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Impact of Pre-Anthesis Drought Stress on Physiology, Yield-Related Traits, and Drought-Responsive Genes in Green Super Rice
Optimum soil water availability is vital for maximum yield production in rice which is challenged by increasing spells of drought. The reproductive stage drought is among the main limiting factors leading to the drastic reduction in grain yield. The objective of this study was to investigate the mol...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8987348/ https://www.ncbi.nlm.nih.gov/pubmed/35401708 http://dx.doi.org/10.3389/fgene.2022.832542 |
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author | Ahmad, Hassaan Zafar, Syed Adeel Naeem, Muhammad Kashif Shokat, Sajid Inam, Safeena Rehman, Malik Attique ur Naveed, Shahzad Amir Xu, Jianlong Li, Zhikang Ali, Ghulam Muhammad Khan, Muhammad Ramzan |
author_facet | Ahmad, Hassaan Zafar, Syed Adeel Naeem, Muhammad Kashif Shokat, Sajid Inam, Safeena Rehman, Malik Attique ur Naveed, Shahzad Amir Xu, Jianlong Li, Zhikang Ali, Ghulam Muhammad Khan, Muhammad Ramzan |
author_sort | Ahmad, Hassaan |
collection | PubMed |
description | Optimum soil water availability is vital for maximum yield production in rice which is challenged by increasing spells of drought. The reproductive stage drought is among the main limiting factors leading to the drastic reduction in grain yield. The objective of this study was to investigate the molecular and morphophysiological responses of pre-anthesis stage drought stress in green super rice. The study assessed the performance of 26 rice lines under irrigated and drought conditions. Irrigated treatment was allowed to grow normally, while drought stress was imposed for 30 days at the pre-anthesis stage. Three important physiological traits including pollen fertility percentage (PFP), cell membrane stability (CMS), and normalized difference vegetative index (NDVI) were recorded at anthesis stage during the last week of drought stress. Agronomic traits of economic importance including grain yield were recorded at maturity stage. The analysis of variance demonstrated significant variation among the genotypes for most of the studied traits. Correlation and principal component analyses demonstrated highly significant associations of particular agronomic traits with grain yield, and genetic diversity among genotypes, respectively. Our study demonstrated a higher drought tolerance potential of GSR lines compared with local cultivars, mainly by higher pollen viability, plant biomass, CMS, and harvest index under drought. In addition, the molecular basis of drought tolerance in GSR lines was related to upregulation of certain drought-responsive genes including OsSADRI, OsDSM1, OsDT11, but not the DREB genes. Our study identified novel drought-responsive genes (LOC_Os11g36190, LOC_Os12g04500, LOC_Os12g26290, and LOC_Os02g11960) that could be further characterized using reverse genetics to be utilized in molecular breeding for drought tolerance. |
format | Online Article Text |
id | pubmed-8987348 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-89873482022-04-08 Impact of Pre-Anthesis Drought Stress on Physiology, Yield-Related Traits, and Drought-Responsive Genes in Green Super Rice Ahmad, Hassaan Zafar, Syed Adeel Naeem, Muhammad Kashif Shokat, Sajid Inam, Safeena Rehman, Malik Attique ur Naveed, Shahzad Amir Xu, Jianlong Li, Zhikang Ali, Ghulam Muhammad Khan, Muhammad Ramzan Front Genet Genetics Optimum soil water availability is vital for maximum yield production in rice which is challenged by increasing spells of drought. The reproductive stage drought is among the main limiting factors leading to the drastic reduction in grain yield. The objective of this study was to investigate the molecular and morphophysiological responses of pre-anthesis stage drought stress in green super rice. The study assessed the performance of 26 rice lines under irrigated and drought conditions. Irrigated treatment was allowed to grow normally, while drought stress was imposed for 30 days at the pre-anthesis stage. Three important physiological traits including pollen fertility percentage (PFP), cell membrane stability (CMS), and normalized difference vegetative index (NDVI) were recorded at anthesis stage during the last week of drought stress. Agronomic traits of economic importance including grain yield were recorded at maturity stage. The analysis of variance demonstrated significant variation among the genotypes for most of the studied traits. Correlation and principal component analyses demonstrated highly significant associations of particular agronomic traits with grain yield, and genetic diversity among genotypes, respectively. Our study demonstrated a higher drought tolerance potential of GSR lines compared with local cultivars, mainly by higher pollen viability, plant biomass, CMS, and harvest index under drought. In addition, the molecular basis of drought tolerance in GSR lines was related to upregulation of certain drought-responsive genes including OsSADRI, OsDSM1, OsDT11, but not the DREB genes. Our study identified novel drought-responsive genes (LOC_Os11g36190, LOC_Os12g04500, LOC_Os12g26290, and LOC_Os02g11960) that could be further characterized using reverse genetics to be utilized in molecular breeding for drought tolerance. Frontiers Media S.A. 2022-03-24 /pmc/articles/PMC8987348/ /pubmed/35401708 http://dx.doi.org/10.3389/fgene.2022.832542 Text en Copyright © 2022 Ahmad, Zafar, Naeem, Shokat, Inam, Rehman, Naveed, Xu, Li, Ali and Khan. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Genetics Ahmad, Hassaan Zafar, Syed Adeel Naeem, Muhammad Kashif Shokat, Sajid Inam, Safeena Rehman, Malik Attique ur Naveed, Shahzad Amir Xu, Jianlong Li, Zhikang Ali, Ghulam Muhammad Khan, Muhammad Ramzan Impact of Pre-Anthesis Drought Stress on Physiology, Yield-Related Traits, and Drought-Responsive Genes in Green Super Rice |
title | Impact of Pre-Anthesis Drought Stress on Physiology, Yield-Related Traits, and Drought-Responsive Genes in Green Super Rice |
title_full | Impact of Pre-Anthesis Drought Stress on Physiology, Yield-Related Traits, and Drought-Responsive Genes in Green Super Rice |
title_fullStr | Impact of Pre-Anthesis Drought Stress on Physiology, Yield-Related Traits, and Drought-Responsive Genes in Green Super Rice |
title_full_unstemmed | Impact of Pre-Anthesis Drought Stress on Physiology, Yield-Related Traits, and Drought-Responsive Genes in Green Super Rice |
title_short | Impact of Pre-Anthesis Drought Stress on Physiology, Yield-Related Traits, and Drought-Responsive Genes in Green Super Rice |
title_sort | impact of pre-anthesis drought stress on physiology, yield-related traits, and drought-responsive genes in green super rice |
topic | Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8987348/ https://www.ncbi.nlm.nih.gov/pubmed/35401708 http://dx.doi.org/10.3389/fgene.2022.832542 |
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