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Inhibiting host-protein deposition on urinary catheters reduces associated urinary tract infections
Microbial adhesion to medical devices is common for hospital-acquired infections, particularly for urinary catheters. If not properly treated these infections cause complications and exacerbate antimicrobial resistance. Catheter use elicits bladder inflammation, releasing host serum proteins, includ...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8986317/ https://www.ncbi.nlm.nih.gov/pubmed/35348114 http://dx.doi.org/10.7554/eLife.75798 |
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author | Andersen, Marissa Jeme Fong, ChunKi La Bella, Alyssa Ann Molina, Jonathan Jesus Molesan, Alex Champion, Matthew M Howell, Caitlin Flores-Mireles, Ana L |
author_facet | Andersen, Marissa Jeme Fong, ChunKi La Bella, Alyssa Ann Molina, Jonathan Jesus Molesan, Alex Champion, Matthew M Howell, Caitlin Flores-Mireles, Ana L |
author_sort | Andersen, Marissa Jeme |
collection | PubMed |
description | Microbial adhesion to medical devices is common for hospital-acquired infections, particularly for urinary catheters. If not properly treated these infections cause complications and exacerbate antimicrobial resistance. Catheter use elicits bladder inflammation, releasing host serum proteins, including fibrinogen (Fg), into the bladder, which deposit on the urinary catheter. Enterococcus faecalis uses Fg as a scaffold to bind and persist in the bladder despite antibiotic treatments. Inhibition of Fg–pathogen interaction significantly reduces infection. Here, we show deposited Fg is advantageous for uropathogens E. faecalis, Escherichia coli, Pseudomonas aeruginosa, K. pneumoniae, A. baumannii, and C. albicans, suggesting that targeting catheter protein deposition may reduce colonization creating an effective intervention for catheter-associated urinary tract infections (CAUTIs). In a mouse model of CAUTI, host-protein deposition was reduced, using liquid-infused silicone catheters, resulting in decreased colonization on catheters, in bladders, and dissemination in vivo. Furthermore, proteomics revealed a significant decrease in deposition of host-secreted proteins on liquid-infused catheter surfaces. Our findings suggest targeting microbial-binding scaffolds may be an effective antibiotic-sparing intervention for use against CAUTIs and other medical device infections. |
format | Online Article Text |
id | pubmed-8986317 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-89863172022-04-07 Inhibiting host-protein deposition on urinary catheters reduces associated urinary tract infections Andersen, Marissa Jeme Fong, ChunKi La Bella, Alyssa Ann Molina, Jonathan Jesus Molesan, Alex Champion, Matthew M Howell, Caitlin Flores-Mireles, Ana L eLife Microbiology and Infectious Disease Microbial adhesion to medical devices is common for hospital-acquired infections, particularly for urinary catheters. If not properly treated these infections cause complications and exacerbate antimicrobial resistance. Catheter use elicits bladder inflammation, releasing host serum proteins, including fibrinogen (Fg), into the bladder, which deposit on the urinary catheter. Enterococcus faecalis uses Fg as a scaffold to bind and persist in the bladder despite antibiotic treatments. Inhibition of Fg–pathogen interaction significantly reduces infection. Here, we show deposited Fg is advantageous for uropathogens E. faecalis, Escherichia coli, Pseudomonas aeruginosa, K. pneumoniae, A. baumannii, and C. albicans, suggesting that targeting catheter protein deposition may reduce colonization creating an effective intervention for catheter-associated urinary tract infections (CAUTIs). In a mouse model of CAUTI, host-protein deposition was reduced, using liquid-infused silicone catheters, resulting in decreased colonization on catheters, in bladders, and dissemination in vivo. Furthermore, proteomics revealed a significant decrease in deposition of host-secreted proteins on liquid-infused catheter surfaces. Our findings suggest targeting microbial-binding scaffolds may be an effective antibiotic-sparing intervention for use against CAUTIs and other medical device infections. eLife Sciences Publications, Ltd 2022-03-29 /pmc/articles/PMC8986317/ /pubmed/35348114 http://dx.doi.org/10.7554/eLife.75798 Text en © 2022, Andersen et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Microbiology and Infectious Disease Andersen, Marissa Jeme Fong, ChunKi La Bella, Alyssa Ann Molina, Jonathan Jesus Molesan, Alex Champion, Matthew M Howell, Caitlin Flores-Mireles, Ana L Inhibiting host-protein deposition on urinary catheters reduces associated urinary tract infections |
title | Inhibiting host-protein deposition on urinary catheters reduces associated urinary tract infections |
title_full | Inhibiting host-protein deposition on urinary catheters reduces associated urinary tract infections |
title_fullStr | Inhibiting host-protein deposition on urinary catheters reduces associated urinary tract infections |
title_full_unstemmed | Inhibiting host-protein deposition on urinary catheters reduces associated urinary tract infections |
title_short | Inhibiting host-protein deposition on urinary catheters reduces associated urinary tract infections |
title_sort | inhibiting host-protein deposition on urinary catheters reduces associated urinary tract infections |
topic | Microbiology and Infectious Disease |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8986317/ https://www.ncbi.nlm.nih.gov/pubmed/35348114 http://dx.doi.org/10.7554/eLife.75798 |
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