Cargando…

Inhibiting host-protein deposition on urinary catheters reduces associated urinary tract infections

Microbial adhesion to medical devices is common for hospital-acquired infections, particularly for urinary catheters. If not properly treated these infections cause complications and exacerbate antimicrobial resistance. Catheter use elicits bladder inflammation, releasing host serum proteins, includ...

Descripción completa

Detalles Bibliográficos
Autores principales: Andersen, Marissa Jeme, Fong, ChunKi, La Bella, Alyssa Ann, Molina, Jonathan Jesus, Molesan, Alex, Champion, Matthew M, Howell, Caitlin, Flores-Mireles, Ana L
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8986317/
https://www.ncbi.nlm.nih.gov/pubmed/35348114
http://dx.doi.org/10.7554/eLife.75798
_version_ 1784682518854762496
author Andersen, Marissa Jeme
Fong, ChunKi
La Bella, Alyssa Ann
Molina, Jonathan Jesus
Molesan, Alex
Champion, Matthew M
Howell, Caitlin
Flores-Mireles, Ana L
author_facet Andersen, Marissa Jeme
Fong, ChunKi
La Bella, Alyssa Ann
Molina, Jonathan Jesus
Molesan, Alex
Champion, Matthew M
Howell, Caitlin
Flores-Mireles, Ana L
author_sort Andersen, Marissa Jeme
collection PubMed
description Microbial adhesion to medical devices is common for hospital-acquired infections, particularly for urinary catheters. If not properly treated these infections cause complications and exacerbate antimicrobial resistance. Catheter use elicits bladder inflammation, releasing host serum proteins, including fibrinogen (Fg), into the bladder, which deposit on the urinary catheter. Enterococcus faecalis uses Fg as a scaffold to bind and persist in the bladder despite antibiotic treatments. Inhibition of Fg–pathogen interaction significantly reduces infection. Here, we show deposited Fg is advantageous for uropathogens E. faecalis, Escherichia coli, Pseudomonas aeruginosa, K. pneumoniae, A. baumannii, and C. albicans, suggesting that targeting catheter protein deposition may reduce colonization creating an effective intervention for catheter-associated urinary tract infections (CAUTIs). In a mouse model of CAUTI, host-protein deposition was reduced, using liquid-infused silicone catheters, resulting in decreased colonization on catheters, in bladders, and dissemination in vivo. Furthermore, proteomics revealed a significant decrease in deposition of host-secreted proteins on liquid-infused catheter surfaces. Our findings suggest targeting microbial-binding scaffolds may be an effective antibiotic-sparing intervention for use against CAUTIs and other medical device infections.
format Online
Article
Text
id pubmed-8986317
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher eLife Sciences Publications, Ltd
record_format MEDLINE/PubMed
spelling pubmed-89863172022-04-07 Inhibiting host-protein deposition on urinary catheters reduces associated urinary tract infections Andersen, Marissa Jeme Fong, ChunKi La Bella, Alyssa Ann Molina, Jonathan Jesus Molesan, Alex Champion, Matthew M Howell, Caitlin Flores-Mireles, Ana L eLife Microbiology and Infectious Disease Microbial adhesion to medical devices is common for hospital-acquired infections, particularly for urinary catheters. If not properly treated these infections cause complications and exacerbate antimicrobial resistance. Catheter use elicits bladder inflammation, releasing host serum proteins, including fibrinogen (Fg), into the bladder, which deposit on the urinary catheter. Enterococcus faecalis uses Fg as a scaffold to bind and persist in the bladder despite antibiotic treatments. Inhibition of Fg–pathogen interaction significantly reduces infection. Here, we show deposited Fg is advantageous for uropathogens E. faecalis, Escherichia coli, Pseudomonas aeruginosa, K. pneumoniae, A. baumannii, and C. albicans, suggesting that targeting catheter protein deposition may reduce colonization creating an effective intervention for catheter-associated urinary tract infections (CAUTIs). In a mouse model of CAUTI, host-protein deposition was reduced, using liquid-infused silicone catheters, resulting in decreased colonization on catheters, in bladders, and dissemination in vivo. Furthermore, proteomics revealed a significant decrease in deposition of host-secreted proteins on liquid-infused catheter surfaces. Our findings suggest targeting microbial-binding scaffolds may be an effective antibiotic-sparing intervention for use against CAUTIs and other medical device infections. eLife Sciences Publications, Ltd 2022-03-29 /pmc/articles/PMC8986317/ /pubmed/35348114 http://dx.doi.org/10.7554/eLife.75798 Text en © 2022, Andersen et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Microbiology and Infectious Disease
Andersen, Marissa Jeme
Fong, ChunKi
La Bella, Alyssa Ann
Molina, Jonathan Jesus
Molesan, Alex
Champion, Matthew M
Howell, Caitlin
Flores-Mireles, Ana L
Inhibiting host-protein deposition on urinary catheters reduces associated urinary tract infections
title Inhibiting host-protein deposition on urinary catheters reduces associated urinary tract infections
title_full Inhibiting host-protein deposition on urinary catheters reduces associated urinary tract infections
title_fullStr Inhibiting host-protein deposition on urinary catheters reduces associated urinary tract infections
title_full_unstemmed Inhibiting host-protein deposition on urinary catheters reduces associated urinary tract infections
title_short Inhibiting host-protein deposition on urinary catheters reduces associated urinary tract infections
title_sort inhibiting host-protein deposition on urinary catheters reduces associated urinary tract infections
topic Microbiology and Infectious Disease
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8986317/
https://www.ncbi.nlm.nih.gov/pubmed/35348114
http://dx.doi.org/10.7554/eLife.75798
work_keys_str_mv AT andersenmarissajeme inhibitinghostproteindepositiononurinarycathetersreducesassociatedurinarytractinfections
AT fongchunki inhibitinghostproteindepositiononurinarycathetersreducesassociatedurinarytractinfections
AT labellaalyssaann inhibitinghostproteindepositiononurinarycathetersreducesassociatedurinarytractinfections
AT molinajonathanjesus inhibitinghostproteindepositiononurinarycathetersreducesassociatedurinarytractinfections
AT molesanalex inhibitinghostproteindepositiononurinarycathetersreducesassociatedurinarytractinfections
AT championmatthewm inhibitinghostproteindepositiononurinarycathetersreducesassociatedurinarytractinfections
AT howellcaitlin inhibitinghostproteindepositiononurinarycathetersreducesassociatedurinarytractinfections
AT floresmirelesanal inhibitinghostproteindepositiononurinarycathetersreducesassociatedurinarytractinfections