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Comparison of Microbial Community and Metabolites in Four Stomach Compartments of Myostatin-Gene-Edited and Non-edited Cattle

Myostatin (MSTN), a major negative regulator of skeletal muscle mass and an endocrine factor, can regulate the metabolism of various organisms. Inhibition of the MSTN gene can improve meat production from livestock. Rumen microorganisms are associated with production and health traits of cattle, but...

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Autores principales: Zhou, Xinyu, Gu, Mingjuan, Zhu, Lin, Wu, Di, Yang, Miaomiao, Gao, Yajie, Wang, Xueqiao, Bai, Chunling, Wei, Zhuying, Yang, Lei, Li, Guangpeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8988179/
https://www.ncbi.nlm.nih.gov/pubmed/35401485
http://dx.doi.org/10.3389/fmicb.2022.844962
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author Zhou, Xinyu
Gu, Mingjuan
Zhu, Lin
Wu, Di
Yang, Miaomiao
Gao, Yajie
Wang, Xueqiao
Bai, Chunling
Wei, Zhuying
Yang, Lei
Li, Guangpeng
author_facet Zhou, Xinyu
Gu, Mingjuan
Zhu, Lin
Wu, Di
Yang, Miaomiao
Gao, Yajie
Wang, Xueqiao
Bai, Chunling
Wei, Zhuying
Yang, Lei
Li, Guangpeng
author_sort Zhou, Xinyu
collection PubMed
description Myostatin (MSTN), a major negative regulator of skeletal muscle mass and an endocrine factor, can regulate the metabolism of various organisms. Inhibition of the MSTN gene can improve meat production from livestock. Rumen microorganisms are associated with production and health traits of cattle, but changes in the microbial composition and metabolome in the four stomach compartments of MSTN gene–edited cattle have not previously been studied. Our results indicated that microbial diversity and dominant bacteria in the four stomach compartments were very similar between MSTN gene–edited and wild-type (WT) cattle. The microbiota composition was significantly different between MSTN gene–edited and WT cattle. Our results show that the relative abundance of the phylum Proteobacteria in the reticulum of MSTN gene–edited cattle was lower than that of WT cattle, whereas the relative abundance of the genus Prevotella in the omasum of MSTN gene–edited cattle was significantly higher than that of WT cattle. Metabolomics analysis revealed that the intensity of L-proline and acetic acid was significantly different in the rumen, reticulum, and abomasum between the two types of cattle. Meanwhile, pathway topology analysis indicated that the differential metabolites were predominantly involved in arginine biosynthesis and glutamate metabolism in the rumen, reticulum, and omasum but were mainly involved in pyruvate metabolism and glycolysis/gluconeogenesis in the abomasum. Spearman correlation network analysis further demonstrated that there was a significant correlation between microflora composition and metabolic pathways. These findings provide clues for studying nutrient digestion and absorption ability of MSTN gene–edited cattle.
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spelling pubmed-89881792022-04-08 Comparison of Microbial Community and Metabolites in Four Stomach Compartments of Myostatin-Gene-Edited and Non-edited Cattle Zhou, Xinyu Gu, Mingjuan Zhu, Lin Wu, Di Yang, Miaomiao Gao, Yajie Wang, Xueqiao Bai, Chunling Wei, Zhuying Yang, Lei Li, Guangpeng Front Microbiol Microbiology Myostatin (MSTN), a major negative regulator of skeletal muscle mass and an endocrine factor, can regulate the metabolism of various organisms. Inhibition of the MSTN gene can improve meat production from livestock. Rumen microorganisms are associated with production and health traits of cattle, but changes in the microbial composition and metabolome in the four stomach compartments of MSTN gene–edited cattle have not previously been studied. Our results indicated that microbial diversity and dominant bacteria in the four stomach compartments were very similar between MSTN gene–edited and wild-type (WT) cattle. The microbiota composition was significantly different between MSTN gene–edited and WT cattle. Our results show that the relative abundance of the phylum Proteobacteria in the reticulum of MSTN gene–edited cattle was lower than that of WT cattle, whereas the relative abundance of the genus Prevotella in the omasum of MSTN gene–edited cattle was significantly higher than that of WT cattle. Metabolomics analysis revealed that the intensity of L-proline and acetic acid was significantly different in the rumen, reticulum, and abomasum between the two types of cattle. Meanwhile, pathway topology analysis indicated that the differential metabolites were predominantly involved in arginine biosynthesis and glutamate metabolism in the rumen, reticulum, and omasum but were mainly involved in pyruvate metabolism and glycolysis/gluconeogenesis in the abomasum. Spearman correlation network analysis further demonstrated that there was a significant correlation between microflora composition and metabolic pathways. These findings provide clues for studying nutrient digestion and absorption ability of MSTN gene–edited cattle. Frontiers Media S.A. 2022-03-24 /pmc/articles/PMC8988179/ /pubmed/35401485 http://dx.doi.org/10.3389/fmicb.2022.844962 Text en Copyright © 2022 Zhou, Gu, Zhu, Wu, Yang, Gao, Wang, Bai, Wei, Yang and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Zhou, Xinyu
Gu, Mingjuan
Zhu, Lin
Wu, Di
Yang, Miaomiao
Gao, Yajie
Wang, Xueqiao
Bai, Chunling
Wei, Zhuying
Yang, Lei
Li, Guangpeng
Comparison of Microbial Community and Metabolites in Four Stomach Compartments of Myostatin-Gene-Edited and Non-edited Cattle
title Comparison of Microbial Community and Metabolites in Four Stomach Compartments of Myostatin-Gene-Edited and Non-edited Cattle
title_full Comparison of Microbial Community and Metabolites in Four Stomach Compartments of Myostatin-Gene-Edited and Non-edited Cattle
title_fullStr Comparison of Microbial Community and Metabolites in Four Stomach Compartments of Myostatin-Gene-Edited and Non-edited Cattle
title_full_unstemmed Comparison of Microbial Community and Metabolites in Four Stomach Compartments of Myostatin-Gene-Edited and Non-edited Cattle
title_short Comparison of Microbial Community and Metabolites in Four Stomach Compartments of Myostatin-Gene-Edited and Non-edited Cattle
title_sort comparison of microbial community and metabolites in four stomach compartments of myostatin-gene-edited and non-edited cattle
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8988179/
https://www.ncbi.nlm.nih.gov/pubmed/35401485
http://dx.doi.org/10.3389/fmicb.2022.844962
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