Generation of human embryonic stem cell-derived lung organoids

This protocol describes how to generate lung organoids from human embryonic stem cells. Lung organoids form by self-assembly in Matrigel and contain lung epithelial cell types. The protocol presented in this study is simple and only uses 6 cytokines or small molecules. This protocol provides a promi...

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Detalles Bibliográficos
Autores principales: Han, Le, Zhao, Shanshan, Yu, Fuxun, Rong, Zhili, Lin, Ying, Chen, Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8987391/
https://www.ncbi.nlm.nih.gov/pubmed/35403011
http://dx.doi.org/10.1016/j.xpro.2022.101270
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author Han, Le
Zhao, Shanshan
Yu, Fuxun
Rong, Zhili
Lin, Ying
Chen, Yong
author_facet Han, Le
Zhao, Shanshan
Yu, Fuxun
Rong, Zhili
Lin, Ying
Chen, Yong
author_sort Han, Le
collection PubMed
description This protocol describes how to generate lung organoids from human embryonic stem cells. Lung organoids form by self-assembly in Matrigel and contain lung epithelial cell types. The protocol presented in this study is simple and only uses 6 cytokines or small molecules. This protocol provides a promising tool to study human lung development, drug screening, regeneration, and disease modeling in vitro. For complete details on the use and execution of this protocol, please refer to Chen et al. (2018).
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spelling pubmed-89873912022-04-08 Generation of human embryonic stem cell-derived lung organoids Han, Le Zhao, Shanshan Yu, Fuxun Rong, Zhili Lin, Ying Chen, Yong STAR Protoc Protocol This protocol describes how to generate lung organoids from human embryonic stem cells. Lung organoids form by self-assembly in Matrigel and contain lung epithelial cell types. The protocol presented in this study is simple and only uses 6 cytokines or small molecules. This protocol provides a promising tool to study human lung development, drug screening, regeneration, and disease modeling in vitro. For complete details on the use and execution of this protocol, please refer to Chen et al. (2018). Elsevier 2022-04-04 /pmc/articles/PMC8987391/ /pubmed/35403011 http://dx.doi.org/10.1016/j.xpro.2022.101270 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Han, Le
Zhao, Shanshan
Yu, Fuxun
Rong, Zhili
Lin, Ying
Chen, Yong
Generation of human embryonic stem cell-derived lung organoids
title Generation of human embryonic stem cell-derived lung organoids
title_full Generation of human embryonic stem cell-derived lung organoids
title_fullStr Generation of human embryonic stem cell-derived lung organoids
title_full_unstemmed Generation of human embryonic stem cell-derived lung organoids
title_short Generation of human embryonic stem cell-derived lung organoids
title_sort generation of human embryonic stem cell-derived lung organoids
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8987391/
https://www.ncbi.nlm.nih.gov/pubmed/35403011
http://dx.doi.org/10.1016/j.xpro.2022.101270
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