Cargando…

Intravital Multiphoton Microscopy as a Tool for Studying Renal Physiology, Pathophysiology and Therapeutics

Intravital multiphoton microscopy has empowered investigators to study dynamic cell and subcellular processes in vivo within normal and disease organs. Advances in hardware, software, optics, transgenics and fluorescent probe design and development have enabled new quantitative approaches to create...

Descripción completa

Detalles Bibliográficos
Autores principales: Molitoris, Bruce A., Sandoval, Ruben M., Wagner, Mark C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8988037/
https://www.ncbi.nlm.nih.gov/pubmed/35399274
http://dx.doi.org/10.3389/fphys.2022.827280
_version_ 1784682873953976320
author Molitoris, Bruce A.
Sandoval, Ruben M.
Wagner, Mark C.
author_facet Molitoris, Bruce A.
Sandoval, Ruben M.
Wagner, Mark C.
author_sort Molitoris, Bruce A.
collection PubMed
description Intravital multiphoton microscopy has empowered investigators to study dynamic cell and subcellular processes in vivo within normal and disease organs. Advances in hardware, software, optics, transgenics and fluorescent probe design and development have enabled new quantitative approaches to create a disruptive technology pioneering advances in understanding of normal biology, disease pathophysiology and therapies. Offering superior spatial and temporal resolution with high sensitivity, investigators can follow multiple processes simultaneously and observe complex interactions between different cell types, intracellular organelles, proteins and track molecules for cellular uptake, intracellular trafficking, and metabolism in a cell specific fashion. The technique has been utilized in the kidney to quantify multiple dynamic processes including capillary flow, permeability, glomerular function, proximal tubule processes and determine the effects of diseases and therapeutic mechanisms. Limitations include the depth of tissue penetration with loss of sensitivity and resolution due to scattered emitted light. Tissue clearing technology has virtually eliminated penetration issues for fixed tissue studies. Use of multiphoton microscopy in preclinical animal models offers distinct advantages resulting in new insights into physiologic processes and the pathophysiology and treatment of diseases.
format Online
Article
Text
id pubmed-8988037
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-89880372022-04-08 Intravital Multiphoton Microscopy as a Tool for Studying Renal Physiology, Pathophysiology and Therapeutics Molitoris, Bruce A. Sandoval, Ruben M. Wagner, Mark C. Front Physiol Physiology Intravital multiphoton microscopy has empowered investigators to study dynamic cell and subcellular processes in vivo within normal and disease organs. Advances in hardware, software, optics, transgenics and fluorescent probe design and development have enabled new quantitative approaches to create a disruptive technology pioneering advances in understanding of normal biology, disease pathophysiology and therapies. Offering superior spatial and temporal resolution with high sensitivity, investigators can follow multiple processes simultaneously and observe complex interactions between different cell types, intracellular organelles, proteins and track molecules for cellular uptake, intracellular trafficking, and metabolism in a cell specific fashion. The technique has been utilized in the kidney to quantify multiple dynamic processes including capillary flow, permeability, glomerular function, proximal tubule processes and determine the effects of diseases and therapeutic mechanisms. Limitations include the depth of tissue penetration with loss of sensitivity and resolution due to scattered emitted light. Tissue clearing technology has virtually eliminated penetration issues for fixed tissue studies. Use of multiphoton microscopy in preclinical animal models offers distinct advantages resulting in new insights into physiologic processes and the pathophysiology and treatment of diseases. Frontiers Media S.A. 2022-03-24 /pmc/articles/PMC8988037/ /pubmed/35399274 http://dx.doi.org/10.3389/fphys.2022.827280 Text en Copyright © 2022 Molitoris, Sandoval and Wagner. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Molitoris, Bruce A.
Sandoval, Ruben M.
Wagner, Mark C.
Intravital Multiphoton Microscopy as a Tool for Studying Renal Physiology, Pathophysiology and Therapeutics
title Intravital Multiphoton Microscopy as a Tool for Studying Renal Physiology, Pathophysiology and Therapeutics
title_full Intravital Multiphoton Microscopy as a Tool for Studying Renal Physiology, Pathophysiology and Therapeutics
title_fullStr Intravital Multiphoton Microscopy as a Tool for Studying Renal Physiology, Pathophysiology and Therapeutics
title_full_unstemmed Intravital Multiphoton Microscopy as a Tool for Studying Renal Physiology, Pathophysiology and Therapeutics
title_short Intravital Multiphoton Microscopy as a Tool for Studying Renal Physiology, Pathophysiology and Therapeutics
title_sort intravital multiphoton microscopy as a tool for studying renal physiology, pathophysiology and therapeutics
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8988037/
https://www.ncbi.nlm.nih.gov/pubmed/35399274
http://dx.doi.org/10.3389/fphys.2022.827280
work_keys_str_mv AT molitorisbrucea intravitalmultiphotonmicroscopyasatoolforstudyingrenalphysiologypathophysiologyandtherapeutics
AT sandovalrubenm intravitalmultiphotonmicroscopyasatoolforstudyingrenalphysiologypathophysiologyandtherapeutics
AT wagnermarkc intravitalmultiphotonmicroscopyasatoolforstudyingrenalphysiologypathophysiologyandtherapeutics