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Single-cell transcriptome analysis reveals aberrant stromal cells and heterogeneous endothelial cells in alcohol-induced osteonecrosis of the femoral head

Alcohol-induced osteonecrosis of the femoral head (ONFH) is a disabling disease with a high incidence and elusive pathogenesis. Here, we used single-cell RNA sequencing to explore the transcriptomic landscape of mid- and advanced-stage alcohol-induced ONFH. Cells derived from age-matched hip osteoar...

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Autores principales: Liao, Zheting, Jin, Yu, Chu, Yuhao, Wu, Hansen, Li, Xiaoyu, Deng, Zhonghao, Feng, Shuhao, Chen, Nachun, Luo, Ziheng, Zheng, Xiaoyong, Bao, Liangxiao, Xu, Yongqing, Tan, Hongbo, Zhao, Liang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8987047/
https://www.ncbi.nlm.nih.gov/pubmed/35388143
http://dx.doi.org/10.1038/s42003-022-03271-6
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author Liao, Zheting
Jin, Yu
Chu, Yuhao
Wu, Hansen
Li, Xiaoyu
Deng, Zhonghao
Feng, Shuhao
Chen, Nachun
Luo, Ziheng
Zheng, Xiaoyong
Bao, Liangxiao
Xu, Yongqing
Tan, Hongbo
Zhao, Liang
author_facet Liao, Zheting
Jin, Yu
Chu, Yuhao
Wu, Hansen
Li, Xiaoyu
Deng, Zhonghao
Feng, Shuhao
Chen, Nachun
Luo, Ziheng
Zheng, Xiaoyong
Bao, Liangxiao
Xu, Yongqing
Tan, Hongbo
Zhao, Liang
author_sort Liao, Zheting
collection PubMed
description Alcohol-induced osteonecrosis of the femoral head (ONFH) is a disabling disease with a high incidence and elusive pathogenesis. Here, we used single-cell RNA sequencing to explore the transcriptomic landscape of mid- and advanced-stage alcohol-induced ONFH. Cells derived from age-matched hip osteoarthritis and femoral neck fracture samples were used as control. Our bioinformatics analysis revealed the disorder of osteogenic-adipogenic differentiation of stromal cells in ONFH and altered regulons such as MEF2C and JUND. In addition, we reported that one of the endothelial cell clusters with ACKR1 expression exhibited strong chemotaxis and a weak angiogenic ability and expanded with disease progression. Furthermore, ligand-receptor-based cell-cell interaction analysis indicated that ACKR1+ endothelial cells might specifically communicate with stromal cells through the VISFATIN and SELE pathways, thus influencing stromal cell differentiation in ONFH. Overall, our data revealed single cell transcriptome characteristics in alcohol-induced ONFH, which may contribute to the further investigation of ONFH pathogenesis.
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spelling pubmed-89870472022-04-22 Single-cell transcriptome analysis reveals aberrant stromal cells and heterogeneous endothelial cells in alcohol-induced osteonecrosis of the femoral head Liao, Zheting Jin, Yu Chu, Yuhao Wu, Hansen Li, Xiaoyu Deng, Zhonghao Feng, Shuhao Chen, Nachun Luo, Ziheng Zheng, Xiaoyong Bao, Liangxiao Xu, Yongqing Tan, Hongbo Zhao, Liang Commun Biol Article Alcohol-induced osteonecrosis of the femoral head (ONFH) is a disabling disease with a high incidence and elusive pathogenesis. Here, we used single-cell RNA sequencing to explore the transcriptomic landscape of mid- and advanced-stage alcohol-induced ONFH. Cells derived from age-matched hip osteoarthritis and femoral neck fracture samples were used as control. Our bioinformatics analysis revealed the disorder of osteogenic-adipogenic differentiation of stromal cells in ONFH and altered regulons such as MEF2C and JUND. In addition, we reported that one of the endothelial cell clusters with ACKR1 expression exhibited strong chemotaxis and a weak angiogenic ability and expanded with disease progression. Furthermore, ligand-receptor-based cell-cell interaction analysis indicated that ACKR1+ endothelial cells might specifically communicate with stromal cells through the VISFATIN and SELE pathways, thus influencing stromal cell differentiation in ONFH. Overall, our data revealed single cell transcriptome characteristics in alcohol-induced ONFH, which may contribute to the further investigation of ONFH pathogenesis. Nature Publishing Group UK 2022-04-06 /pmc/articles/PMC8987047/ /pubmed/35388143 http://dx.doi.org/10.1038/s42003-022-03271-6 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Liao, Zheting
Jin, Yu
Chu, Yuhao
Wu, Hansen
Li, Xiaoyu
Deng, Zhonghao
Feng, Shuhao
Chen, Nachun
Luo, Ziheng
Zheng, Xiaoyong
Bao, Liangxiao
Xu, Yongqing
Tan, Hongbo
Zhao, Liang
Single-cell transcriptome analysis reveals aberrant stromal cells and heterogeneous endothelial cells in alcohol-induced osteonecrosis of the femoral head
title Single-cell transcriptome analysis reveals aberrant stromal cells and heterogeneous endothelial cells in alcohol-induced osteonecrosis of the femoral head
title_full Single-cell transcriptome analysis reveals aberrant stromal cells and heterogeneous endothelial cells in alcohol-induced osteonecrosis of the femoral head
title_fullStr Single-cell transcriptome analysis reveals aberrant stromal cells and heterogeneous endothelial cells in alcohol-induced osteonecrosis of the femoral head
title_full_unstemmed Single-cell transcriptome analysis reveals aberrant stromal cells and heterogeneous endothelial cells in alcohol-induced osteonecrosis of the femoral head
title_short Single-cell transcriptome analysis reveals aberrant stromal cells and heterogeneous endothelial cells in alcohol-induced osteonecrosis of the femoral head
title_sort single-cell transcriptome analysis reveals aberrant stromal cells and heterogeneous endothelial cells in alcohol-induced osteonecrosis of the femoral head
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8987047/
https://www.ncbi.nlm.nih.gov/pubmed/35388143
http://dx.doi.org/10.1038/s42003-022-03271-6
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