Cargando…
Systematic expression analysis of m(6)A RNA methyltransferases in clear cell renal cell carcinoma
OBJECTIVES: To investigate the regulation of the N‐6‐methyladenosine (m(6)A) methyltransferases METTL3, METTL14, WTAP, KIAA1429, and METTL4, referred to as “m(6)A writers,” in clear cell renal cell carcinoma (ccRCC), and other RCC subtypes in respect of the potential prognostic value. PATIENTS AND M...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8988738/ https://www.ncbi.nlm.nih.gov/pubmed/35474700 http://dx.doi.org/10.1002/bco2.89 |
_version_ | 1784683029944336384 |
---|---|
author | Gundert, Larissa Strick, Alexander von Hagen, Felix Schmidt, Doris Klümper, Niklas Tolkach, Yuri Toma, Marieta Kristiansen, Glen Ritter, Manuel Ellinger, Jörg |
author_facet | Gundert, Larissa Strick, Alexander von Hagen, Felix Schmidt, Doris Klümper, Niklas Tolkach, Yuri Toma, Marieta Kristiansen, Glen Ritter, Manuel Ellinger, Jörg |
author_sort | Gundert, Larissa |
collection | PubMed |
description | OBJECTIVES: To investigate the regulation of the N‐6‐methyladenosine (m(6)A) methyltransferases METTL3, METTL14, WTAP, KIAA1429, and METTL4, referred to as “m(6)A writers,” in clear cell renal cell carcinoma (ccRCC), and other RCC subtypes in respect of the potential prognostic value. PATIENTS AND METHODS: Tissue samples were collected within the framework of the Biobank at the Center for Integrated Oncology Bonn. The expression of the methyltransferases was systematically determined in clear cell renal carcinoma (ccRCC) on the RNA (real‐time PCR) and protein level (immunohistochemistry). Additionally, protein expression of the m(6)A writers was further investigated in papillary RCC, chromophobe RCC, sarcomatoid RCC, oncocytoma, and normal renal tissue (immunohistochemistry). RESULTS: The expression of all m(6)A‐methyltransferases was significantly downregulated in ccRCC compared to benign renal tissue. Low m(6)A‐methyltransferase levels were correlated with higher histological grade, advanced pT‐stage, pN‐stage, and metastatic disease. Reduced m(6)A‐methyltransferase expression was associated with shorter overall survival. CONCLUSION: In conclusion, m(6)A‐methyltransferases are dysregulated in ccRCC and might act as tumor suppressor genes, which could be of particular importance for future diagnostic and therapeutic options. |
format | Online Article Text |
id | pubmed-8988738 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-89887382022-04-25 Systematic expression analysis of m(6)A RNA methyltransferases in clear cell renal cell carcinoma Gundert, Larissa Strick, Alexander von Hagen, Felix Schmidt, Doris Klümper, Niklas Tolkach, Yuri Toma, Marieta Kristiansen, Glen Ritter, Manuel Ellinger, Jörg BJUI Compass ORIGINAL ARTICLES OBJECTIVES: To investigate the regulation of the N‐6‐methyladenosine (m(6)A) methyltransferases METTL3, METTL14, WTAP, KIAA1429, and METTL4, referred to as “m(6)A writers,” in clear cell renal cell carcinoma (ccRCC), and other RCC subtypes in respect of the potential prognostic value. PATIENTS AND METHODS: Tissue samples were collected within the framework of the Biobank at the Center for Integrated Oncology Bonn. The expression of the methyltransferases was systematically determined in clear cell renal carcinoma (ccRCC) on the RNA (real‐time PCR) and protein level (immunohistochemistry). Additionally, protein expression of the m(6)A writers was further investigated in papillary RCC, chromophobe RCC, sarcomatoid RCC, oncocytoma, and normal renal tissue (immunohistochemistry). RESULTS: The expression of all m(6)A‐methyltransferases was significantly downregulated in ccRCC compared to benign renal tissue. Low m(6)A‐methyltransferase levels were correlated with higher histological grade, advanced pT‐stage, pN‐stage, and metastatic disease. Reduced m(6)A‐methyltransferase expression was associated with shorter overall survival. CONCLUSION: In conclusion, m(6)A‐methyltransferases are dysregulated in ccRCC and might act as tumor suppressor genes, which could be of particular importance for future diagnostic and therapeutic options. John Wiley and Sons Inc. 2021-05-03 /pmc/articles/PMC8988738/ /pubmed/35474700 http://dx.doi.org/10.1002/bco2.89 Text en © 2021 The Authors. BJUI Compass published by John Wiley & Sons Ltd on behalf of BJU International Company https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | ORIGINAL ARTICLES Gundert, Larissa Strick, Alexander von Hagen, Felix Schmidt, Doris Klümper, Niklas Tolkach, Yuri Toma, Marieta Kristiansen, Glen Ritter, Manuel Ellinger, Jörg Systematic expression analysis of m(6)A RNA methyltransferases in clear cell renal cell carcinoma |
title | Systematic expression analysis of m(6)A RNA methyltransferases in clear cell renal cell carcinoma |
title_full | Systematic expression analysis of m(6)A RNA methyltransferases in clear cell renal cell carcinoma |
title_fullStr | Systematic expression analysis of m(6)A RNA methyltransferases in clear cell renal cell carcinoma |
title_full_unstemmed | Systematic expression analysis of m(6)A RNA methyltransferases in clear cell renal cell carcinoma |
title_short | Systematic expression analysis of m(6)A RNA methyltransferases in clear cell renal cell carcinoma |
title_sort | systematic expression analysis of m(6)a rna methyltransferases in clear cell renal cell carcinoma |
topic | ORIGINAL ARTICLES |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8988738/ https://www.ncbi.nlm.nih.gov/pubmed/35474700 http://dx.doi.org/10.1002/bco2.89 |
work_keys_str_mv | AT gundertlarissa systematicexpressionanalysisofm6arnamethyltransferasesinclearcellrenalcellcarcinoma AT strickalexander systematicexpressionanalysisofm6arnamethyltransferasesinclearcellrenalcellcarcinoma AT vonhagenfelix systematicexpressionanalysisofm6arnamethyltransferasesinclearcellrenalcellcarcinoma AT schmidtdoris systematicexpressionanalysisofm6arnamethyltransferasesinclearcellrenalcellcarcinoma AT klumperniklas systematicexpressionanalysisofm6arnamethyltransferasesinclearcellrenalcellcarcinoma AT tolkachyuri systematicexpressionanalysisofm6arnamethyltransferasesinclearcellrenalcellcarcinoma AT tomamarieta systematicexpressionanalysisofm6arnamethyltransferasesinclearcellrenalcellcarcinoma AT kristiansenglen systematicexpressionanalysisofm6arnamethyltransferasesinclearcellrenalcellcarcinoma AT rittermanuel systematicexpressionanalysisofm6arnamethyltransferasesinclearcellrenalcellcarcinoma AT ellingerjorg systematicexpressionanalysisofm6arnamethyltransferasesinclearcellrenalcellcarcinoma |