Cargando…

Preclinical Study of Biphasic Asymmetric Pulsed Field Ablation

Pulsed field ablation (PFA) is a novel method of pulmonary venous isolation in atrial fibrillation ablation and is featured by tissue-selective ablation. Isolation is achieved via the application of high-voltage microsecond pulses that create irreversible perforations in cell membranes (i.e., electr...

Descripción completa

Detalles Bibliográficos
Autores principales: Bi, Shengyu, Jia, Fenglin, Lv, Chang, He, Qiang, Xu, Xinyu, Xue, Zhixiao, Su, Siying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8987372/
https://www.ncbi.nlm.nih.gov/pubmed/35402543
http://dx.doi.org/10.3389/fcvm.2022.859480
_version_ 1784682726704545792
author Bi, Shengyu
Jia, Fenglin
Lv, Chang
He, Qiang
Xu, Xinyu
Xue, Zhixiao
Su, Siying
author_facet Bi, Shengyu
Jia, Fenglin
Lv, Chang
He, Qiang
Xu, Xinyu
Xue, Zhixiao
Su, Siying
author_sort Bi, Shengyu
collection PubMed
description Pulsed field ablation (PFA) is a novel method of pulmonary venous isolation in atrial fibrillation ablation and is featured by tissue-selective ablation. Isolation is achieved via the application of high-voltage microsecond pulses that create irreversible perforations in cell membranes (i.e., electroporation). We proposed a new biphasic asymmetric pulse mode and verified the lesion persistence and safety of this mode for pulmonary vein ostia ablation in preclinical studies. We found that biphasic asymmetric pulses can effectively reduce muscle contractions and drop ablation threshold. In the electroanatomic mapping, the ablation site showed a continuous low potential area, and the atrium was not captured after 30 days of pacing. Pathological staining showed that cardiomyocytes in the ablation area were replaced by fibroblasts and there was no damage outside the ablation zone. Our results show that pulmonary venous isolation using the biphasic asymmetric discharge mode is safe, durable, effective, and causes no damage to other tissues.
format Online
Article
Text
id pubmed-8987372
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-89873722022-04-08 Preclinical Study of Biphasic Asymmetric Pulsed Field Ablation Bi, Shengyu Jia, Fenglin Lv, Chang He, Qiang Xu, Xinyu Xue, Zhixiao Su, Siying Front Cardiovasc Med Cardiovascular Medicine Pulsed field ablation (PFA) is a novel method of pulmonary venous isolation in atrial fibrillation ablation and is featured by tissue-selective ablation. Isolation is achieved via the application of high-voltage microsecond pulses that create irreversible perforations in cell membranes (i.e., electroporation). We proposed a new biphasic asymmetric pulse mode and verified the lesion persistence and safety of this mode for pulmonary vein ostia ablation in preclinical studies. We found that biphasic asymmetric pulses can effectively reduce muscle contractions and drop ablation threshold. In the electroanatomic mapping, the ablation site showed a continuous low potential area, and the atrium was not captured after 30 days of pacing. Pathological staining showed that cardiomyocytes in the ablation area were replaced by fibroblasts and there was no damage outside the ablation zone. Our results show that pulmonary venous isolation using the biphasic asymmetric discharge mode is safe, durable, effective, and causes no damage to other tissues. Frontiers Media S.A. 2022-03-24 /pmc/articles/PMC8987372/ /pubmed/35402543 http://dx.doi.org/10.3389/fcvm.2022.859480 Text en Copyright © 2022 Bi, Jia, Lv, He, Xu, Xue and Su. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cardiovascular Medicine
Bi, Shengyu
Jia, Fenglin
Lv, Chang
He, Qiang
Xu, Xinyu
Xue, Zhixiao
Su, Siying
Preclinical Study of Biphasic Asymmetric Pulsed Field Ablation
title Preclinical Study of Biphasic Asymmetric Pulsed Field Ablation
title_full Preclinical Study of Biphasic Asymmetric Pulsed Field Ablation
title_fullStr Preclinical Study of Biphasic Asymmetric Pulsed Field Ablation
title_full_unstemmed Preclinical Study of Biphasic Asymmetric Pulsed Field Ablation
title_short Preclinical Study of Biphasic Asymmetric Pulsed Field Ablation
title_sort preclinical study of biphasic asymmetric pulsed field ablation
topic Cardiovascular Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8987372/
https://www.ncbi.nlm.nih.gov/pubmed/35402543
http://dx.doi.org/10.3389/fcvm.2022.859480
work_keys_str_mv AT bishengyu preclinicalstudyofbiphasicasymmetricpulsedfieldablation
AT jiafenglin preclinicalstudyofbiphasicasymmetricpulsedfieldablation
AT lvchang preclinicalstudyofbiphasicasymmetricpulsedfieldablation
AT heqiang preclinicalstudyofbiphasicasymmetricpulsedfieldablation
AT xuxinyu preclinicalstudyofbiphasicasymmetricpulsedfieldablation
AT xuezhixiao preclinicalstudyofbiphasicasymmetricpulsedfieldablation
AT susiying preclinicalstudyofbiphasicasymmetricpulsedfieldablation