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TREM-1 Expression on the Surface of Neutrophils in Patients With Visceral Leishmaniasis Is Associated With Immunopathogenesis
Visceral leishmaniasis (VL) is a systemic chronic and potentially fatal disease for humans. Mechanisms related to the dysregulation of the inflammatory response may be involved in both the pathogenesis and prognosis of VL. Triggering Receptor Expressed on Myeloid Cells-1 (TREM-1) is a receptor const...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8988227/ https://www.ncbi.nlm.nih.gov/pubmed/35402286 http://dx.doi.org/10.3389/fcimb.2022.863986 |
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author | Bomfim, Lays Gisele Santos Magalhães, Lucas Sousa Rodrigues, Lorrany Santana Barreto, Aline Silva Santos, Camilla Natália Oliveira dos Santos, Priscila Lima Corrêa, Cristiane Bani Fukutani, Kiyoshi Ferreira Filho, Dalmo Correia da Silva, Angela Maria Lipscomb, Michael Wheeler de Moura, Tatiana Rodrigues |
author_facet | Bomfim, Lays Gisele Santos Magalhães, Lucas Sousa Rodrigues, Lorrany Santana Barreto, Aline Silva Santos, Camilla Natália Oliveira dos Santos, Priscila Lima Corrêa, Cristiane Bani Fukutani, Kiyoshi Ferreira Filho, Dalmo Correia da Silva, Angela Maria Lipscomb, Michael Wheeler de Moura, Tatiana Rodrigues |
author_sort | Bomfim, Lays Gisele Santos |
collection | PubMed |
description | Visceral leishmaniasis (VL) is a systemic chronic and potentially fatal disease for humans. Mechanisms related to the dysregulation of the inflammatory response may be involved in both the pathogenesis and prognosis of VL. Triggering Receptor Expressed on Myeloid Cells-1 (TREM-1) is a receptor constitutively expressed on neutrophils and monocyte subsets. The protein serves to regulate and amplify inflammatory responses. This study aimed to evaluate the expression profile of TREM-1 on the surface of neutrophils from patients with VL at varying time points during leishmanicidal treatment. For this purpose, neutrophils were isolated from the peripheral blood of patients with VL at different stages of treatment, which include 0, 7, and 30 days after treatment. Surface TREM-1 expression was assessed by immunophenotyping neutrophil populations. In addition, the association of TREM-1 expression on the surface of neutrophils with clinical and laboratory parameters and serum levels of inflammatory mediators was also evaluated. Results demonstrate a lower surface expression of TREM-1 in VL patients in the absence of treatment. However, increased levels of TREM-1 expression were observed 7 and 30 days after the start of treatment, with levels similar to those of healthy controls. TREM-1 expression was directly correlated with lymphocyte and erythrocyte count and indirectly correlated with spleen and liver size. Furthermore, elevated levels of TREM-1 expression were also correlated with lower serum levels of interleukin (IL)-22. Taken together, these results suggest that infection by Leishmania infantum leads to depressed TREM-1 expression on the neutrophil surface and may contribute to the inflammatory imbalance that characterizes active VL disease. |
format | Online Article Text |
id | pubmed-8988227 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-89882272022-04-08 TREM-1 Expression on the Surface of Neutrophils in Patients With Visceral Leishmaniasis Is Associated With Immunopathogenesis Bomfim, Lays Gisele Santos Magalhães, Lucas Sousa Rodrigues, Lorrany Santana Barreto, Aline Silva Santos, Camilla Natália Oliveira dos Santos, Priscila Lima Corrêa, Cristiane Bani Fukutani, Kiyoshi Ferreira Filho, Dalmo Correia da Silva, Angela Maria Lipscomb, Michael Wheeler de Moura, Tatiana Rodrigues Front Cell Infect Microbiol Cellular and Infection Microbiology Visceral leishmaniasis (VL) is a systemic chronic and potentially fatal disease for humans. Mechanisms related to the dysregulation of the inflammatory response may be involved in both the pathogenesis and prognosis of VL. Triggering Receptor Expressed on Myeloid Cells-1 (TREM-1) is a receptor constitutively expressed on neutrophils and monocyte subsets. The protein serves to regulate and amplify inflammatory responses. This study aimed to evaluate the expression profile of TREM-1 on the surface of neutrophils from patients with VL at varying time points during leishmanicidal treatment. For this purpose, neutrophils were isolated from the peripheral blood of patients with VL at different stages of treatment, which include 0, 7, and 30 days after treatment. Surface TREM-1 expression was assessed by immunophenotyping neutrophil populations. In addition, the association of TREM-1 expression on the surface of neutrophils with clinical and laboratory parameters and serum levels of inflammatory mediators was also evaluated. Results demonstrate a lower surface expression of TREM-1 in VL patients in the absence of treatment. However, increased levels of TREM-1 expression were observed 7 and 30 days after the start of treatment, with levels similar to those of healthy controls. TREM-1 expression was directly correlated with lymphocyte and erythrocyte count and indirectly correlated with spleen and liver size. Furthermore, elevated levels of TREM-1 expression were also correlated with lower serum levels of interleukin (IL)-22. Taken together, these results suggest that infection by Leishmania infantum leads to depressed TREM-1 expression on the neutrophil surface and may contribute to the inflammatory imbalance that characterizes active VL disease. Frontiers Media S.A. 2022-03-24 /pmc/articles/PMC8988227/ /pubmed/35402286 http://dx.doi.org/10.3389/fcimb.2022.863986 Text en Copyright © 2022 Bomfim, Magalhães, Rodrigues, Barreto, Santos, Santos, Corrêa, Fukutani, Filho, da Silva, Lipscomb and de Moura https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cellular and Infection Microbiology Bomfim, Lays Gisele Santos Magalhães, Lucas Sousa Rodrigues, Lorrany Santana Barreto, Aline Silva Santos, Camilla Natália Oliveira dos Santos, Priscila Lima Corrêa, Cristiane Bani Fukutani, Kiyoshi Ferreira Filho, Dalmo Correia da Silva, Angela Maria Lipscomb, Michael Wheeler de Moura, Tatiana Rodrigues TREM-1 Expression on the Surface of Neutrophils in Patients With Visceral Leishmaniasis Is Associated With Immunopathogenesis |
title | TREM-1 Expression on the Surface of Neutrophils in Patients With Visceral Leishmaniasis Is Associated With Immunopathogenesis |
title_full | TREM-1 Expression on the Surface of Neutrophils in Patients With Visceral Leishmaniasis Is Associated With Immunopathogenesis |
title_fullStr | TREM-1 Expression on the Surface of Neutrophils in Patients With Visceral Leishmaniasis Is Associated With Immunopathogenesis |
title_full_unstemmed | TREM-1 Expression on the Surface of Neutrophils in Patients With Visceral Leishmaniasis Is Associated With Immunopathogenesis |
title_short | TREM-1 Expression on the Surface of Neutrophils in Patients With Visceral Leishmaniasis Is Associated With Immunopathogenesis |
title_sort | trem-1 expression on the surface of neutrophils in patients with visceral leishmaniasis is associated with immunopathogenesis |
topic | Cellular and Infection Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8988227/ https://www.ncbi.nlm.nih.gov/pubmed/35402286 http://dx.doi.org/10.3389/fcimb.2022.863986 |
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