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MiRAGDB: A Knowledgebase of RAG Regulators

RAG1 and RAG2 genes generate diversity in immunoglobulin and TCR genes by initiating the process of V-D-J recombination. RAGs recognize specific sequences (heptamer-nonamer) to generate a diversity of immunoglobulins. RAG expression is limited to early B and T cell developmental stages. Aberrant exp...

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Autores principales: Desai, Sagar Sanjiv, Whadgar, Saurabh, Raghavan, Sathees C., Choudhary, Bibha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8987502/
https://www.ncbi.nlm.nih.gov/pubmed/35401578
http://dx.doi.org/10.3389/fimmu.2022.863110
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author Desai, Sagar Sanjiv
Whadgar, Saurabh
Raghavan, Sathees C.
Choudhary, Bibha
author_facet Desai, Sagar Sanjiv
Whadgar, Saurabh
Raghavan, Sathees C.
Choudhary, Bibha
author_sort Desai, Sagar Sanjiv
collection PubMed
description RAG1 and RAG2 genes generate diversity in immunoglobulin and TCR genes by initiating the process of V-D-J recombination. RAGs recognize specific sequences (heptamer-nonamer) to generate a diversity of immunoglobulins. RAG expression is limited to early B and T cell developmental stages. Aberrant expression of RAG can lead to double strand breaks and translocations as observed in leukemia and lymphoma. The expression of RAG is tightly regulated at the transcriptional and posttranscriptional levels. MicroRNAs (miRNAs) are small non-coding RNAs that are involved in the post-transcriptional regulation of gene expression. This study aimed to identify and catalog RAG regulation by miRNA during normal development and cancer. NGS data from normal B-cell and T-cell developmental stages and blood cancer samples have been analyzed for the expression of miRNAs against RAG1 (1,173 against human RAG1 and 749 against mouse RAG1). The analyzed data has been organized to retrieve the miRNA and mRNA expression of various RAG regulators (10 transcription factors and interacting partners) in normal and diseased states. The database allows users to navigate through the human and mouse RAG regulators, visualize and plot expression. miRAGDB is freely available and can be accessed at http://52.4.112.252/shiny/miragdb/.
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spelling pubmed-89875022022-04-08 MiRAGDB: A Knowledgebase of RAG Regulators Desai, Sagar Sanjiv Whadgar, Saurabh Raghavan, Sathees C. Choudhary, Bibha Front Immunol Immunology RAG1 and RAG2 genes generate diversity in immunoglobulin and TCR genes by initiating the process of V-D-J recombination. RAGs recognize specific sequences (heptamer-nonamer) to generate a diversity of immunoglobulins. RAG expression is limited to early B and T cell developmental stages. Aberrant expression of RAG can lead to double strand breaks and translocations as observed in leukemia and lymphoma. The expression of RAG is tightly regulated at the transcriptional and posttranscriptional levels. MicroRNAs (miRNAs) are small non-coding RNAs that are involved in the post-transcriptional regulation of gene expression. This study aimed to identify and catalog RAG regulation by miRNA during normal development and cancer. NGS data from normal B-cell and T-cell developmental stages and blood cancer samples have been analyzed for the expression of miRNAs against RAG1 (1,173 against human RAG1 and 749 against mouse RAG1). The analyzed data has been organized to retrieve the miRNA and mRNA expression of various RAG regulators (10 transcription factors and interacting partners) in normal and diseased states. The database allows users to navigate through the human and mouse RAG regulators, visualize and plot expression. miRAGDB is freely available and can be accessed at http://52.4.112.252/shiny/miragdb/. Frontiers Media S.A. 2022-03-24 /pmc/articles/PMC8987502/ /pubmed/35401578 http://dx.doi.org/10.3389/fimmu.2022.863110 Text en Copyright © 2022 Desai, Whadgar, Raghavan and Choudhary https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Desai, Sagar Sanjiv
Whadgar, Saurabh
Raghavan, Sathees C.
Choudhary, Bibha
MiRAGDB: A Knowledgebase of RAG Regulators
title MiRAGDB: A Knowledgebase of RAG Regulators
title_full MiRAGDB: A Knowledgebase of RAG Regulators
title_fullStr MiRAGDB: A Knowledgebase of RAG Regulators
title_full_unstemmed MiRAGDB: A Knowledgebase of RAG Regulators
title_short MiRAGDB: A Knowledgebase of RAG Regulators
title_sort miragdb: a knowledgebase of rag regulators
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8987502/
https://www.ncbi.nlm.nih.gov/pubmed/35401578
http://dx.doi.org/10.3389/fimmu.2022.863110
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