Cargando…
Photooxidation and Pentagalloyl Glucose Cross-Linking Improves the Performance of Decellularized Small-Diameter Vascular Xenograft In Vivo
Small-diameter vascular grafts have a significant need in peripheral vascular surgery and procedures of coronary artery bypass graft (CABG); however, autografts are not always available, synthetic grafts perform poorly, and allografts and xenografts dilate, calcify, and induce inflammation after imp...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8987116/ https://www.ncbi.nlm.nih.gov/pubmed/35402413 http://dx.doi.org/10.3389/fbioe.2022.816513 |
_version_ | 1784682667517673472 |
---|---|
author | Liu, Yuhong Chen, Chunyang Xie, Xinlong Yuan, Haoyong Tang, Zhenjie Qian, Tao Liu, Yalin Song, Mingzhe Liu, Sixi Lu, Ting Wu, Zhongshi |
author_facet | Liu, Yuhong Chen, Chunyang Xie, Xinlong Yuan, Haoyong Tang, Zhenjie Qian, Tao Liu, Yalin Song, Mingzhe Liu, Sixi Lu, Ting Wu, Zhongshi |
author_sort | Liu, Yuhong |
collection | PubMed |
description | Small-diameter vascular grafts have a significant need in peripheral vascular surgery and procedures of coronary artery bypass graft (CABG); however, autografts are not always available, synthetic grafts perform poorly, and allografts and xenografts dilate, calcify, and induce inflammation after implantation. We hypothesized that cross-linking of decellularized xenogeneic vascular grafts would improve the mechanical properties and biocompatibility and reduce inflammation, degradation, and calcification in vivo. To test this hypothesis, the bovine internal mammary artery (BIMA) was decellularized by detergents and ribozymes with sonication and perfusion. Photooxidation and pentagalloyl glucose (PGG) were used to cross-link the collagen and elastin fibers of decellularized xenografts. Modified grafts’ characteristics and biocompatibility were studied in vitro and in vivo; the grafts were implanted as transposition grafts in the subcutaneous of rats and the abdominal aorta of rabbits. The decellularized grafts were cross-linked by photooxidation and PGG, which improved the grafts’ biomechanical properties and biocompatibility, prevented elastic fibers from early degradation, and reduced inflammation and calcification in vivo. Short-term aortic implants in the rabbits showed collagen regeneration and differentiation of host smooth muscle cells. No occlusion and stenosis occurred due to remodeling and stabilization of the neointima. A good patency rate (100%) was maintained. Notably, implantation of non-treated grafts exhibited marked thrombosis, an inflammatory response, calcification, and elastin degeneration. Thus, photooxidation and PGG cross-linking are potential tools for improving grafts’ biological performance within decellularized small-diameter vascular xenografts. |
format | Online Article Text |
id | pubmed-8987116 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-89871162022-04-08 Photooxidation and Pentagalloyl Glucose Cross-Linking Improves the Performance of Decellularized Small-Diameter Vascular Xenograft In Vivo Liu, Yuhong Chen, Chunyang Xie, Xinlong Yuan, Haoyong Tang, Zhenjie Qian, Tao Liu, Yalin Song, Mingzhe Liu, Sixi Lu, Ting Wu, Zhongshi Front Bioeng Biotechnol Bioengineering and Biotechnology Small-diameter vascular grafts have a significant need in peripheral vascular surgery and procedures of coronary artery bypass graft (CABG); however, autografts are not always available, synthetic grafts perform poorly, and allografts and xenografts dilate, calcify, and induce inflammation after implantation. We hypothesized that cross-linking of decellularized xenogeneic vascular grafts would improve the mechanical properties and biocompatibility and reduce inflammation, degradation, and calcification in vivo. To test this hypothesis, the bovine internal mammary artery (BIMA) was decellularized by detergents and ribozymes with sonication and perfusion. Photooxidation and pentagalloyl glucose (PGG) were used to cross-link the collagen and elastin fibers of decellularized xenografts. Modified grafts’ characteristics and biocompatibility were studied in vitro and in vivo; the grafts were implanted as transposition grafts in the subcutaneous of rats and the abdominal aorta of rabbits. The decellularized grafts were cross-linked by photooxidation and PGG, which improved the grafts’ biomechanical properties and biocompatibility, prevented elastic fibers from early degradation, and reduced inflammation and calcification in vivo. Short-term aortic implants in the rabbits showed collagen regeneration and differentiation of host smooth muscle cells. No occlusion and stenosis occurred due to remodeling and stabilization of the neointima. A good patency rate (100%) was maintained. Notably, implantation of non-treated grafts exhibited marked thrombosis, an inflammatory response, calcification, and elastin degeneration. Thus, photooxidation and PGG cross-linking are potential tools for improving grafts’ biological performance within decellularized small-diameter vascular xenografts. Frontiers Media S.A. 2022-03-24 /pmc/articles/PMC8987116/ /pubmed/35402413 http://dx.doi.org/10.3389/fbioe.2022.816513 Text en Copyright © 2022 Liu, Chen, Xie, Yuan, Tang, Qian, Liu, Song, Liu, Lu and Wu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology Liu, Yuhong Chen, Chunyang Xie, Xinlong Yuan, Haoyong Tang, Zhenjie Qian, Tao Liu, Yalin Song, Mingzhe Liu, Sixi Lu, Ting Wu, Zhongshi Photooxidation and Pentagalloyl Glucose Cross-Linking Improves the Performance of Decellularized Small-Diameter Vascular Xenograft In Vivo |
title | Photooxidation and Pentagalloyl Glucose Cross-Linking Improves the Performance of Decellularized Small-Diameter Vascular Xenograft In Vivo
|
title_full | Photooxidation and Pentagalloyl Glucose Cross-Linking Improves the Performance of Decellularized Small-Diameter Vascular Xenograft In Vivo
|
title_fullStr | Photooxidation and Pentagalloyl Glucose Cross-Linking Improves the Performance of Decellularized Small-Diameter Vascular Xenograft In Vivo
|
title_full_unstemmed | Photooxidation and Pentagalloyl Glucose Cross-Linking Improves the Performance of Decellularized Small-Diameter Vascular Xenograft In Vivo
|
title_short | Photooxidation and Pentagalloyl Glucose Cross-Linking Improves the Performance of Decellularized Small-Diameter Vascular Xenograft In Vivo
|
title_sort | photooxidation and pentagalloyl glucose cross-linking improves the performance of decellularized small-diameter vascular xenograft in vivo |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8987116/ https://www.ncbi.nlm.nih.gov/pubmed/35402413 http://dx.doi.org/10.3389/fbioe.2022.816513 |
work_keys_str_mv | AT liuyuhong photooxidationandpentagalloylglucosecrosslinkingimprovestheperformanceofdecellularizedsmalldiametervascularxenograftinvivo AT chenchunyang photooxidationandpentagalloylglucosecrosslinkingimprovestheperformanceofdecellularizedsmalldiametervascularxenograftinvivo AT xiexinlong photooxidationandpentagalloylglucosecrosslinkingimprovestheperformanceofdecellularizedsmalldiametervascularxenograftinvivo AT yuanhaoyong photooxidationandpentagalloylglucosecrosslinkingimprovestheperformanceofdecellularizedsmalldiametervascularxenograftinvivo AT tangzhenjie photooxidationandpentagalloylglucosecrosslinkingimprovestheperformanceofdecellularizedsmalldiametervascularxenograftinvivo AT qiantao photooxidationandpentagalloylglucosecrosslinkingimprovestheperformanceofdecellularizedsmalldiametervascularxenograftinvivo AT liuyalin photooxidationandpentagalloylglucosecrosslinkingimprovestheperformanceofdecellularizedsmalldiametervascularxenograftinvivo AT songmingzhe photooxidationandpentagalloylglucosecrosslinkingimprovestheperformanceofdecellularizedsmalldiametervascularxenograftinvivo AT liusixi photooxidationandpentagalloylglucosecrosslinkingimprovestheperformanceofdecellularizedsmalldiametervascularxenograftinvivo AT luting photooxidationandpentagalloylglucosecrosslinkingimprovestheperformanceofdecellularizedsmalldiametervascularxenograftinvivo AT wuzhongshi photooxidationandpentagalloylglucosecrosslinkingimprovestheperformanceofdecellularizedsmalldiametervascularxenograftinvivo |