Cargando…

Analgesic and Anti-Arthritic Activities of Polysaccharides in Chaenomeles speciosa

Chaenomeles speciosa (Sweet) Nakai has been long used as a folk medicine for rheumatic diseases treatment. This study aimed to investigate the effects and underlying mechanism of polysaccharides in Chaenomeles speciosa (CSP) on the pro-inflammatory cytokines and MAPK pathway in complete Freund’s adj...

Descripción completa

Detalles Bibliográficos
Autores principales: Huang, Doudou, Jiang, Shenggui, Du, Zenan, Chen, Yanhong, Xue, Dan, Wang, Xiujuan, Li, Mengshuang, Zhang, Feng, Chen, Wansheng, Sun, Lianna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8989029/
https://www.ncbi.nlm.nih.gov/pubmed/35401173
http://dx.doi.org/10.3389/fphar.2022.744915
_version_ 1784683080826486784
author Huang, Doudou
Jiang, Shenggui
Du, Zenan
Chen, Yanhong
Xue, Dan
Wang, Xiujuan
Li, Mengshuang
Zhang, Feng
Chen, Wansheng
Sun, Lianna
author_facet Huang, Doudou
Jiang, Shenggui
Du, Zenan
Chen, Yanhong
Xue, Dan
Wang, Xiujuan
Li, Mengshuang
Zhang, Feng
Chen, Wansheng
Sun, Lianna
author_sort Huang, Doudou
collection PubMed
description Chaenomeles speciosa (Sweet) Nakai has been long used as a folk medicine for rheumatic diseases treatment. This study aimed to investigate the effects and underlying mechanism of polysaccharides in Chaenomeles speciosa (CSP) on the pro-inflammatory cytokines and MAPK pathway in complete Freund’s adjuvant (CFA)-induced arthritis and LPS-induced NR8383 cells. We used acetic acid (HAc)-induced writhing and CFA induced paw edema to determine the analgesic activity and anti-inflammatory activity, respectively. CFA rats were administered CSP (12.5, 25.0, and 50.0 mg/kg) daily for 3 weeks via oral gavage. The analgesic test was done using three different doses of the extract (50, 100, and 200 mg/kg). The anti-arthritic evaluation involved testing for paw swelling, swelling inhibition, and histological analysis in CFA rats. Finally, ELISA, western blot, qRT-PCR were done to determine the effect of CSP on the activation of MAPK pathway, production of pro-inflammatory cytokines in lipopolysaccharide (LPS)-stimulated NR838 macrophage cells. In pain models, oral uptake of CSP greatly reduced pain perception. Furthermore, in CFA rats, CSP substantially decreased paw swelling as well as synovial tissue proliferation and inflammatory cell infiltration. In addition, CSP was shown to inhibit pro-inflammatory cytokines (TNF-α, IL-1β, and COX-2) as well as JNK and ERK1/2 phosphorylation in LPS-stimulated NR8383 cells. Thus, pro-inflammatory cytokine secretion and MAPK signaling downregulation promoted the analgesic and anti-arthritic effects of CSP.
format Online
Article
Text
id pubmed-8989029
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-89890292022-04-08 Analgesic and Anti-Arthritic Activities of Polysaccharides in Chaenomeles speciosa Huang, Doudou Jiang, Shenggui Du, Zenan Chen, Yanhong Xue, Dan Wang, Xiujuan Li, Mengshuang Zhang, Feng Chen, Wansheng Sun, Lianna Front Pharmacol Pharmacology Chaenomeles speciosa (Sweet) Nakai has been long used as a folk medicine for rheumatic diseases treatment. This study aimed to investigate the effects and underlying mechanism of polysaccharides in Chaenomeles speciosa (CSP) on the pro-inflammatory cytokines and MAPK pathway in complete Freund’s adjuvant (CFA)-induced arthritis and LPS-induced NR8383 cells. We used acetic acid (HAc)-induced writhing and CFA induced paw edema to determine the analgesic activity and anti-inflammatory activity, respectively. CFA rats were administered CSP (12.5, 25.0, and 50.0 mg/kg) daily for 3 weeks via oral gavage. The analgesic test was done using three different doses of the extract (50, 100, and 200 mg/kg). The anti-arthritic evaluation involved testing for paw swelling, swelling inhibition, and histological analysis in CFA rats. Finally, ELISA, western blot, qRT-PCR were done to determine the effect of CSP on the activation of MAPK pathway, production of pro-inflammatory cytokines in lipopolysaccharide (LPS)-stimulated NR838 macrophage cells. In pain models, oral uptake of CSP greatly reduced pain perception. Furthermore, in CFA rats, CSP substantially decreased paw swelling as well as synovial tissue proliferation and inflammatory cell infiltration. In addition, CSP was shown to inhibit pro-inflammatory cytokines (TNF-α, IL-1β, and COX-2) as well as JNK and ERK1/2 phosphorylation in LPS-stimulated NR8383 cells. Thus, pro-inflammatory cytokine secretion and MAPK signaling downregulation promoted the analgesic and anti-arthritic effects of CSP. Frontiers Media S.A. 2022-03-24 /pmc/articles/PMC8989029/ /pubmed/35401173 http://dx.doi.org/10.3389/fphar.2022.744915 Text en Copyright © 2022 Huang, Jiang, Du, Chen, Xue, Wang, Li, Zhang, Chen and Sun. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Huang, Doudou
Jiang, Shenggui
Du, Zenan
Chen, Yanhong
Xue, Dan
Wang, Xiujuan
Li, Mengshuang
Zhang, Feng
Chen, Wansheng
Sun, Lianna
Analgesic and Anti-Arthritic Activities of Polysaccharides in Chaenomeles speciosa
title Analgesic and Anti-Arthritic Activities of Polysaccharides in Chaenomeles speciosa
title_full Analgesic and Anti-Arthritic Activities of Polysaccharides in Chaenomeles speciosa
title_fullStr Analgesic and Anti-Arthritic Activities of Polysaccharides in Chaenomeles speciosa
title_full_unstemmed Analgesic and Anti-Arthritic Activities of Polysaccharides in Chaenomeles speciosa
title_short Analgesic and Anti-Arthritic Activities of Polysaccharides in Chaenomeles speciosa
title_sort analgesic and anti-arthritic activities of polysaccharides in chaenomeles speciosa
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8989029/
https://www.ncbi.nlm.nih.gov/pubmed/35401173
http://dx.doi.org/10.3389/fphar.2022.744915
work_keys_str_mv AT huangdoudou analgesicandantiarthriticactivitiesofpolysaccharidesinchaenomelesspeciosa
AT jiangshenggui analgesicandantiarthriticactivitiesofpolysaccharidesinchaenomelesspeciosa
AT duzenan analgesicandantiarthriticactivitiesofpolysaccharidesinchaenomelesspeciosa
AT chenyanhong analgesicandantiarthriticactivitiesofpolysaccharidesinchaenomelesspeciosa
AT xuedan analgesicandantiarthriticactivitiesofpolysaccharidesinchaenomelesspeciosa
AT wangxiujuan analgesicandantiarthriticactivitiesofpolysaccharidesinchaenomelesspeciosa
AT limengshuang analgesicandantiarthriticactivitiesofpolysaccharidesinchaenomelesspeciosa
AT zhangfeng analgesicandantiarthriticactivitiesofpolysaccharidesinchaenomelesspeciosa
AT chenwansheng analgesicandantiarthriticactivitiesofpolysaccharidesinchaenomelesspeciosa
AT sunlianna analgesicandantiarthriticactivitiesofpolysaccharidesinchaenomelesspeciosa