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Absolute measurement of cellular activities using photochromic single-fluorophore biosensors and intermittent quantification
Genetically-encoded biosensors based on a single fluorescent protein are widely used to visualize analyte levels or enzymatic activities in cells, though usually to monitor relative changes rather than absolute values. We report photochromism-enabled absolute quantification (PEAQ) biosensing, a meth...
| Autores principales: | , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8986857/ https://www.ncbi.nlm.nih.gov/pubmed/35387971 http://dx.doi.org/10.1038/s41467-022-29508-w |