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Spontaneous evolution of equilibrium morphology in phospholipid-cholesterol monolayers

Competition between intradomain electrostatic repulsions and interdomain line tension leads to domain shape transitions in phase-separating lipid monolayers. The question remains if these morphologies are energy minima or are kinetically trapped metastable states. We show the reversible evolution of...

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Autores principales: Valtierrez-Gaytan, Cain, Barakat, Joseph M., Kohler, Mitchell, Kieu, Khanh, Stottrup, Benjamin L., Zasadzinski, Joseph A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8986108/
https://www.ncbi.nlm.nih.gov/pubmed/35385307
http://dx.doi.org/10.1126/sciadv.abl9152
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author Valtierrez-Gaytan, Cain
Barakat, Joseph M.
Kohler, Mitchell
Kieu, Khanh
Stottrup, Benjamin L.
Zasadzinski, Joseph A.
author_facet Valtierrez-Gaytan, Cain
Barakat, Joseph M.
Kohler, Mitchell
Kieu, Khanh
Stottrup, Benjamin L.
Zasadzinski, Joseph A.
author_sort Valtierrez-Gaytan, Cain
collection PubMed
description Competition between intradomain electrostatic repulsions and interdomain line tension leads to domain shape transitions in phase-separating lipid monolayers. The question remains if these morphologies are energy minima or are kinetically trapped metastable states. We show the reversible evolution of uniform width stripe domains from polydisperse semicircular domains in monolayers of dipalmitoylphosphatidylcholine (DPPC), hexadecanol (HD) or palmitic acid (PA), and dihydrocholesterol (DChol). The initial semicircular domains grow at a fixed 2:1 DPPC:HD (or PA) stoichiometry, depleting the liquid phase of HD, leaving behind a liquid enriched in DPPC and DChol. At higher surface pressures, the remaining DPPC precipitates onto existing domains, decreasing the ratio of line tension to the square of the dipole density difference, λ/μ(2). Theory predicts that, as λ/μ(2) decreases, circular domains reversibly transform to uniform width stripes as the minimum energy structure. Measuring the stripe width provides the first estimates of λ/μ(2) at liquid condensed–liquid expanded phase coexistence.
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spelling pubmed-89861082022-04-19 Spontaneous evolution of equilibrium morphology in phospholipid-cholesterol monolayers Valtierrez-Gaytan, Cain Barakat, Joseph M. Kohler, Mitchell Kieu, Khanh Stottrup, Benjamin L. Zasadzinski, Joseph A. Sci Adv Physical and Materials Sciences Competition between intradomain electrostatic repulsions and interdomain line tension leads to domain shape transitions in phase-separating lipid monolayers. The question remains if these morphologies are energy minima or are kinetically trapped metastable states. We show the reversible evolution of uniform width stripe domains from polydisperse semicircular domains in monolayers of dipalmitoylphosphatidylcholine (DPPC), hexadecanol (HD) or palmitic acid (PA), and dihydrocholesterol (DChol). The initial semicircular domains grow at a fixed 2:1 DPPC:HD (or PA) stoichiometry, depleting the liquid phase of HD, leaving behind a liquid enriched in DPPC and DChol. At higher surface pressures, the remaining DPPC precipitates onto existing domains, decreasing the ratio of line tension to the square of the dipole density difference, λ/μ(2). Theory predicts that, as λ/μ(2) decreases, circular domains reversibly transform to uniform width stripes as the minimum energy structure. Measuring the stripe width provides the first estimates of λ/μ(2) at liquid condensed–liquid expanded phase coexistence. American Association for the Advancement of Science 2022-04-06 /pmc/articles/PMC8986108/ /pubmed/35385307 http://dx.doi.org/10.1126/sciadv.abl9152 Text en Copyright © 2022 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY). https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution license (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Physical and Materials Sciences
Valtierrez-Gaytan, Cain
Barakat, Joseph M.
Kohler, Mitchell
Kieu, Khanh
Stottrup, Benjamin L.
Zasadzinski, Joseph A.
Spontaneous evolution of equilibrium morphology in phospholipid-cholesterol monolayers
title Spontaneous evolution of equilibrium morphology in phospholipid-cholesterol monolayers
title_full Spontaneous evolution of equilibrium morphology in phospholipid-cholesterol monolayers
title_fullStr Spontaneous evolution of equilibrium morphology in phospholipid-cholesterol monolayers
title_full_unstemmed Spontaneous evolution of equilibrium morphology in phospholipid-cholesterol monolayers
title_short Spontaneous evolution of equilibrium morphology in phospholipid-cholesterol monolayers
title_sort spontaneous evolution of equilibrium morphology in phospholipid-cholesterol monolayers
topic Physical and Materials Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8986108/
https://www.ncbi.nlm.nih.gov/pubmed/35385307
http://dx.doi.org/10.1126/sciadv.abl9152
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