Cargando…

Diversity of glpK Gene and Its Effect on Drug Sensitivity in Mycobacterium bovis

BACKGROUND: Glycerol kinase (glpK) is essential for the first step of glycerol catabolism in Mycobacterium tuberculosis. However, Mycobacterium bovis has been known to grow poorly in glycerol media because of a base insertion in the glpK gene. METHODS: We analyzed the glpK gene sequences of 60 clini...

Descripción completa

Detalles Bibliográficos
Autores principales: Dong, Yuhui, Ou, Xichao, Liu, Chunfa, Fan, Weixing, Zhao, Yanlin, Zhou, Xiangmei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8986483/
https://www.ncbi.nlm.nih.gov/pubmed/35401008
http://dx.doi.org/10.2147/IDR.S346724
_version_ 1784682554441334784
author Dong, Yuhui
Ou, Xichao
Liu, Chunfa
Fan, Weixing
Zhao, Yanlin
Zhou, Xiangmei
author_facet Dong, Yuhui
Ou, Xichao
Liu, Chunfa
Fan, Weixing
Zhao, Yanlin
Zhou, Xiangmei
author_sort Dong, Yuhui
collection PubMed
description BACKGROUND: Glycerol kinase (glpK) is essential for the first step of glycerol catabolism in Mycobacterium tuberculosis. However, Mycobacterium bovis has been known to grow poorly in glycerol media because of a base insertion in the glpK gene. METHODS: We analyzed the glpK gene sequences of 60 clinical M. bovis isolates, and determined the minimum inhibitory concentration of 14 drugs by microdilution method to evaluate the effect of frameshift mutations on drug sensitivity. The effect of M. bovis growth rate on its drug sensitivity was investigated using bacteria grown on glycerol or pyruvate. RESULTS: A total of 44 (73.33%) clinical M. bovis isolates have frameshift mutations in a homopolymeric tract of 7 cytosines in the glpK gene. 15.00% M. bovis isolates showed phenotypic drug resistance. Glycerol metabolism-deficient M. bovis showed reduced susceptibility to 9 out of 14 tested drugs. Mutations in the glpK gene can lead to impaired growth in glycerol-based media, while the minimal inhibitory concentration values of slow-growing M. bovis were higher. CONCLUSION: Mutations in the glpK gene can lead to slowed growth and reduced susceptibility to drugs in M. bovis, which may contribute to the emergence of drug-resistant M. bovis and pose a threat to human health owing to the zoonotic capacity of M. bovis.
format Online
Article
Text
id pubmed-8986483
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Dove
record_format MEDLINE/PubMed
spelling pubmed-89864832022-04-08 Diversity of glpK Gene and Its Effect on Drug Sensitivity in Mycobacterium bovis Dong, Yuhui Ou, Xichao Liu, Chunfa Fan, Weixing Zhao, Yanlin Zhou, Xiangmei Infect Drug Resist Original Research BACKGROUND: Glycerol kinase (glpK) is essential for the first step of glycerol catabolism in Mycobacterium tuberculosis. However, Mycobacterium bovis has been known to grow poorly in glycerol media because of a base insertion in the glpK gene. METHODS: We analyzed the glpK gene sequences of 60 clinical M. bovis isolates, and determined the minimum inhibitory concentration of 14 drugs by microdilution method to evaluate the effect of frameshift mutations on drug sensitivity. The effect of M. bovis growth rate on its drug sensitivity was investigated using bacteria grown on glycerol or pyruvate. RESULTS: A total of 44 (73.33%) clinical M. bovis isolates have frameshift mutations in a homopolymeric tract of 7 cytosines in the glpK gene. 15.00% M. bovis isolates showed phenotypic drug resistance. Glycerol metabolism-deficient M. bovis showed reduced susceptibility to 9 out of 14 tested drugs. Mutations in the glpK gene can lead to impaired growth in glycerol-based media, while the minimal inhibitory concentration values of slow-growing M. bovis were higher. CONCLUSION: Mutations in the glpK gene can lead to slowed growth and reduced susceptibility to drugs in M. bovis, which may contribute to the emergence of drug-resistant M. bovis and pose a threat to human health owing to the zoonotic capacity of M. bovis. Dove 2022-04-02 /pmc/articles/PMC8986483/ /pubmed/35401008 http://dx.doi.org/10.2147/IDR.S346724 Text en © 2022 Dong et al. https://creativecommons.org/licenses/by-nc/3.0/This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Dong, Yuhui
Ou, Xichao
Liu, Chunfa
Fan, Weixing
Zhao, Yanlin
Zhou, Xiangmei
Diversity of glpK Gene and Its Effect on Drug Sensitivity in Mycobacterium bovis
title Diversity of glpK Gene and Its Effect on Drug Sensitivity in Mycobacterium bovis
title_full Diversity of glpK Gene and Its Effect on Drug Sensitivity in Mycobacterium bovis
title_fullStr Diversity of glpK Gene and Its Effect on Drug Sensitivity in Mycobacterium bovis
title_full_unstemmed Diversity of glpK Gene and Its Effect on Drug Sensitivity in Mycobacterium bovis
title_short Diversity of glpK Gene and Its Effect on Drug Sensitivity in Mycobacterium bovis
title_sort diversity of glpk gene and its effect on drug sensitivity in mycobacterium bovis
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8986483/
https://www.ncbi.nlm.nih.gov/pubmed/35401008
http://dx.doi.org/10.2147/IDR.S346724
work_keys_str_mv AT dongyuhui diversityofglpkgeneanditseffectondrugsensitivityinmycobacteriumbovis
AT ouxichao diversityofglpkgeneanditseffectondrugsensitivityinmycobacteriumbovis
AT liuchunfa diversityofglpkgeneanditseffectondrugsensitivityinmycobacteriumbovis
AT fanweixing diversityofglpkgeneanditseffectondrugsensitivityinmycobacteriumbovis
AT zhaoyanlin diversityofglpkgeneanditseffectondrugsensitivityinmycobacteriumbovis
AT zhouxiangmei diversityofglpkgeneanditseffectondrugsensitivityinmycobacteriumbovis