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Acetyl-11-Keto-β-Boswellic Acid (AKBA) Prevents Lipopolysaccharide-Induced Inflammation and Cytotoxicity on H9C2 Cells

Acetyl-11-keto-beta-boswellic acid (AKBA), the major component of Boswellia serrata, exhibits anti-inflammatory activities. This in vitro study investigated the protective effects of AKBA against lipopolysaccharide (LPS)-induced cardiac dysfunction. In this study, the H9C2 cardiomyocytes were pretre...

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Autores principales: Taherzadeh, Danial, Baradaran Rahimi, Vafa, Amiri, Hamed, Ehtiati, Sajjad, Yahyazadeh, Roghayeh, Hashemy, Seyed Isaac, Askari, Vahid Reza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8986374/
https://www.ncbi.nlm.nih.gov/pubmed/35399644
http://dx.doi.org/10.1155/2022/2620710
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author Taherzadeh, Danial
Baradaran Rahimi, Vafa
Amiri, Hamed
Ehtiati, Sajjad
Yahyazadeh, Roghayeh
Hashemy, Seyed Isaac
Askari, Vahid Reza
author_facet Taherzadeh, Danial
Baradaran Rahimi, Vafa
Amiri, Hamed
Ehtiati, Sajjad
Yahyazadeh, Roghayeh
Hashemy, Seyed Isaac
Askari, Vahid Reza
author_sort Taherzadeh, Danial
collection PubMed
description Acetyl-11-keto-beta-boswellic acid (AKBA), the major component of Boswellia serrata, exhibits anti-inflammatory activities. This in vitro study investigated the protective effects of AKBA against lipopolysaccharide (LPS)-induced cardiac dysfunction. In this study, the H9C2 cardiomyocytes were pretreated with AKBA (2.5, 5, and 10 μM for 24 h), and then cotreated with LPS for another 24 h. The MTT assay, ELISA test kits, and quantitative real-time PCR analysis assessed the cell viability, levels of proinflammatory factors (IL-β, IL-6, TNF- α, and PGE2), and the gene expression of IL-β, IL-6, TNF- α, iNOS, and COX-2, respectively. The nitric oxide (NO) and thiol levels were also measured using a biochemical assay. The results indicated that LPS exposure markedly reduced cell viability and total thiol content, but increased the inflammatory cytokines, NO metabolites, and gene expression of proinflammatory mediators in H9C2 cells. AKBA pretreatment significantly altered the mentioned factors induced by LPS. Our results demonstrated that AKBA might be a promising therapeutic agent for treating sepsis-related cardiac dysfunction in the future.
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spelling pubmed-89863742022-04-07 Acetyl-11-Keto-β-Boswellic Acid (AKBA) Prevents Lipopolysaccharide-Induced Inflammation and Cytotoxicity on H9C2 Cells Taherzadeh, Danial Baradaran Rahimi, Vafa Amiri, Hamed Ehtiati, Sajjad Yahyazadeh, Roghayeh Hashemy, Seyed Isaac Askari, Vahid Reza Evid Based Complement Alternat Med Research Article Acetyl-11-keto-beta-boswellic acid (AKBA), the major component of Boswellia serrata, exhibits anti-inflammatory activities. This in vitro study investigated the protective effects of AKBA against lipopolysaccharide (LPS)-induced cardiac dysfunction. In this study, the H9C2 cardiomyocytes were pretreated with AKBA (2.5, 5, and 10 μM for 24 h), and then cotreated with LPS for another 24 h. The MTT assay, ELISA test kits, and quantitative real-time PCR analysis assessed the cell viability, levels of proinflammatory factors (IL-β, IL-6, TNF- α, and PGE2), and the gene expression of IL-β, IL-6, TNF- α, iNOS, and COX-2, respectively. The nitric oxide (NO) and thiol levels were also measured using a biochemical assay. The results indicated that LPS exposure markedly reduced cell viability and total thiol content, but increased the inflammatory cytokines, NO metabolites, and gene expression of proinflammatory mediators in H9C2 cells. AKBA pretreatment significantly altered the mentioned factors induced by LPS. Our results demonstrated that AKBA might be a promising therapeutic agent for treating sepsis-related cardiac dysfunction in the future. Hindawi 2022-03-30 /pmc/articles/PMC8986374/ /pubmed/35399644 http://dx.doi.org/10.1155/2022/2620710 Text en Copyright © 2022 Danial Taherzadeh et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Taherzadeh, Danial
Baradaran Rahimi, Vafa
Amiri, Hamed
Ehtiati, Sajjad
Yahyazadeh, Roghayeh
Hashemy, Seyed Isaac
Askari, Vahid Reza
Acetyl-11-Keto-β-Boswellic Acid (AKBA) Prevents Lipopolysaccharide-Induced Inflammation and Cytotoxicity on H9C2 Cells
title Acetyl-11-Keto-β-Boswellic Acid (AKBA) Prevents Lipopolysaccharide-Induced Inflammation and Cytotoxicity on H9C2 Cells
title_full Acetyl-11-Keto-β-Boswellic Acid (AKBA) Prevents Lipopolysaccharide-Induced Inflammation and Cytotoxicity on H9C2 Cells
title_fullStr Acetyl-11-Keto-β-Boswellic Acid (AKBA) Prevents Lipopolysaccharide-Induced Inflammation and Cytotoxicity on H9C2 Cells
title_full_unstemmed Acetyl-11-Keto-β-Boswellic Acid (AKBA) Prevents Lipopolysaccharide-Induced Inflammation and Cytotoxicity on H9C2 Cells
title_short Acetyl-11-Keto-β-Boswellic Acid (AKBA) Prevents Lipopolysaccharide-Induced Inflammation and Cytotoxicity on H9C2 Cells
title_sort acetyl-11-keto-β-boswellic acid (akba) prevents lipopolysaccharide-induced inflammation and cytotoxicity on h9c2 cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8986374/
https://www.ncbi.nlm.nih.gov/pubmed/35399644
http://dx.doi.org/10.1155/2022/2620710
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