Absolute measurement of cellular activities using photochromic single-fluorophore biosensors and intermittent quantification

Genetically-encoded biosensors based on a single fluorescent protein are widely used to visualize analyte levels or enzymatic activities in cells, though usually to monitor relative changes rather than absolute values. We report photochromism-enabled absolute quantification (PEAQ) biosensing, a meth...

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Detalles Bibliográficos
Autores principales: Bierbuesse, Franziska, Bourges, Anaïs C., Gielen, Vincent, Mönkemöller, Viola, Vandenberg, Wim, Shen, Yi, Hofkens, Johan, Vanden Berghe, Pieter, Campbell, Robert E., Moeyaert, Benjamien, Dedecker, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8986857/
https://www.ncbi.nlm.nih.gov/pubmed/35387971
http://dx.doi.org/10.1038/s41467-022-29508-w

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8986857/
https://www.ncbi.nlm.nih.gov/pubmed/35387971
http://dx.doi.org/10.1038/s41467-022-29508-w

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