Cargando…
Transcriptomic Analysis of Liver Indicates Novel Vaccine to Porcine Reproductive and Respiratory Virus Promotes Homeostasis in T-Cell and Inflammatory Immune Responses Compared to a Commercial Vaccine in Pigs
One of the largest impediments for commercial swine production is the presence of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), a devastating RNA viral infection that is responsible for over $1 billion in loss in the U.S. annually. The challenge with combating PRRSV is a combination o...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8987227/ https://www.ncbi.nlm.nih.gov/pubmed/35400088 http://dx.doi.org/10.3389/fvets.2022.791034 |
_version_ | 1784682693842173952 |
---|---|
author | Fleming, Damarius S. Miller, Laura C. Li, Jiuyi Van Geelen, Albert Sang, Yongming |
author_facet | Fleming, Damarius S. Miller, Laura C. Li, Jiuyi Van Geelen, Albert Sang, Yongming |
author_sort | Fleming, Damarius S. |
collection | PubMed |
description | One of the largest impediments for commercial swine production is the presence of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), a devastating RNA viral infection that is responsible for over $1 billion in loss in the U.S. annually. The challenge with combating PRRSV is a combination of the effect of an extraordinary rate of mutation, the ability to infect macrophages, and subversion of host immune response through a series of actions leading to both immunomodulation and immune evasion. Currently there are a handful of commercial vaccines on the market that have been shown to be effective against homologous infections, but struggle against heterologous or mixed strain infections. However, vaccination is the current best strategy for combating PRRSV, making research into new vaccine technology key. To address these issues with PRRSV and host antiviral functions a novel modified-live vaccine (MLV) able to stimulate known antiviral interferons was created and examined for its ability to potentiate effective immunity and better protection. Here, we examine gene expression in the liver of pigs vaccinated with our novel vaccine, given the liver's large role in antiviral responses and vaccine metabolism. Our study indicated that pigs administered the novel vaccine experience homeostatic gene expression consistent with less inflammation and T-cell depletion risk than pigs administered the commercial vaccine. |
format | Online Article Text |
id | pubmed-8987227 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-89872272022-04-08 Transcriptomic Analysis of Liver Indicates Novel Vaccine to Porcine Reproductive and Respiratory Virus Promotes Homeostasis in T-Cell and Inflammatory Immune Responses Compared to a Commercial Vaccine in Pigs Fleming, Damarius S. Miller, Laura C. Li, Jiuyi Van Geelen, Albert Sang, Yongming Front Vet Sci Veterinary Science One of the largest impediments for commercial swine production is the presence of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), a devastating RNA viral infection that is responsible for over $1 billion in loss in the U.S. annually. The challenge with combating PRRSV is a combination of the effect of an extraordinary rate of mutation, the ability to infect macrophages, and subversion of host immune response through a series of actions leading to both immunomodulation and immune evasion. Currently there are a handful of commercial vaccines on the market that have been shown to be effective against homologous infections, but struggle against heterologous or mixed strain infections. However, vaccination is the current best strategy for combating PRRSV, making research into new vaccine technology key. To address these issues with PRRSV and host antiviral functions a novel modified-live vaccine (MLV) able to stimulate known antiviral interferons was created and examined for its ability to potentiate effective immunity and better protection. Here, we examine gene expression in the liver of pigs vaccinated with our novel vaccine, given the liver's large role in antiviral responses and vaccine metabolism. Our study indicated that pigs administered the novel vaccine experience homeostatic gene expression consistent with less inflammation and T-cell depletion risk than pigs administered the commercial vaccine. Frontiers Media S.A. 2022-03-24 /pmc/articles/PMC8987227/ /pubmed/35400088 http://dx.doi.org/10.3389/fvets.2022.791034 Text en Copyright © 2022 Fleming, Miller, Li, Van Geelen and Sang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Veterinary Science Fleming, Damarius S. Miller, Laura C. Li, Jiuyi Van Geelen, Albert Sang, Yongming Transcriptomic Analysis of Liver Indicates Novel Vaccine to Porcine Reproductive and Respiratory Virus Promotes Homeostasis in T-Cell and Inflammatory Immune Responses Compared to a Commercial Vaccine in Pigs |
title | Transcriptomic Analysis of Liver Indicates Novel Vaccine to Porcine Reproductive and Respiratory Virus Promotes Homeostasis in T-Cell and Inflammatory Immune Responses Compared to a Commercial Vaccine in Pigs |
title_full | Transcriptomic Analysis of Liver Indicates Novel Vaccine to Porcine Reproductive and Respiratory Virus Promotes Homeostasis in T-Cell and Inflammatory Immune Responses Compared to a Commercial Vaccine in Pigs |
title_fullStr | Transcriptomic Analysis of Liver Indicates Novel Vaccine to Porcine Reproductive and Respiratory Virus Promotes Homeostasis in T-Cell and Inflammatory Immune Responses Compared to a Commercial Vaccine in Pigs |
title_full_unstemmed | Transcriptomic Analysis of Liver Indicates Novel Vaccine to Porcine Reproductive and Respiratory Virus Promotes Homeostasis in T-Cell and Inflammatory Immune Responses Compared to a Commercial Vaccine in Pigs |
title_short | Transcriptomic Analysis of Liver Indicates Novel Vaccine to Porcine Reproductive and Respiratory Virus Promotes Homeostasis in T-Cell and Inflammatory Immune Responses Compared to a Commercial Vaccine in Pigs |
title_sort | transcriptomic analysis of liver indicates novel vaccine to porcine reproductive and respiratory virus promotes homeostasis in t-cell and inflammatory immune responses compared to a commercial vaccine in pigs |
topic | Veterinary Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8987227/ https://www.ncbi.nlm.nih.gov/pubmed/35400088 http://dx.doi.org/10.3389/fvets.2022.791034 |
work_keys_str_mv | AT flemingdamariuss transcriptomicanalysisofliverindicatesnovelvaccinetoporcinereproductiveandrespiratoryviruspromoteshomeostasisintcellandinflammatoryimmuneresponsescomparedtoacommercialvaccineinpigs AT millerlaurac transcriptomicanalysisofliverindicatesnovelvaccinetoporcinereproductiveandrespiratoryviruspromoteshomeostasisintcellandinflammatoryimmuneresponsescomparedtoacommercialvaccineinpigs AT lijiuyi transcriptomicanalysisofliverindicatesnovelvaccinetoporcinereproductiveandrespiratoryviruspromoteshomeostasisintcellandinflammatoryimmuneresponsescomparedtoacommercialvaccineinpigs AT vangeelenalbert transcriptomicanalysisofliverindicatesnovelvaccinetoporcinereproductiveandrespiratoryviruspromoteshomeostasisintcellandinflammatoryimmuneresponsescomparedtoacommercialvaccineinpigs AT sangyongming transcriptomicanalysisofliverindicatesnovelvaccinetoporcinereproductiveandrespiratoryviruspromoteshomeostasisintcellandinflammatoryimmuneresponsescomparedtoacommercialvaccineinpigs |