8985885/
https://www.ncbi.nlm.nih.gov/pubmed/35445112
http://dx.doi.org/10.5455/javar.2022.i575
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